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    TECHNOLOGY FOR CELL TRANSDIFFERENTIATION
    2.
    发明申请
    TECHNOLOGY FOR CELL TRANSDIFFERENTIATION 审中-公开
    细胞转导技术

    公开(公告)号：WO2013188748A1

    公开(公告)日：2013-12-19

    申请号：PCT/US2013/045848

    申请日：2013-06-14

    Applicant: FLUIDIGM CORPORATION

    Inventor： LI, Nianzhen ,  UNGER, Marc

    IPC: C12N5/10 ,  C12N5/02 ,  C12Q1/68 ,  C12M3/02

    CPC classification number: C12N5/0618 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  C12M23/16 ,  C12N5/0619 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/12 ,  C12N2501/65 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2506/1307 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5026 ,  G01N33/57438

    Abstract: This invention provides technology for transdifferentiating cells from one cell type to another. The cells are cultured with one or more vector-free gene regulator oligonucleotides concurrently or in succession, and then harvested when cell markers or the morphology of the culture shows that transdifferentiation is complete. Suitable gene regular oligonucleotides include microRNAs and messenger RNAs that encode a differentiation factor. Conditions for transdifferentiation can be optimized by dividing cells into different culture chambers of a microfluidic device. Cells are cultured with different additives in each chamber, and then compared. Transdifferentiated cells produced according to this invention can provide a consistent source of tissue for use in regenerative medicine.

    Abstract translation: 本发明提供了将细胞从一种细胞类型转移到另一种细胞类型的技术。 细胞与一种或多种无载体的基因调节寡核苷酸同时或相继培养，然后当细胞标记物或培养物的形态显示转分化完成时收获细胞。 合适的基因正常寡核苷酸包括编码分化因子的微小RNA和信使RNA。 可以通过将细胞分成微流体装置的不同培养室来优化用于转分化的条件。 每个室中用不同的添加剂培养细胞，然后进行比较。 根据本发明生产的转分化细胞可提供用于再生医学的一致的组织来源。

    DYNAMIC ARRAY ASSAY METHODS
    4.
    发明申请
    DYNAMIC ARRAY ASSAY METHODS 审中-公开
    动态阵列测定方法

    公开(公告)号：WO2009100449A1

    公开(公告)日：2009-08-13

    申请号：PCT/US2009/033586

    申请日：2009-02-09

    Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth J. ,  UNGER, Marc

    Inventor： LIVAK, Kenneth J. ,  UNGER, Marc

    IPC: C12M1/34

    CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q2565/629

    Abstract: High throughput methods are used that combine the features of using a matrix-type microfluidic device, labeled nucleic acid probes, and homogenous assays to detect and/or quantify nucleic acid analytes. The high throughput methods are capable of detecting nucleic acid analyes with high PCR and probe specificity, producing a low fluorescence background and therefore, a high signal to noise ratio. Additionally, the high throughput methods are capable of detecting low copy number nucleic acid analyte per cell.

    Abstract translation: 使用高通量方法，其结合使用基质型微流体装置，标记的核酸探针和均质测定的特征来检测和/或定量核酸分析物。 高通量方法能够以高PCR和探针特异性检测核酸分析，产生低荧光背景，因此具有高的信噪比。 此外，高通量方法能够检测每个细胞的低拷贝数核酸分析物。

    HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS
    5.
    发明申请
    HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS 审中-公开
    通过DIGITIZED TRANSPOSONS进行快速测定

    公开(公告)号：WO2015179706A1

    公开(公告)日：2015-11-26

    申请号：PCT/US2015/032066

    申请日：2015-05-21

    Applicant: FLUIDIGM CORPORATION

    Inventor： XI, Lei ,  WANG, Xiaohui ,  UNGER, Marc ,  RUFF, David

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1082 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q2525/155 ,  C12Q2525/301 ,  C12Q2535/122

    Abstract: In certain embodiments, the present invention provides a way of "digitally" marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

    Abstract translation: 在某些实施方案中，本发明提供了一种通过使用转座酶在进一步分析之前将不同的条形码转座子插入基因组DNA来“不同”地标记不同染色体等位基因的方法。 根据这种方法，每个等位基因都被标记为转座子条形码的独特模式。 因为转座子条形码的每个独特模式识别特定的等位基因，所以该方法有助于确定倍性和拷贝数变异，提高了鉴别纯合子，杂合子和测序错误引起的模式的能力，并且允许通过DNA的非信息延伸分离的位点 被确定为相关基因座，从而促进单倍型测定。 还提供了一种新颖的人工转座子末端，其包括对于转座不是必需的两个或更多个位置的条形码序列。

    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME
    7.
    发明申请
    CRYSTAL FORMING DEVICES AND SYSTEMS AND METHODS FOR MAKING AND USING THE SAME 审中-公开
    晶体形成装置和系统及其制造和使用方法

    公开(公告)号：WO2005072353A2

    公开(公告)日：2005-08-11

    申请号：PCT/US2005/002408

    申请日：2005-01-25

    Applicant: FLUIDIGM CORPORATION ,  GROSSMAN, Robert ,  UNGER, Marc ,  LAM, Phillip ,  CHOU, Hou-Pu ,  KIMBALL, Jake ,  PIEPRZYK, Martin

    Inventor： GROSSMAN, Robert ,  UNGER, Marc ,  LAM, Phillip ,  CHOU, Hou-Pu ,  KIMBALL, Jake ,  PIEPRZYK, Martin

    IPC: B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00 ,  C30B7/00 ,  C30B29/58

    CPC classification number: C12Q1/68 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2400/0487 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T436/11

    Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

    Abstract translation: 本发明提供了微流体装置及其使用方法。 本发明还提供了用于使用微流体装置的装置和系统，分析在微流体装置中进行的反应，以及用于生成，存储，组织和分析使用微流体装置产生的数据的系统。 本发明还提供了使用和制造微流体系统和装置的方法，在一些实施方案中，它们可用于晶体形成。 在一个实施例中，一种装置包括具有其中具有一个或多个流体端口的压板面的压板。 流体端口在空间上对应于微流体装置表面上的一个或多个孔。 包括用于相对于压板保持微流体装置的平台，以及用于将压板压靠在微流体装置上的压板致动器，使得压板的至少一个流体端口被推压到一个井中以形成压力室 包括井和端口，使得当压力流体通过其中一个端口被引入或从压力室移除时，流体压力在其中改变。

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