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公开(公告)号：EP0299660A3
公开(公告)日：1990-09-12
申请号：EP88306088.1
申请日：1988-07-04
Applicant: INSTRUMENTATION LABORATORY S.p.A.
Inventor： Bergkuist, Carolyn , Geiselman, Theodore S , Fraticelli, Yvonne
CPC classification number: C12Q1/005 , C12M21/18 , C12Q1/001 , Y10T436/117497
Abstract: A system for analyzing a biological fluid or the like for a constituent of interest comprises structure defining a sample inlet port, structure defining an analysis region, a measuring system connected in sensing relation to the analysis region, and structure defining a reaction chamber that has an immobilized enzyme capable of modifying the constituent of interest. Control means operates liquid flow means in a unidirectional sample flow mode to initially flow a sample of material to be analyzed from the sample inlet port to the reaction chamber and to the analysis region for a measurement of unmodified sample material, and then operates the liquid flow means in a bidirectional sample flow mode to oscillate the sample material in the reaction chamber and facilitate modification by the immobilized enzyme of the constituent of material in the sample. Modified sample material is then introduced into the analysis region and the measuring system is operated to obtain a measurement of modified sample material, the measurements of modified and unmodified sample material then being used to provide an indication of the amount of the constituent of interest in the sample material.
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公开(公告)号：EP0299660A2
公开(公告)日：1989-01-18
申请号：EP88306088.1
申请日：1988-07-04
Applicant: INSTRUMENTATION LABORATORY S.p.A.
Inventor： Bergkuist, Carolyn , Geiselman, Theodore S , Fraticelli, Yvonne
CPC classification number: C12Q1/005 , C12M21/18 , C12Q1/001 , Y10T436/117497
Abstract: A system for analyzing a biological fluid or the like for a constituent of interest comprises structure defining a sample inlet port, structure defining an analysis region, a measuring system connected in sensing relation to the analysis region, and structure defining a reaction chamber that has an immobilized enzyme capable of modifying the constituent of interest. Control means operates liquid flow means in a unidirectional sample flow mode to initially flow a sample of material to be analyzed from the sample inlet port to the reaction chamber and to the analysis region for a measurement of unmodified sample material, and then operates the liquid flow means in a bidirectional sample flow mode to oscillate the sample material in the reaction chamber and facilitate modification by the immobilized enzyme of the constituent of material in the sample. Modified sample material is then introduced into the analysis region and the measuring system is operated to obtain a measurement of modified sample material, the measurements of modified and unmodified sample material then being used to provide an indication of the amount of the constituent of interest in the sample material.
Abstract translation: 用于分析感兴趣成分的生物流体等的系统包括定义样品入口端口，限定分析区域的结构，与分析区域感测关系连接的测量系统的结构以及限定反应室的结构，其具有 能够修饰感兴趣的成分的固定化酶。 控制装置以单向样品流动模式操作液体流动装置，以最初将待分析材料的样品从样品入口流入反应室和分析区域，以测量未修饰的样品材料，然后操作液体流 意味着在双向样品流动模式中振荡样品材料在反应室中，并促进固定化酶对样品中材料成分的修饰。 然后将修改的样品材料引入分析区域，并操作测量系统以获得修改的样品材料的测量值，然后修改和未修改的样品材料的测量结果用于提供所述组合物的量的指示 样品材料
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