公开(公告)号：AU2004312893B2

公开(公告)日：2010-06-17

申请号：AU2004312893

申请日：2004-12-29

Applicant: HARVARD COLLEGE

Inventor： LINDER VINCENT ,  PARVIZ BABAK A ,  WHITESIDES GEORGE M ,  SIEGEL ADAM ,  SIA SAMUEL K

IPC: B01L3/00 ,  G01N21/05 ,  G01N21/53 ,  G01N21/59 ,  G01N33/53 ,  G01N33/58

Abstract: An assay device and method are described. The assay may involve immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.

公开(公告)号：AU2004312893A1

公开(公告)日：2005-07-21

申请号：AU2004312893

申请日：2004-12-29

Applicant: HARVARD COLLEGE

Inventor： LINDER VINCENT ,  PARVIZ BABAK A ,  WHITESIDES GEORGE M ,  SIEGEL ADAM ,  SIA SAMUEL K

IPC: B01L3/00 ,  G01N21/05 ,  G01N21/53 ,  G01N21/59 ,  G01N33/53 ,  G01N33/58

公开(公告)号：CA2834041C

公开(公告)日：2017-05-16

申请号：CA2834041

申请日：2004-12-29

Applicant: HARVARD COLLEGE

Inventor： SIA SAMUEL K ,  LINDER VINCENT ,  PARVIZ BABAK A ,  SIEGEL ADAM ,  WHITESIDES GEORGE M

IPC: G01N33/543 ,  B01L3/00 ,  B81B1/00 ,  G01N21/05 ,  G01N21/53 ,  G01N21/59 ,  G01N33/53 ,  G01N33/58

Abstract: An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold- conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.

公开(公告)号：CA2834041A1

公开(公告)日：2005-07-21

申请号：CA2834041

申请日：2004-12-29

Applicant: HARVARD COLLEGE

Inventor： SIA SAMUEL K ,  LINDER VINCENT ,  PARVIZ BABAK A ,  SIEGEL ADAM ,  WHITESIDES GEORGE M

IPC: G01N33/543 ,  B01L3/00 ,  B81B1/00 ,  G01N21/05 ,  G01N21/53 ,  G01N21/59 ,  G01N33/53 ,  G01N33/58

公开(公告)号：CA2564609C

公开(公告)日：2014-02-11

申请号：CA2564609

申请日：2004-12-29

Applicant: HARVARD COLLEGE

Inventor： SIA SAMUEL K ,  LINDER VINCENT ,  PARVIZ BABAK A ,  SIEGEL ADAM ,  WHITESIDES GEORGE M

IPC: G01N21/53 ,  B01L3/00 ,  G01N21/05 ,  G01N21/59 ,  G01N33/53 ,  G01N33/58

Abstract: An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion (140) of a surface (130) of a microfluidic chamber (120,122,124); passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.