公开(公告)号：EP0418940B1

公开(公告)日：1997-05-28

申请号：EP90202129.4

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： D'Alterio, Maurizio ,  Frontini, Dario

IPC: C12N9/96 ,  C12N9/04

CPC classification number: C12N9/96

公开(公告)号：EP0418940A1

公开(公告)日：1991-03-27

申请号：EP90202129.4

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： D'Alterio, Maurizio ,  Frontini, Dario

IPC: C12N9/96 ,  C12N9/04

CPC classification number: C12N9/96

Abstract: The stabilization of glucose oxidase in liquid reagent compositions for laboratory use is attained by having a phosphate buffer in a concentration of 400 to 3500 mM per liter of the liquid reagent. Such phosphate concentration exceeds by a least 100% that normally used to buffer the pH value.

Abstract translation: 用于实验室用途的液体试剂组合物中葡萄糖氧化酶的稳定是通过使用浓度为每升液体试剂400至3500mM的磷酸盐缓冲液来实现的。 这种磷酸盐浓度超过通常用于缓冲pH值的至少100％。

公开(公告)号：EP0415471A2

公开(公告)日：1991-03-06

申请号：EP90202128.6

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Frontini, Dario ,  D'Alterio, Maurizio

IPC: C12Q1/28

CPC classification number: C12Q1/28

Abstract: Spontaneous aspecific coloration in so-called Trinder reagents which can alter subsequent colorimetric determinations is inhibited by adding compounds of the chelating agent class to the solution. The resultant compositions contain a peroxidase enzyme, a phenylpyrazone derivative, a compound of phenolic or aromatic amine structure and a stabilizer. Preferred stablizers are ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA).

Abstract translation: 所谓Trinder试剂中的自发的特异性着色可通过向溶液中加入螯合剂类的化合物来抑制随后的比色测定。 所得组合物含有过氧化物酶，苯基腙衍生物，酚或芳族胺结构的化合物和稳定剂。 优选的稳定剂是乙二胺四乙酸（EDTA）和二亚乙基三胺五乙酸（DTPA）。

公开(公告)号：EP0415471B1

公开(公告)日：1994-11-09

申请号：EP90202128.6

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Frontini, Dario ,  D'Alterio, Maurizio

IPC: C12Q1/28

CPC classification number: C12Q1/28

公开(公告)号：EP0415471A3

公开(公告)日：1991-05-29

申请号：EP90202128.6

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Frontini, Dario ,  D'Alterio, Maurizio

IPC: C12Q1/28

CPC classification number: C12Q1/28

Abstract: Spontaneous aspecific coloration in so-called Trinder reagents which can alter subsequent colorimetric determinations is inhibited by adding compounds of the chelating agent class to the solution. The resultant compositions contain a peroxidase enzyme, a phenylpyrazone derivative, a compound of phenolic or aromatic amine structure and a stabilizer. Preferred stablizers are ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA).