公开(公告)号：WO2013188748A1

公开(公告)日：2013-12-19

申请号：PCT/US2013/045848

申请日：2013-06-14

Applicant: FLUIDIGM CORPORATION

Inventor： LI, Nianzhen ,  UNGER, Marc

IPC: C12N5/10 ,  C12N5/02 ,  C12Q1/68 ,  C12M3/02

CPC classification number: C12N5/0618 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  C12M23/16 ,  C12N5/0619 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/12 ,  C12N2501/65 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2506/1307 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5026 ,  G01N33/57438

Abstract: This invention provides technology for transdifferentiating cells from one cell type to another. The cells are cultured with one or more vector-free gene regulator oligonucleotides concurrently or in succession, and then harvested when cell markers or the morphology of the culture shows that transdifferentiation is complete. Suitable gene regular oligonucleotides include microRNAs and messenger RNAs that encode a differentiation factor. Conditions for transdifferentiation can be optimized by dividing cells into different culture chambers of a microfluidic device. Cells are cultured with different additives in each chamber, and then compared. Transdifferentiated cells produced according to this invention can provide a consistent source of tissue for use in regenerative medicine.

Abstract translation: 本发明提供了将细胞从一种细胞类型转移到另一种细胞类型的技术。 细胞与一种或多种无载体的基因调节寡核苷酸同时或相继培养，然后当细胞标记物或培养物的形态显示转分化完成时收获细胞。 合适的基因正常寡核苷酸包括编码分化因子的微小RNA和信使RNA。 可以通过将细胞分成微流体装置的不同培养室来优化用于转分化的条件。 每个室中用不同的添加剂培养细胞，然后进行比较。 根据本发明生产的转分化细胞可提供用于再生医学的一致的组织来源。