公开(公告)号：EP0418940A1

公开(公告)日：1991-03-27

申请号：EP90202129.4

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： D'Alterio, Maurizio ,  Frontini, Dario

IPC: C12N9/96 ,  C12N9/04

CPC classification number: C12N9/96

Abstract: The stabilization of glucose oxidase in liquid reagent compositions for laboratory use is attained by having a phosphate buffer in a concentration of 400 to 3500 mM per liter of the liquid reagent. Such phosphate concentration exceeds by a least 100% that normally used to buffer the pH value.

Abstract translation: 用于实验室用途的液体试剂组合物中葡萄糖氧化酶的稳定是通过使用浓度为每升液体试剂400至3500mM的磷酸盐缓冲液来实现的。 这种磷酸盐浓度超过通常用于缓冲pH值的至少100％。

公开(公告)号：EP0415471A2

公开(公告)日：1991-03-06

申请号：EP90202128.6

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Frontini, Dario ,  D'Alterio, Maurizio

IPC: C12Q1/28

CPC classification number: C12Q1/28

Abstract: Spontaneous aspecific coloration in so-called Trinder reagents which can alter subsequent colorimetric determinations is inhibited by adding compounds of the chelating agent class to the solution. The resultant compositions contain a peroxidase enzyme, a phenylpyrazone derivative, a compound of phenolic or aromatic amine structure and a stabilizer. Preferred stablizers are ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA).

Abstract translation: 所谓Trinder试剂中的自发的特异性着色可通过向溶液中加入螯合剂类的化合物来抑制随后的比色测定。 所得组合物含有过氧化物酶，苯基腙衍生物，酚或芳族胺结构的化合物和稳定剂。 优选的稳定剂是乙二胺四乙酸（EDTA）和二亚乙基三胺五乙酸（DTPA）。

公开(公告)号：EP0872726A1

公开(公告)日：1998-10-21

申请号：EP97830175.2

申请日：1997-04-15

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Manzoni, Angelo ,  Daglio, Roberto ,  Frontini, Dario

IPC: G01N27/00 ,  G01N33/49 ,  G01N33/96 ,  G01N33/00

CPC classification number: G01N33/4925 ,  G01N33/0006 ,  G01N33/96 ,  G01N2496/70 ,  Y10T436/102499

Abstract: The present invention relates to a method for calibrating an instrument for the analysis of blood gases which possesses ion-sensitive electrodes and gas-sensitive electrodes and is used for the determination, in a biological fluid, of the concentration of electrolytes and metabolites in solution, and of the pH, pO 2 and pCO 2 , the said calibration being carried out with at least one buffered solution containing O 2 , a known or tonometer-measured amount of CO 2 and known concentrations of the electrolytes and metabolites to be determined, in which calibration the pO 2 titre is determined beforehand using atmospheric oxygen as reference.

Abstract translation: 本发明涉及一种用于校准的方法在溶液中具有仪器哪个离子敏感电极和气敏电极和用于测定血液气体的分析，在生物流体，电解质和代谢物的浓度， 和pH值，氧分压和二氧化碳分压的，所述的校准被执行与至少一个缓冲溶液包含O 2，公知的或CO 2的眼压计测量的量和已知的电解质和代谢物的浓度是确定的开采在哪个校准氧分压 滴度确定性开采预先使用大气中的氧作为参考。

公开(公告)号：EP0418940B1

公开(公告)日：1997-05-28

申请号：EP90202129.4

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： D'Alterio, Maurizio ,  Frontini, Dario

IPC: C12N9/96 ,  C12N9/04

CPC classification number: C12N9/96

公开(公告)号：EP0415471B1

公开(公告)日：1994-11-09

申请号：EP90202128.6

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Frontini, Dario ,  D'Alterio, Maurizio

IPC: C12Q1/28

CPC classification number: C12Q1/28

公开(公告)号：EP0415471A3

公开(公告)日：1991-05-29

申请号：EP90202128.6

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Frontini, Dario ,  D'Alterio, Maurizio

IPC: C12Q1/28

CPC classification number: C12Q1/28

Abstract: Spontaneous aspecific coloration in so-called Trinder reagents which can alter subsequent colorimetric determinations is inhibited by adding compounds of the chelating agent class to the solution. The resultant compositions contain a peroxidase enzyme, a phenylpyrazone derivative, a compound of phenolic or aromatic amine structure and a stabilizer. Preferred stablizers are ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA).