公开(公告)号：WO2017005663A1

公开(公告)日：2017-01-12

申请号：PCT/EP2016/065612

申请日：2016-07-01

Applicant: FOUNDATION FOR RESEARCH AND TECHNOLOGY HELLAS

Inventor： PAPADAKIS, George ,  GIZELI, Electra

IPC: G01N29/02 ,  G01N29/032 ,  C12Q1/68 ,  G01N29/036 ,  G01N33/543

CPC classification number: G01N29/022 ,  C12Q1/6825 ,  C12Q2565/634 ,  G01N29/032 ,  G01N29/036 ,  G01N33/54373 ,  G01N33/586 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0257 ,  G01N2291/0426 ,  C12Q2525/197

Abstract: A sensitive assay for an analyte employing an acoustic wave sensor. A label which has a higher dissipative capacity than the analyte is adhered to the sensing surface of an acoustic wave sensor through the analyte such that the body of the label is spaced apart from and anchored to the surface of the acoustic wave sensor by a distance of 15 to 250nm. The change in the energy losses or the frequency or phase of the acoustic wave when the label binds to the sensing surface is used to measure the presence or amount of the label. A substantial improvement in the detection limit of the label is obtained. The analyte may for example be a nucleic acid and the label may for example comprise liposomes.

Abstract translation: 使用声波传感器的分析物的敏感测定。 具有比分析物更高的耗散能力的标签通过分析物粘附到声波传感器的感测表面，使得标签的主体与声波传感器的表面间隔开并锚定在声波传感器的表面上 15至250nm。 当标签与感测表面结合时，能量损失或声波频率或相位的变化用于测量标签的存在或数量。 获得标签检测限的实质性改进。 分析物可以例如是核酸，并且标记可以例如包括脂质体。

公开(公告)号：EP3317656A1

公开(公告)日：2018-05-09

申请号：EP16734648.5

申请日：2016-07-01

Applicant: Foundation For Research And Technology Hellas

Inventor： PAPADAKIS, George ,  GIZELI, Electra

IPC: G01N29/02 ,  G01N29/032 ,  C12Q1/68 ,  G01N29/036 ,  G01N33/543

CPC classification number: G01N29/022 ,  C12Q1/6825 ,  C12Q2565/634 ,  G01N29/032 ,  G01N29/036 ,  G01N33/54373 ,  G01N33/586 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0257 ,  G01N2291/0426 ,  C12Q2525/197

Abstract: A sensitive assay for an analyte employing an acoustic wave sensor. A label which has a higher dissipative capacity than the analyte is adhered to the sensing surface of an acoustic wave sensor through the analyte such that the body of the label is spaced apart from and anchored to the surface of the acoustic wave sensor by a distance of 15 to 250 nm. The change in the energy losses of the acoustic wave when the label binds to the sensing surface is used to measure the presence or amount of the label. A substantial improvement in the detection limit of the label is obtained. The analyte may for example be a nucleic acid and the label may for example comprise liposomes.