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    NUCLEIC ACID ENCODING REACTIONS
    5.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 审中-公开
    核酸编码反应

    公开(公告)号：WO2012162267A2

    公开(公告)日：2012-11-29

    申请号：PCT/US2012/038894

    申请日：2012-05-21

    Applicant: FLUIDIGM CORPORATION ,  ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  JONES, Robert C. ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  JONES, Robert C. ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C40B20/00 ,  C40B50/06 ,  C40B40/06 ,  C40B30/04 ,  C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。 在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。 在其它实施方案中，关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中，本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS
    6.
    发明申请
    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 审中-公开
    用于靶向靶核酸的多重PCR扩增方法

    公开(公告)号：WO2010115154A1

    公开(公告)日：2010-10-07

    申请号：PCT/US2010/029854

    申请日：2010-04-02

    Applicant: FLUIDIGM CORPORATION ,  MAY, Andrew ,  CHEN, Peilin ,  WANG, Jun ,  KAPER, Fiona ,  ANDERSON, Megan

    Inventor： MAY, Andrew ,  CHEN, Peilin ,  WANG, Jun ,  KAPER, Fiona ,  ANDERSON, Megan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    Abstract translation: 在某些实施方案中，本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。 在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    7.
    发明申请
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 审中-公开
    颗粒群体的单粒子分析

    公开(公告)号：WO2013177206A2

    公开(公告)日：2013-11-28

    申请号：PCT/US2013/042086

    申请日：2013-05-21

    Applicant: FLUIDIGM CORPORATION ,  ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out "in parallel," i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 当颗粒处于分开的反应体积中时，可以测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，“并行地”执行一个或多个参数测量，即基本上同时在单独的反应体积中进行。

    MICROFLUIDIC DEVICES WITH REMOVABLE COVER AND METHODS OF FABRICATION AND APPLICATION
    9.
    发明申请
    MICROFLUIDIC DEVICES WITH REMOVABLE COVER AND METHODS OF FABRICATION AND APPLICATION 审中-公开
    具有可拆卸盖的微流体装置以及制造和应用方法

    公开(公告)号：WO2011040884A2

    公开(公告)日：2011-04-07

    申请号：PCT/SG2010/000369

    申请日：2010-09-29

    Applicant: FLUIDIGM CORPORATION ,  COHEN, David ,  MAY, Andrew ,  PIEPRZYK, Martin ,  FOWLER, Brian ,  LEE, Kim Huat ,  YAN, Jun ,  ZHOU, Ming Fang ,  NG, Seng Beng

    Inventor： COHEN, David ,  MAY, Andrew ,  PIEPRZYK, Martin ,  FOWLER, Brian ,  LEE, Kim Huat ,  YAN, Jun ,  ZHOU, Ming Fang ,  NG, Seng Beng

    IPC: G01N35/08 ,  G01N33/48

    CPC classification number: B01L3/5027 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/12 ,  B01L2300/045 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0655 ,  G01N1/405

    Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.

    Abstract translation: 本发明包括具有微型空腔的​​微流体系统，其可以被可移除的盖覆盖，其中可移除的盖允许微加工腔的至少一部分开口被操作者暴露或直接访问。 微流体系统包括在可包括PDMS的弹性体层中形成的腔室，流动和控制通道。 可移除的盖包括通过粘合剂层结合到弹性体层的热塑性基底膜。 当可移除的盖被剥离时，室至少部分地打开以允许从室中取样。 该室可能在PCR反应内部形成大分子晶体或产生内含物。 本发明还包括通过使用可移除盖制造弹性体层中的通孔的方法。 本发明还包括用于剥离可剥离盖或诸如集成式加热器撒布机的可移除部件的方法和装置。

    METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING
    10.
    发明申请
    METHOD AND SYSTEM FOR CRYSTALLIZATION AND X-RAY DIFFRACTION SCREENING 审中-公开

    公开(公告)号：WO2008141183A3

    公开(公告)日：2008-11-20

    申请号：PCT/US2008/063247

    申请日：2008-05-09

    Applicant: FLUIDIGM CORPORATION ,  FOWLER, Brian ,  MAY, Andrew

    Inventor： FOWLER, Brian ,  MAY, Andrew

    IPC: H05G1/00

    Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

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