公开(公告)号：WO2007140961A8

公开(公告)日：2008-11-06

申请号：PCT/EP2007004923

申请日：2007-06-04

Applicant: ROCHE DIAGNOSTICS GMBH ,  HOFFMANN LA ROCHE ,  KOBOLD UWE ,  DUELFFER THOMAS ,  HERRMANN RUPERT ,  ELTZ HERBERT VON DER

Inventor： KOBOLD UWE ,  DUELFFER THOMAS ,  HERRMANN RUPERT ,  VON DER ELTZ HERBERT

IPC: G01N30/06 ,  G01N1/38 ,  G01N30/02 ,  G01N30/60 ,  G01N30/72 ,  G01N33/50

CPC classification number: G01N30/06 ,  G01N30/603 ,  G01N30/7233 ,  G01N33/5002 ,  G01N2030/027 ,  Y10T436/24 ,  Y10T436/25

Abstract: The invention relates to a method for differentially hemolyzing whole blood. It discloses method for detecting an analyte in a liquid sample known or suspected to comprise red blood cells and suspected or known to comprise eukaryotic cells, the method comprising the steps of processing said liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may comprise both erythrocytes as well as nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.

Abstract translation: 本发明涉及一种使全血不同溶血的方法。 它公开了用于检测已知或怀疑包含红细胞并且怀疑或已知包含真核细胞的液体样品中的分析物的方法，所述方法包括以下步骤：在适合于裂解细胞膜的条件下用膜增溶剂处理所述液体样品 的红细胞，同时不会引起样品成分沉淀，对经处理的样品进行色谱分离，并检测分析物。 在检测可能包含红细胞和有核细胞的液体样品中的分析物的方法中，红细胞的差异溶血是有利的。 红细胞的差异溶解可以容易地与诸如LC-MS的在线检测方法组合，并且在许多分析物的检测中是有利的，例如， 在检测叶酸或免疫抑制药物，如他克莫司或西罗莫司。

公开(公告)号：WO2007140962A3

公开(公告)日：2008-01-31

申请号：PCT/EP2007004924

申请日：2007-06-04

Applicant: ROCHE DIAGNOSTICS GMBH ,  HOFFMANN LA ROCHE ,  KOBOLD UWE ,  DUELFFER THOMAS ,  GROL MICHAEL ,  HERRMANN RUPERT ,  ELTZ HERBERT VON DER ,  VON PROFF LEOPOLD

Inventor： KOBOLD UWE ,  DUELFFER THOMAS ,  GROL MICHAEL ,  HERRMANN RUPERT ,  VON DER ELTZ HERBERT ,  VON PROFF LEOPOLD

IPC: G01N33/82

CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/203332

Abstract: The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

Abstract translation: 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。

公开(公告)号：CA2649666C

公开(公告)日：2011-07-05

申请号：CA2649666

申请日：2007-06-04

Applicant: HOFFMANN LA ROCHE

Inventor： NASER WERNER ,  DUELFFER THOMAS ,  HERRMANN RUPERT ,  KOBOLD UWE ,  VON DER ELTZ HERBERT

IPC: G01N33/50

Abstract: The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.

公开(公告)号：ES2336382T3

公开(公告)日：2010-04-12

申请号：ES07725793

申请日：2007-06-04

Applicant: HOFFMANN LA ROCHE

Inventor： KOBOLD UWE ,  DUELFFER THOMAS ,  GROL MICHAEL ,  HERRMANN RUPERT ,  VON DER ELTZ HERBERT ,  VON PROFF LEOPOLD

IPC: G01N33/82

Abstract: Método para la medición de un metabolito de la vitamina D en una muestra, comprendiendo el método las etapas siguientes: a) tratar dicha muestra con un reactivo liberador bajo condiciones apropiadas para liberar el metabolito de la vitamina D respecto de la proteína ligante de vitamina D y no provocar la precipitación de proteínas, b) someter la muestra tratada que se ha obtenido en la etapa (a) a cromatografía líquida, y c) medir el metabolito de la vitamina D durante o después de la cromatografía líquida, en el que dicho reactivo liberador se basa en una sal que presenta un N-heterociclo cuaternario a modo de catión.

公开(公告)号：CA2649633C

公开(公告)日：2012-11-27

申请号：CA2649633

申请日：2007-06-04

Applicant: HOFFMANN LA ROCHE

Inventor： KOBOLD UWE ,  DUELFFER THOMAS ,  HERRMANN RUPERT ,  VON DER ELTZ HERBERT

IPC: G01N30/06 ,  G01N1/38 ,  G01N30/02 ,  G01N30/60 ,  G01N30/72 ,  G01N33/50

Abstract: The invention relates to a method for differentially hemolyzing whole blood. It discloses method for detecting an analyte in a liquid sample known or suspected to comprise red blood cells and suspected or known to comprise eukaryotic cells, the method comprising the steps of processing said liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may comprise both erythrocytes as well as nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.

公开(公告)号：AT474228T

公开(公告)日：2010-07-15

申请号：AT07801434

申请日：2007-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： KOBOLD UWE ,  DUELFFER THOMAS ,  HERRMANN RUPERT ,  VOGT BERND ,  VON DER ELTZ HERBERT

IPC: G01N33/72 ,  C12Q1/37

Abstract: The present invention relates to a reagent for digestion of hemoglobin comprising a buffer, pepsin and a 1,3-dialkyl-imidazolium salt. It also discloses to the use of this reagent in a method for digesting hemoglobin, in a method for detecting HbA1c, and to a sampling tube for collection of a whole blood sample comprising said reagent for digesting hemoglobin.

公开(公告)号：HK1134341A1

公开(公告)日：2010-04-23

申请号：HK09111679

申请日：2009-12-11

Applicant: HOFFMANN LA ROCHE

Inventor： NASER WERNER ,  DUELFFER THOMAS ,  HERRMANN RUPERT ,  KOBOLD UWE ,  VON DER ELTZ HERBERT

IPC: G01N20060101

Abstract: The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.

公开(公告)号：AT452343T

公开(公告)日：2010-01-15

申请号：AT07725793

申请日：2007-06-04

Applicant: HOFFMANN LA ROCHE

Inventor： KOBOLD UWE ,  DUELFFER THOMAS ,  GROL MICHAEL ,  HERRMANN RUPERT ,  VON DER ELTZ HERBERT ,  VON PROFF LEOPOLD

IPC: G01N33/82

公开(公告)号：ES2347920T3

公开(公告)日：2010-11-25

申请号：ES07801434

申请日：2007-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： DUELFFER THOMAS ,  VON DER ELTZ HERBERT ,  KOBOLD UWE ,  VOGT BERND ,  HERRMANN RUPERT

IPC: G01N33/72 ,  C12Q1/37

Abstract: Método para la medición de HbA1c, comprendiendo el método las etapas siguientes: a) mezclar una muestra que contiene hemoglobina con un reactivo para la digestión de la hemoglobina, comprendiendo el reactivo: aa) un tampón, ab) pepsina, y ac) una sal 1,3-dialquil-imidazolio de un catión 1,3dialquil-imidazolio y un contraión. b) digerir la hemoglobina durante 1 a 60 minutos, obteniendo de esta manera un fragmento de hemoglobina que comprende los 14 aminoácidos N-terminales de HbA1c, y c) medir dicho fragmento N-terminal de HbA1c.

公开(公告)号：CA2649666A1

公开(公告)日：2007-12-13

申请号：CA2649666

申请日：2007-06-04

Applicant: HOFFMANN LA ROCHE

Inventor： KOBOLD UWE ,  VON DER ELTZ HERBERT ,  HERRMANN RUPERT ,  NASER WERNER ,  DUELFFER THOMAS

IPC: G01N33/50

Abstract: The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.