中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

9 records (took 0.026 seconds)

    SINGLE-CELL TRANSCRIPT SEQUENCING
    1.
    发明申请
    SINGLE-CELL TRANSCRIPT SEQUENCING 审中-公开
    单细胞转录测序

    公开(公告)号：WO2018013723A1

    公开(公告)日：2018-01-18

    申请号：PCT/US2017/041770

    申请日：2017-07-12

    Applicant: FLUIDIGM CORPORATION

    Inventor： LIVAK, Kenneth J. ,  FEKETE, Richard A.

    IPC: C12Q1/68

    Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

    Abstract translation: 本文描述了用于制备用于来自细胞群的RNA的单细胞转录物测序的DNA模板的方法。 该方法需要将来自群体的细胞分布到单独的反应体积中，使得多个单独的反应体积各自含有单个分离的细胞，其中细胞在分布之前已经用固定剂处理。 然后使分离的细胞透化或破碎，并通过反转录制备cDNA，然后扩增。 还提供了用于从单细胞中的T细胞受体或免疫球蛋白有效生成DNA模板的新化学物质。

    PROBE BASED NUCLEIC ACID DETECTION
    2.
    发明申请
    PROBE BASED NUCLEIC ACID DETECTION 审中-公开
    基于探针的核酸检测

    公开(公告)号：WO2012154876A1

    公开(公告)日：2012-11-15

    申请号：PCT/US2012/037155

    申请日：2012-05-09

    Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth J. ,  MYERS, Stacey ,  WANG, Xiaohui ,  WANG, Jun

    Inventor： LIVAK, Kenneth J. ,  MYERS, Stacey ,  WANG, Xiaohui ,  WANG, Jun

    IPC: C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q2525/151

    Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

    Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

    DYNAMIC ARRAY ASSAY METHODS
    3.
    发明申请
    DYNAMIC ARRAY ASSAY METHODS 审中-公开
    动态阵列测定方法

    公开(公告)号：WO2009100449A1

    公开(公告)日：2009-08-13

    申请号：PCT/US2009/033586

    申请日：2009-02-09

    Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth J. ,  UNGER, Marc

    Inventor： LIVAK, Kenneth J. ,  UNGER, Marc

    IPC: C12M1/34

    CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q2565/629

    Abstract: High throughput methods are used that combine the features of using a matrix-type microfluidic device, labeled nucleic acid probes, and homogenous assays to detect and/or quantify nucleic acid analytes. The high throughput methods are capable of detecting nucleic acid analyes with high PCR and probe specificity, producing a low fluorescence background and therefore, a high signal to noise ratio. Additionally, the high throughput methods are capable of detecting low copy number nucleic acid analyte per cell.

    Abstract translation: 使用高通量方法，其结合使用基质型微流体装置，标记的核酸探针和均质测定的特征来检测和/或定量核酸分析物。 高通量方法能够以高PCR和探针特异性检测核酸分析，产生低荧光背景，因此具有高的信噪比。 此外，高通量方法能够检测每个细胞的低拷贝数核酸分析物。

    SINGLE-CELL TRANSCRIPT SEQUENCING
    4.
    发明申请
    SINGLE-CELL TRANSCRIPT SEQUENCING 审中-公开

    公开(公告)号：WO2018013723A9

    公开(公告)日：2018-01-18

    申请号：PCT/US2017/041770

    申请日：2017-07-12

    Applicant: FLUIDIGM CORPORATION

    Inventor： LIVAK, Kenneth J. ,  FEKETE, Richard A.

    IPC: C12Q1/68

    Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

    MULTIFUNCTIONAL PROBE-PRIMERS
    5.
    发明申请
    MULTIFUNCTIONAL PROBE-PRIMERS 审中-公开
    多功能探测器

    公开(公告)号：WO2012106668A2

    公开(公告)日：2012-08-09

    申请号：PCT/US2012/023870

    申请日：2012-02-03

    Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth J.

    Inventor： LIVAK, Kenneth J.

    IPC: E21B47/06

    CPC classification number: C12Q1/6876 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2565/1015

    Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional "self-digesting" molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5'-5' orientation.

    Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 某些方法涉及编码扩增，其中靶序列与探针结合序列和任选地与索引序列相关，（2）任选的分布步骤，其中编码扩增的产物被分成多个等分试样，和（3）a 解码和检测步骤，其中确定等分试样中靶序列的存在，不存在，数量或相对量。 检测步骤使用包含以5'-5'方向连接的引物多核苷酸和探针寡核苷酸的多功能“自消化”分子探针。

    PROBE BASED NUCLEIC ACID DETECTION
    9.
    发明公开
    PROBE BASED NUCLEIC ACID DETECTION 有权
    PROBENBASIERTERNUKLEINSÄURENACHWEIS

    公开(公告)号：EP2707507A1

    公开(公告)日：2014-03-19

    申请号：EP12782232.8

    申请日：2012-05-09

    Applicant: Fluidigm Corporation

    Inventor： LIVAK, Kenneth J. ,  MYERS, Stacey ,  WANG, Xiaohui ,  WANG, Jun

    IPC: C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q2525/151

    Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

    Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了包含调节序列和核苷酸标签识别序列的具有解链温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，扩增PCR扩增中的标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

Patent Agency Ranking