中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

15 records (took 0.026 seconds)

    MICROFLUIDIC CHECK VALVES
    6.
    发明申请
    MICROFLUIDIC CHECK VALVES 审中-公开
    微流感检查阀

    公开(公告)号：WO2008043046A2

    公开(公告)日：2008-04-10

    申请号：PCT/US2007/080489

    申请日：2007-10-04

    Applicant: FLUIDIGM CORPORATION ,  WANG, Jing ,  NASSEF, Hany, R.

    Inventor： WANG, Jing ,  NASSEF, Hany, R.

    IPC: B01J19/00

    CPC classification number: F16K99/0026 ,  B01L3/502738 ,  B01L2200/12 ,  B01L2300/0887 ,  B01L2400/0605 ,  B01L2400/0638 ,  F16K99/0001 ,  F16K99/0057 ,  F16K2099/0078 ,  F16K2099/008

    Abstract: The present invention is a robust, microfluidic check valve and a method of using the check valve in microfluidic devices. The check valve is comprised two stacked chambers that are separated by a pore-containing membrane. The membrane is composed of an elastomeric material and can be configured in normally open or normally closed state. The normally open check valve can be implemented so that the degree of back pressure necessary to close the valve can be set. The normally closed embodiment can maintain a closed state with essentially no back pressure. Both the normally open and the normally closed version can be readily produced by multilayer soft lithographic techniques and may retain effective functioning through many thousands of opening and closing cycles without failure. Such check valves can substitute for active valve structures in microfluidic devices and, when appropriately implemented, can simplify the design, manufacture, and/or operation of the devices containing them.

    Abstract translation: 本发明是一种坚固的微流体止回阀和在微流体装置中使用止回阀的方法。 止回阀包括由含孔膜隔开的两个堆叠室。 膜由弹性体材料构成，并且可以构造成常开或常闭状态。 可以实现常开止回阀，使得可以设定关闭阀所需的背压程度。 常闭实施例可以保持基本上没有背压的关闭状态。 常开和常闭型都可以通过多层软平版印刷技术轻松生产，并可通过数千个开闭周期保持有效的功能，而不会发生故障。 这种止回阀可以替代微流体装置中的主动阀结构，并且当适当实施时，可以简化包含它们的装置的设计，制造和/或操作。

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    7.
    发明公开
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 审中-公开
    使用微流控芯片进行多个单细胞捕获和处理的方法，系统和设备

    公开(公告)号：EP3285062A1

    公开(公告)日：2018-02-21

    申请号：EP17180284.6

    申请日：2013-02-28

    Applicant: Fluidigm Corporation

    Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

    IPC: G01N15/10

    CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    Abstract translation: 描述了使用微流体的多个单细胞捕获和处理的方法，系统和设备。 提供了用于捕获，分配和/或操纵来自更大细胞群体的个体细胞以及产生与每个个体细胞相关的遗传信息和/或反应的工具和技术。 可以使用不同的捕获配置来捕获单个细胞，然后在多腔室反应配置中处理每个单独的细胞。 一些实施例可以提供特定目标扩增，全基因组扩增，全转录扩增，实时PCR制备，拷贝数变异，预扩增的mRNA的测序，和/或单倍型分析已经从较大人口划分的多个单个细胞的 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置用于将单独的单元或相关联的反应产物成像为处理的一部分。 反应产物可能在一些情况下收获和/或进一步分析。

Patent Agency Ranking