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公开(公告)号：WO0184153A3
公开(公告)日：2002-07-11
申请号：PCT/US0111773
申请日：2001-04-11
Applicant: IDEXX LAB INC
Inventor： CLARK SCOTT M , SUVA ROBERT H , KEPRON MICHAEL R , BARSKI STANISLAW JR , WORKMAN ERWIN F JR
IPC: G01N33/566 , C12M1/00 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N33/558 , G01N37/00
CPC classification number: G01N33/558 , G01N33/54366 , Y10T436/11 , Y10T436/12 , Y10T436/255
Abstract: Devices and methods for performing assays to determine the presence or quantity of a specific analyte of interest in a fluid sample. In devices according to this invention two separate flow paths are established sequentially in the device with a single user activation step. The first flow path delivers the analyte of interest (if present in the sample) and conjugate soluble binding reagents to the solid phase. If analyte is present, an analyte:conjugate complex is formed and immobilized. The volume of sample delivered by this first path is determined by the absorbent capacity of the solid phase, and not by the amount of sample added to the device, relieving the user from the necessity of measuring the sample. The sample/conjugate mixture is prevented from entering the second flow path because the capillarity and the surface energy of the second flow path prevent it from being wetted by this mixture. The second flow path allows a wash reagent to remove unbound conjugate and sample from the solid phase to the absorbant, and optionally to deliver detection reagents.
Abstract translation: 用于进行测定以确定流体样品中感兴趣的特定分析物的存在或数量的装置和方法。 在根据本发明的设备中，在具有单个用户激活步骤的设备中依次建立两个单独的流路径。 第一流路传送感兴趣的分析物（如果存在于样品中）并将可溶性结合试剂与固相结合。 如果存在分析物，则形成并固定分析物：缀合物。 由该第一路径输送的样品的体积由固相的吸收能力确定，而不是通过添加到装置中的样品的量来确定，从而减轻了用户不必测量样品的量。 由于毛细管力和第二流路的表面能阻止其被该混合物润湿，因此防止样品/共轭物混合物进入第二流动路径。 第二流动路径允许洗涤剂将未结合的结合物和样品从固相除去到吸收剂，并且任选地递送检测试剂。
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公开(公告)号：CA2406724A1
公开(公告)日：2001-11-08
申请号：CA2406724
申请日：2001-04-11
Applicant: IDEXX LAB INC
Inventor： WORKMAN ERWIN F JR , BARSKI STANISLAW JR , CLARK SCOTT M , SUVA ROBERT H , KEPRON MICHAEL R
IPC: G01N33/566 , C12M1/00 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N33/558 , G01N37/00
Abstract: Devices and methods for performing assays to determine the presence or quantity of a specific analyte of interest in a fluid sample. In devices according to this invention two separate flow paths are established sequentially in the device with a single user activation step. The first flo w path delivers the analyte of interest (if present in the sample) and conjuga te soluble binding reagents to the solid phase. If analyte is present, an analyte:conjugate complex is formed and immobilized. The volume of sample delivered by this first path is determined by the absorbent capacity of the solid phase, and not by the amount of sample added to the device, relieving the user from the necessity of measuring the sample. The sample/conjugate mixture is prevented from entering the second flow path because the capillarity and the surface energy of the second flow path prevent it from being wetted by this mixture. The second flow path allows a wash reagent to remove unbound conjugate and sample from the solid phase to the absorbant, a nd optionally to deliver detection reagents.
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公开(公告)号：AU2001253354B2
公开(公告)日：2005-05-26
申请号：AU2001253354
申请日：2001-04-11
Applicant: IDEXX LAB INC
Inventor： BARSKI STANISLAW JR , KEPRON MICHAEL R , SUVA ROBERT H , CLARK SCOTT M , WORKMAN ERWIN F JR
IPC: G01N33/566 , C12M1/00 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N33/558 , G01N37/00 , G01N033/543
Abstract: Devices and methods for performing assays to determine the presence or quantity of a specific analyte of interest in a fluid sample. In devices according to this invention two separate flow paths are established sequentially in the device with a single user activation step. The first flow path delivers the analyte of interest (if present in the sample) and conjugate soluble binding reagents to the solid phase. If analyte is present, an analyte:conjugate complex is formed and immobilized. The volume of sample delivered by this first path is determined by the absorbent capacity of the solid phase, and not by the amount of sample added to the device, relieving the user from the necessity of measuring the sample. The sample/conjugate mixture is prevented from entering the second flow path because the capillarity and the surface energy of the second flow path prevent it from being wetted by this mixture. The second flow path allows a wash reagent to remove unbound conjugate and sample from the solid phase to the absorbant, and optionally to deliver detection reagents. The invention may be adapted to many assay formats including, sandwich immunoassays, colloidal gold, or sol particle assays, heterogeneous generic capture assays and competitive assays. In one embodiment, sandwich assays can be performed by immobilizing an analyte binding reagent on the solid phase, and drying a labeled analyte binding reagent in the first flow path. In a competitive assay embodiment, the first flow path would contain labeled analyte that is dissolved by the sample, and the analyte binding reagent is immobilized on the solid phase. In each of these embodiments, the assay can be further modified to run in a "generic capture" format, where the solid phase binding reagent is instead conjugated to a generic ligand such as biotin, and dried in the first flow path (either together or separately from the other assay reagents), and a generic ligand binding reagent (such as avidin) is immobilized on the solid phase. Another aspect of the present invention includes a subassembly for the immunoassay device that is comprised of a plastic housing and a means for delivering fluid and/or wash solution. This subassembly comprises a structure formed from a hydrophobic polymer selectively treated with a water insoluble surface active agent that has been applied as a solution in an organic solvent rendering portions of the surface hydrophilic. When the surface is contacted with an aqueous liquid, it flows only along the treated areas, creating a defined fluid flow path, thereby delivering sample/conjugate solutions to said solid phase.
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Patent Agency Ranking
公开(公告)号：AU5335401A
公开(公告)日：2001-11-12
申请号：AU5335401
申请日：2001-04-11
Applicant: IDEXX LAB INC
Inventor： CLARK SCOTT M , SUVA ROBERT H , KEPRON MICHAEL R , BARSKI STANISLAW JR , WORKMAN ERWIN F JR
IPC: G01N33/566 , C12M1/00 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N33/558 , G01N37/00
Abstract: Devices and methods for performing assays to determine the presence or quantity of a specific analyte of interest in a fluid sample. In devices according to this invention two separate flow paths are established sequentially in the device with a single user activation step. The first flow path delivers the analyte of interest (if present in the sample) and conjugate soluble binding reagents to the solid phase. If analyte is present, an analyte:conjugate complex is formed and immobilized. The volume of sample delivered by this first path is determined by the absorbent capacity of the solid phase, and not by the amount of sample added to the device, relieving the user from the necessity of measuring the sample. The sample/conjugate mixture is prevented from entering the second flow path because the capillarity and the surface energy of the second flow path prevent it from being wetted by this mixture. The second flow path allows a wash reagent to remove unbound conjugate and sample from the solid phase to the absorbant, and optionally to deliver detection reagents. The invention may be adapted to many assay formats including, sandwich immunoassays, colloidal gold, or sol particle assays, heterogeneous generic capture assays and competitive assays. In one embodiment, sandwich assays can be performed by immobilizing an analyte binding reagent on the solid phase, and drying a labeled analyte binding reagent in the first flow path. In a competitive assay embodiment, the first flow path would contain labeled analyte that is dissolved by the sample, and the analyte binding reagent is immobilized on the solid phase. In each of these embodiments, the assay can be further modified to run in a "generic capture" format, where the solid phase binding reagent is instead conjugated to a generic ligand such as biotin, and dried in the first flow path (either together or separately from the other assay reagents), and a generic ligand binding reagent (such as avidin) is immobilized on the solid phase. Another aspect of the present invention includes a subassembly for the immunoassay device that is comprised of a plastic housing and a means for delivering fluid and/or wash solution. This subassembly comprises a structure formed from a hydrophobic polymer selectively treated with a water insoluble surface active agent that has been applied as a solution in an organic solvent rendering portions of the surface hydrophilic. When the surface is contacted with an aqueous liquid, it flows only along the treated areas, creating a defined fluid flow path, thereby delivering sample/conjugate solutions to said solid phase.
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公开(公告)号：CA2232714A1
公开(公告)日：1997-04-03
申请号：CA2232714
申请日：1996-09-27
Applicant: IDEXX LAB INC
Inventor： PIERSON MARK W , GU HAOYI , WORKMAN ERWIN F JR , NAQUI ALI
Abstract: A self-contained incubator for growth of microorganism kit and methods for use of such a kit are provided. The kit and methods may detect the presence of microorganisms and may utilize a microorganism growth and indicator medium provided in a sample container along with a heat source, preferably generating heat through chemical means, and optionally heat shields, allowing for on-site testing of a microorganism present in a sample. The sample container may also include a removable vessel cap that includes a barrier separating the sample from a material capable of disinfecting the sample, thereby preventing contact of the sample and the material for a desired time period. The vessel cap may also be used independently in other applications.
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公开(公告)号：AU2001253354B9
公开(公告)日：2005-11-03
申请号：AU2001253354
申请日：2001-04-11
Applicant: IDEXX LAB INC
Inventor： BARSKI STANISLAW JR , KEPRON MICHAEL R , CLARK SCOTT M , WORKMAN ERWIN F JR , SUVA ROBERT H
IPC: G01N33/566 , C12M1/00 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N33/558 , G01N37/00 , G01N033/543
Abstract: Devices and methods for performing assays to determine the presence or quantity of a specific analyte of interest in a fluid sample. In devices according to this invention two separate flow paths are established sequentially in the device with a single user activation step. The first flow path delivers the analyte of interest (if present in the sample) and conjugate soluble binding reagents to the solid phase. If analyte is present, an analyte:conjugate complex is formed and immobilized. The volume of sample delivered by this first path is determined by the absorbent capacity of the solid phase, and not by the amount of sample added to the device, relieving the user from the necessity of measuring the sample. The sample/conjugate mixture is prevented from entering the second flow path because the capillarity and the surface energy of the second flow path prevent it from being wetted by this mixture. The second flow path allows a wash reagent to remove unbound conjugate and sample from the solid phase to the absorbant, and optionally to deliver detection reagents. The invention may be adapted to many assay formats including, sandwich immunoassays, colloidal gold, or sol particle assays, heterogeneous generic capture assays and competitive assays. In one embodiment, sandwich assays can be performed by immobilizing an analyte binding reagent on the solid phase, and drying a labeled analyte binding reagent in the first flow path. In a competitive assay embodiment, the first flow path would contain labeled analyte that is dissolved by the sample, and the analyte binding reagent is immobilized on the solid phase. In each of these embodiments, the assay can be further modified to run in a "generic capture" format, where the solid phase binding reagent is instead conjugated to a generic ligand such as biotin, and dried in the first flow path (either together or separately from the other assay reagents), and a generic ligand binding reagent (such as avidin) is immobilized on the solid phase. Another aspect of the present invention includes a subassembly for the immunoassay device that is comprised of a plastic housing and a means for delivering fluid and/or wash solution. This subassembly comprises a structure formed from a hydrophobic polymer selectively treated with a water insoluble surface active agent that has been applied as a solution in an organic solvent rendering portions of the surface hydrophilic. When the surface is contacted with an aqueous liquid, it flows only along the treated areas, creating a defined fluid flow path, thereby delivering sample/conjugate solutions to said solid phase.
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公开(公告)号：AU2001253354B8
公开(公告)日：2005-09-22
申请号：AU2001253354
申请日：2001-04-11
Applicant: IDEXX LAB INC
Inventor： BARSKI STANISLAW JR , KEPRON MICHAEL R , SUVA ROBERT H , CLARK SCOTT M , WORKMAN ERWIN F JR
IPC: G01N33/566 , C12M1/00 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N33/558 , G01N37/00 , G01N033/543
Abstract: Devices and methods for performing assays to determine the presence or quantity of a specific analyte of interest in a fluid sample. In devices according to this invention two separate flow paths are established sequentially in the device with a single user activation step. The first flow path delivers the analyte of interest (if present in the sample) and conjugate soluble binding reagents to the solid phase. If analyte is present, an analyte:conjugate complex is formed and immobilized. The volume of sample delivered by this first path is determined by the absorbent capacity of the solid phase, and not by the amount of sample added to the device, relieving the user from the necessity of measuring the sample. The sample/conjugate mixture is prevented from entering the second flow path because the capillarity and the surface energy of the second flow path prevent it from being wetted by this mixture. The second flow path allows a wash reagent to remove unbound conjugate and sample from the solid phase to the absorbant, and optionally to deliver detection reagents. The invention may be adapted to many assay formats including, sandwich immunoassays, colloidal gold, or sol particle assays, heterogeneous generic capture assays and competitive assays. In one embodiment, sandwich assays can be performed by immobilizing an analyte binding reagent on the solid phase, and drying a labeled analyte binding reagent in the first flow path. In a competitive assay embodiment, the first flow path would contain labeled analyte that is dissolved by the sample, and the analyte binding reagent is immobilized on the solid phase. In each of these embodiments, the assay can be further modified to run in a "generic capture" format, where the solid phase binding reagent is instead conjugated to a generic ligand such as biotin, and dried in the first flow path (either together or separately from the other assay reagents), and a generic ligand binding reagent (such as avidin) is immobilized on the solid phase. Another aspect of the present invention includes a subassembly for the immunoassay device that is comprised of a plastic housing and a means for delivering fluid and/or wash solution. This subassembly comprises a structure formed from a hydrophobic polymer selectively treated with a water insoluble surface active agent that has been applied as a solution in an organic solvent rendering portions of the surface hydrophilic. When the surface is contacted with an aqueous liquid, it flows only along the treated areas, creating a defined fluid flow path, thereby delivering sample/conjugate solutions to said solid phase.
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公开(公告)号：AT197717T
公开(公告)日：2000-12-15
申请号：AT96932350
申请日：1996-09-27
Applicant: IDEXX LAB INC
Inventor： NAQUI ALI , WORKMAN ERWIN F JR , GU HAOYI , PIERSON MARK W
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