公开(公告)号：KR840002294B1

公开(公告)日：1984-12-15

申请号：KR810002646

申请日：1981-07-21

Applicant: FUJISAWA PHARMACEUTICAL CO

Inventor： IMANAKA HIROSHI ,  MIYOSHI TOSHIO ,  KONOMI TOSHIO ,  KUBOCHI YOSHIAKI ,  HATTORI SEIZIRO ,  KAWAKITA TAKESHI

IPC: C12N9/18 ,  C12P35/06 ,  C12P39/00 ,  C12R1/645 ,  C12R1/745

公开(公告)号：JPH0249580A

公开(公告)日：1990-02-19

申请号：JP8085989

申请日：1989-03-30

Applicant: FUJISAWA PHARMACEUTICAL CO

Inventor： IMANAKA HIROSHI ,  MIYOSHI TOSHIO ,  MOTOI SATOO ,  KUBOCHI YOSHIAKI ,  HATTORI SEIJIRO ,  KAWAKITA TAKESHI

IPC: C12N9/18 ,  C12P35/06 ,  C12P39/00 ,  C12R1/645 ,  C12R1/745

Abstract: NEW MATERIAL:Acetyl esterase 4466-I and 4466-II having the following properties. Hydrolysis activity of cephalosporin C to deacetylcephalosporin C, positive; substrate specificity, hydrolyzable substrate is acetic acid ester; optimum pH, , Mn , Zn , Ni , Ca , Ba and Fe ; Km value, 11.5mM for 4466-I and 13.7mM for 4466-II; molecular weight (gel-permeation), 780,000 Dalton for 4466-I and 920,000 Dalton for 4466-II. USE:The enzyme deacetylates cephalosporin C to give deacetylcephalosporin C useful as a medicine in remarkably high yield. PREPARATION:The objective enzyme can be produced, e.g., by culturing Aureobasidium pullulans IFO 4466 at 25 deg.C and pH5-8 for 24-50hr under aeration and agitation.

公开(公告)号：JPH0147158B2

公开(公告)日：1989-10-12

申请号：JP11430681

申请日：1981-07-20

Applicant: FUJISAWA PHARMACEUTICAL CO

Inventor： IMANAKA HIROSHI ,  MYOSHI TOSHIO ,  MOTOI SATOO ,  KUBOCHI YOSHIAKI ,  HATSUTORI SEIJIRO ,  KAWAKITA TAKESHI

IPC: C12N9/18 ,  C12P35/06 ,  C12P39/00 ,  C12R1/645 ,  C12R1/745

公开(公告)号：JPH0251594B2

公开(公告)日：1990-11-07

申请号：JP8085989

申请日：1989-03-30

Applicant: FUJISAWA PHARMACEUTICAL CO

Inventor： IMANAKA HIROSHI ,  MYOSHI TOSHIO ,  MOTOI SATOO ,  KUBOCHI YOSHIAKI ,  HATSUTORI SEIJIRO ,  KAWAKITA TAKESHI

IPC: C12N9/18 ,  C12P35/06 ,  C12P39/00 ,  C12R1/645 ,  C12R1/745

公开(公告)号：DE3170118D1

公开(公告)日：1985-05-30

申请号：DE3170118

申请日：1981-07-20

Applicant: FUJISAWA PHARMACEUTICAL CO

Inventor： IMANAKA HIROSHI ,  MIYOSHI TOSHIO ,  KONOMI TOSHIO ,  KUBOCHI YOSHIAKI ,  HATTORI SEIZIRO ,  KAWAKITA TAKESHI

IPC: C12N9/18 ,  C12P35/06 ,  C12P39/00 ,  C12R1/645 ,  C12R1/745

Abstract: Deacetylcephalosporin C is prepared by contacting cephalosporin C with an acetylesterase produced by a strain belonging to the genus Auresbasideum or a mutant thereof. Deacetylcephalosporin C is known as a valuable intermediate in syntheses of therapeutically-useful cephalosporin compounds.

公开(公告)号：AT12950T

公开(公告)日：1985-05-15

申请号：AT81303313

申请日：1981-07-20

Applicant: FUJISAWA PHARMACEUTICAL CO

Inventor： IMANAKA HIROSHI ,  MIYOSHI TOSHIO ,  KONOMI TOSHIO ,  KUBOCHI YOSHIAKI ,  HATTORI SEIZIRO ,  KAWAKITA TAKESHI

IPC: C12N9/18 ,  C12P35/06 ,  C12P39/00 ,  C12R1/645 ,  C12R1/745

Abstract: Deacetylcephalosporin C is prepared by contacting cephalosporin C with an acetylesterase produced by a strain belonging to the genus Auresbasideum or a mutant thereof. Deacetylcephalosporin C is known as a valuable intermediate in syntheses of therapeutically-useful cephalosporin compounds.