公开(公告)号：EP1233026A4

公开(公告)日：2005-08-17

申请号：EP00969985

申请日：2000-10-23

Applicant: FUSO PHARMACEUTICAL IND

Inventor： KUWAHARA YOSHIHIRO ,  HASEGAWA MICHINORI ,  ISAKA KIYOTSUGU ,  ARAKI HIROMASA

IPC: C07K16/18 ,  C12N5/20 ,  C12N15/06 ,  C12P21/08 ,  G01N33/577 ,  G01N33/68 ,  C12N5/10 ,  G01N33/15 ,  G01N33/53

CPC classification number: C07K16/18 ,  G01N33/689 ,  G01N2800/367 ,  Y10S435/806 ,  Y10S435/975

Abstract: An antibody binding specifically to rat postacrosome reaction sperm is produced and hybridomas (FARS-91 and FARS-92 strains) capable of stably proliferating are obtained by fusing mouse spleen cells having a high antibody titer against rat postacrosome reaction sperm with mouse-origin myeloma cells and screening fused cells reacting strongly with rat postacrosome reaction sperm. From these hybridomas, monoclonal antibodies selectively binding to rat postacrosome reaction sperm can be obtained. Thus, a diagnostic method for evaluating rat fertility is presented.

公开(公告)号：AU7954000A

公开(公告)日：2001-05-08

申请号：AU7954000

申请日：2000-10-23

Applicant: FUSO PHARMACEUTICAL IND

Inventor： KUWAHARA YOSHIHIRO ,  HASEGAWA MICHINORI ,  ISAKA KIYOTSUGU ,  ARAKI HIROMASA

IPC: C07K16/18 ,  C12N5/20 ,  C12N15/06 ,  C12P21/08 ,  G01N33/577 ,  G01N33/68 ,  C12N5/10 ,  G01N33/15 ,  G01N33/53

Abstract: An antibody biding specifically to rat's acrosome reacted sperm is produced and hybridomas (FARS-91 and FARS-92 strains ) capable of stably proliferating are obtained by fusing mouse spleen cells having a high antibody titer against rat's acrosome reacted sperm with mouse-origin myeloma cells and screening fused cells reacting strongly with rat's acrosome reacted sperm. From these hybridomas, monoclonal antibodies selectively binding to rat's acrosome reacted sperm can be obtained. Thus, a diagnostic method for evaluating fertility of rat's spermatozoa is presented.