公开(公告)号：WO2020089399A1

公开(公告)日：2020-05-07

申请号：PCT/EP2019/079845

申请日：2019-10-31

Applicant: FOUNDATION FOR RESEARCH AND TECHNOLOGY HELLAS

Inventor： PAPADAKIS, Georgios ,  GIZELI, Electra ,  PANTAZIS, Alexandros

IPC: C12Q1/6851 ,  G16B99/00 ,  G16B40/10

Abstract: Method and apparatus for performing a real-time colorimetric nucleic acid amplification assay wherein the heating of the liquid sample comprised in a reaction tube is carried out by bringing the bottom of the tube in thermal contact with a heating element. The real-time monitoring of the content of the reaction tube is carried out visually through the side wall of the tube, preferably by using a camera.

公开(公告)号：WO2008155692A3

公开(公告)日：2008-12-24

申请号：PCT/IB2008/052323

申请日：2008-06-12

Applicant: FOUNDATION FOR RESEARCH AND TECHNOLOGY HELLAS ,  TSORTOS, Achilleas ,  PAPADAKIS, Georgios ,  GIZELI, Electra

Inventor： TSORTOS, Achilleas ,  PAPADAKIS, Georgios ,  GIZELI, Electra

IPC: C12Q1/68 ,  G01N29/00 ,  G01N33/00

Abstract: Disclosed is a method of investigating the conformation of biomolecules or changes in the conformation of biomolecules as a result of an interaction, in which biomolecules from a sample of biomolecules are adhered discretely to the sensing surface of an acoustic wave sensor operating in a liquid, and a conformation parameter which is related to the conformation of the biomolecules from the said sample which are adhered discretely to the sensing surface, but substantially independent of the resulting change in mass loading of the sensing surface, is calculated from the resulting change in the output signals of the acoustic wave sensor. The conformation parameter may the acoustic ratio or a parameter which is directly related to the acoustic ratio. There is also disclosed a method of investigating changes in the conformation of biomolecules which are adhered discretely to the sensing surface of an acoustic wave sensor operating in a liquid, in response to an interaction with an agent, by calculating a conformation parameter which is related to the change in conformation of biomolecules adhered discretely to the sensing surface as a result of an interaction with the agent, but which is substantially independent of any resulting change in mass loading of the sensing surface.

公开(公告)号：EP2171083B1

公开(公告)日：2014-03-12

申请号：EP08763311.1

申请日：2008-06-12

Applicant: Foundation For Research And Technology Hellas

Inventor： TSORTOS, Achilleas ,  PAPADAKIS, Georgios ,  GIZELI, Electra

IPC: C12Q1/68 ,  G01N29/00 ,  G01N33/00

CPC classification number: G01N33/54373 ,  C12Q1/6811 ,  G01N29/022 ,  G01N29/4427 ,  G01N2291/014 ,  G01N2291/015 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/02818 ,  G01N2500/00 ,  C12Q2565/634

公开(公告)号：EP3874063A1

公开(公告)日：2021-09-08

申请号：EP19794164.4

申请日：2019-10-31

Applicant: Foundation For Research And Technology Hellas

Inventor： PAPADAKIS, Georgios ,  GIZELI, Electra ,  PANTAZIS, Alexandros

IPC: C12Q1/6851 ,  G16B99/00 ,  G16B40/10

公开(公告)号：EP2171083A2

公开(公告)日：2010-04-07

申请号：EP08763311.1

申请日：2008-06-12

Applicant: Foundation For Research And Technology Hellas

Inventor： TSORTOS, Achilleas ,  PAPADAKIS, Georgios ,  GIZELI, Electra

IPC: C12Q1/68 ,  G01N29/00 ,  G01N33/00

CPC classification number: G01N33/54373 ,  C12Q1/6811 ,  G01N29/022 ,  G01N29/4427 ,  G01N2291/014 ,  G01N2291/015 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/02818 ,  G01N2500/00 ,  C12Q2565/634

Abstract: Disclosed is a method of investigating the conformation of biomolecules or changes in the conformation of biomolecules as a result of an interaction, in which biomolecules from a sample of biomolecules are adhered discretely to the sensing surface of an acoustic wave sensor operating in a liquid, and a conformation parameter which is related to the conformation of the biomolecules from the said sample which are adhered discretely to the sensing surface, but substantially independent of the resulting change in mass loading of the sensing surface, is calculated from the resulting change in the output signals of the acoustic wave sensor. The conformation parameter may the acoustic ratio or a parameter which is directly related to the acoustic ratio. There is also disclosed a method of investigating changes in the conformation of biomolecules which are adhered discretely to the sensing surface of an acoustic wave sensor operating in a liquid, in response to an interaction with an agent, by calculating a conformation parameter which is related to the change in conformation of biomolecules adhered discretely to the sensing surface as a result of an interaction with the agent, but which is substantially independent of any resulting change in mass loading of the sensing surface.