公开(公告)号：WO2021168439A1

公开(公告)日：2021-08-26

申请号：PCT/US2021/019101

申请日：2021-02-22

Applicant: FLUIDIGM CORPORATION

Inventor： PHELAN, Michael L. ,  KUBU, Christopher J. ,  FOWLER, Brian ,  SUN, Gang ,  PATEL, Nikita ,  RAMALINGAM, Naveen

IPC: C12P19/34 ,  B01F11/00 ,  B01F11/02

Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.