公开(公告)号：EP0304151B1

公开(公告)日：1993-12-15

申请号：EP88306087.3

申请日：1988-07-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Musacchio, John ,  Premoli, Pietro ,  Manzoni, Angelo ,  Bergkuist, Carolyn

IPC: G01N33/84

CPC classification number: G01N33/84

公开(公告)号：EP0304151A3

公开(公告)日：1990-09-12

申请号：EP88306087.3

申请日：1988-07-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Musacchio, John ,  Premoli, Pietro ,  Manzoni, Angelo ,  Bergkuist, Carolyn

IPC: G01N33/84

CPC classification number: G01N33/84

Abstract: A method for determining the concentrations of total calcium and at least one monovalent ion in a sample includes the steps of mixing the sample with a diluent that has a pH within the range pH 6.5 to 7.0 and includes 2-amino-2-hydroxymethyl-1,3 propanediol phosphate and is free of the monovalent ion. An aliquot of the diluted sample is concurrently contacted with a calcium-specific ion selective electrode and an ion selective electrode specifically responsive to the monovalent ion, the response of the calcium-specific ion selective electrode is measured as an indication of the concentration of total calcium in the sample, and the response of the monovalent ion specific ion selective electrode is measured as an indication of the concentration of the monovalent ion in the sample.

公开(公告)号：EP0304151A2

公开(公告)日：1989-02-22

申请号：EP88306087.3

申请日：1988-07-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Musacchio, John ,  Premoli, Pietro ,  Manzoni, Angelo ,  Bergkuist, Carolyn

IPC: G01N33/84

CPC classification number: G01N33/84

Abstract: A method for determining the concentrations of total calcium and at least one monovalent ion in a sample includes the steps of mixing the sample with a diluent that has a pH within the range pH 6.5 to 7.0 and includes 2-amino-2-hydroxymethyl-1,3 propanediol phosphate and is free of the monovalent ion. An aliquot of the diluted sample is concurrently contacted with a calcium-specific ion selective electrode and an ion selective electrode specifically responsive to the monovalent ion, the response of the calcium-specific ion selective electrode is measured as an indication of the concentration of total calcium in the sample, and the response of the monovalent ion specific ion selective electrode is measured as an indication of the concentration of the monovalent ion in the sample.

Abstract translation: 用于确定样品中总钙和至少一种一价离子浓度的方法包括以下步骤：将样品与pH在6.5至7.0范围内的pH值的稀释剂混合并包括2-氨基-2-羟甲基-1 ，3丙二醇磷酸酯，不含一价离子。 稀释的样品的等分试样同时与钙离子选择性电极和对单价离子特异性响应的离子选择性电极同时接触，测量钙离子选择性电极的响应作为总钙浓度的指示 在样品中，并且测量一价离子特异性离子选择电极的响应作为样品中一价离子浓度的指示。

公开(公告)号：EP0299660A3

公开(公告)日：1990-09-12

申请号：EP88306088.1

申请日：1988-07-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Bergkuist, Carolyn ,  Geiselman, Theodore S ,  Fraticelli, Yvonne

IPC: C12Q1/00 ,  C12M1/40 ,  G01N35/08 ,  C12Q1/54

CPC classification number: C12Q1/005 ,  C12M21/18 ,  C12Q1/001 ,  Y10T436/117497

Abstract: A system for analyzing a biological fluid or the like for a constituent of interest comprises structure defining a sample inlet port, structure defining an analysis region, a measuring system connected in sensing relation to the analysis region, and structure defining a reaction chamber that has an immobilized enzyme capable of modifying the constituent of interest. Control means operates liquid flow means in a unidirectional sample flow mode to initially flow a sample of material to be analyzed from the sample inlet port to the reaction chamber and to the analysis region for a measurement of unmodified sample material, and then operates the liquid flow means in a bidirectional sample flow mode to oscillate the sample material in the reaction chamber and facilitate modification by the immobilized enzyme of the constituent of material in the sample. Modified sample material is then introduced into the analysis region and the measuring system is operated to obtain a measurement of modified sample material, the measurements of modified and unmodified sample material then being used to provide an indication of the amount of the constituent of interest in the sample material.

公开(公告)号：EP0299660A2

公开(公告)日：1989-01-18

申请号：EP88306088.1

申请日：1988-07-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Bergkuist, Carolyn ,  Geiselman, Theodore S ,  Fraticelli, Yvonne

IPC: C12Q1/00 ,  C12M1/40 ,  G01N35/08 ,  C12Q1/54

CPC classification number: C12Q1/005 ,  C12M21/18 ,  C12Q1/001 ,  Y10T436/117497

Abstract: A system for analyzing a biological fluid or the like for a constituent of interest comprises structure defining a sample inlet port, structure defining an analysis region, a measuring system connected in sensing relation to the analysis region, and structure defining a reaction chamber that has an immobilized enzyme capable of modifying the constituent of interest. Control means operates liquid flow means in a unidirectional sample flow mode to initially flow a sample of material to be analyzed from the sample inlet port to the reaction chamber and to the analysis region for a measurement of unmodified sample material, and then operates the liquid flow means in a bidirectional sample flow mode to oscillate the sample material in the reaction chamber and facilitate modification by the immobilized enzyme of the constituent of material in the sample. Modified sample material is then introduced into the analysis region and the measuring system is operated to obtain a measurement of modified sample material, the measurements of modified and unmodified sample material then being used to provide an indication of the amount of the constituent of interest in the sample material.

Abstract translation: 用于分析感兴趣成分的生物流体等的系统包括定义样品入口端口，限定分析区域的结构，与分析区域感测关系连接的测量系统的结构以及限定反应室的结构，其具有 能够修饰感兴趣的成分的固定化酶。 控制装置以单向样品流动模式操作液体流动装置，以最初将待分析材料的样品从样品入口流入反应室和分析区域，以测量未修饰的样品材料，然后操作液体流 意味着在双向样品流动模式中振荡样品材料在反应室中，并促进固定化酶对样品中材料成分的修饰。 然后将修改的样品材料引入分析区域，并操作测量系统以获得修改的样品材料的测量值，然后修改和未修改的样品材料的测量结果用于提供所述组合物的量的指示 样品材料