公开(公告)号：GB2545562A

公开(公告)日：2017-06-21

申请号：GB201620231

申请日：2016-11-29

Applicant: IBM

Inventor： WILLIAM THOMAS SPRATT ,  NING LI ,  DEVENDRA SADANA

IPC: G01N21/64

Abstract: A fluorescence detection system and method of use are disclosed. The system includes a light source 110 which emits excitation light; a sample unit 130 in which a sample is disposed; a first optical fiber 120 adapted to connect the light source to the sample unit; an avalanche photodiode array detector 140 which receives fluorescent light generated by the sample when the sample is irradiated with the excitation light; and a second optical fiber 150 adapted to connect the sample unit to the avalanche photodiode array detector (APD). The second optical fiber has a numerical aperture of equal to or greater than about 0.15 and is positioned such that its longitudinal axis is orthogonal to a longitudinal axis of the first optical fiber. A computer system including a processor and memory may also be included to receive and store sample information. The processor may select a photon-counting mode or a linear photomultiplier mode of operation for the detector. The sample unit may be a static unit, such as a cuvette, or a dynamic unit, such as a microfluidic channel.