公开(公告)号：WO2006071946A3

公开(公告)日：2006-12-14

申请号：PCT/US2005047278

申请日：2005-12-28

Applicant: INTEL CORP ,  SU XING ,  SUN LEI ,  GEORGER JACQUE

Inventor： SU XING ,  SUN LEI ,  GEORGER JACQUE

IPC: G01N33/543 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/68

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01L3/5085 ,  B01L2300/0636 ,  B01L2300/0819 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/5438 ,  C12Q2565/601

Abstract: Apparatus and methods are disclosed for electrically active combinatorial-chemical (EACC) chips for biochemical analyte detection. An apparatus includes a substrate that has an array of regions defining multiple cells, wherein each of the cells includes a reaction cavity that contains multiple functional binding groups. A method of detecting an analyte providing the reaction cavity between a source and a drain or a pair of electrodes, applying a voltage and monitoring a parameter indicative of an analyte characteristic. A process of fabricating an EACC include bonding an analyte to the multiple functional binding groups of each reaction cavity, and forming an analyte sensing structure including the substrate.

Abstract translation: 公开了用于生化分析物检测的电活性组合化学（EACC）芯片的装置和方法。 一种装置包括具有限定多个细胞的区域阵列的基底，其中每个细胞包括含有多个功能性结合基团的反应腔。 一种检测在源极和漏极或一对电极之间提供反应腔的分析物的方法，施加电压并监测指示分析物特征的参数。 制备EACC的方法包括将分析物结合到每个反应腔的多个功能性结合基团，以及形成包括该基质的分析物感测结构。

公开(公告)号：WO2005090948A3

公开(公告)日：2006-02-16

申请号：PCT/US2004043878

申请日：2004-12-28

Applicant: INTEL CORP ,  SU XING ,  ZHANG JINGWU ,  SUN LEI ,  BERLIN ANDREW

Inventor： SU XING ,  ZHANG JINGWU ,  SUN LEI ,  BERLIN ANDREW

IPC: G01N21/65 ,  G01N33/543

CPC classification number: G01N33/54346 ,  G01N21/658

Abstract: Composite organic-inorganic nanoclusters (COINs) are provided that produce surface-enhanced Raman signals (SERS) when excited by a laser. The nanoclusters include metal particles and a Raman-active organic compound. The metal required for achieving a suitable SERS signal is inherent in the nanocluster and a wide variety of Raman-active organic compounds and combinations thereof can be incorporated into the nanocluster. In addition, polymeric microspheres containing the nanoclusters and methods of making them are also provided. The nanoclusters and microspheres can be used, for example, in assays for multiplex detection of biological molecules.

Abstract translation: 提供了复合有机 - 无机纳米团簇（COINs），当激光激发时产生表面增强拉曼信号（SERS）。 纳米团簇包括金属颗粒和拉曼活性有机化合物。 实现合适的SERS信号所需的金属在纳米簇中是固有的，并且可以将多种拉曼活性有机化合物及其组合掺入纳米团簇中。 此外，还提供了含有纳米簇的聚合物微球及其制备方法。 纳米团簇和微球可用于例如生物分子多重检测的测定。

公开(公告)号：WO2005030996A2

公开(公告)日：2005-04-07

申请号：PCT/US2004031289

申请日：2004-09-23

Applicant: INTEL CORP

Inventor： SU XING ,  KOO TAE-WOONG ,  BERLIN ANDREW ,  SUN LEI ,  SUNDARARAJAN NARAYANAN ,  YAMAKAWA MINEO

IPC: C12Q1/68 ,  G06K19/06

CPC classification number: B82Y10/00 ,  B82Y5/00 ,  C12Q1/6816 ,  G06K19/06028 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2565/1025

Abstract: The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.

Abstract translation: 本公开涉及用于生产和/或使用分子条形码的方法。 在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。 标签可以附加到骨干和/或分支结构。 条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。 可以通过标签的类型和位置区分不同的条形码。 在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。 标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。 在替代实施例中，条形码可以通过单体单元的聚合来制备。 可以通过各种成像方式，例如表面等离子体共振，荧光或拉曼光谱来检测结合条形码。

公开(公告)号：WO2005014860A2

公开(公告)日：2005-02-17

申请号：PCT/US2004002391

申请日：2004-01-28

Applicant: INTEL CORP

Inventor： SUN LEI ,  SU XING

IPC: C12Q1/68

CPC classification number: C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/682 ,  G01N21/658 ,  C12Q2565/632 ,  C12Q2565/518 ,  C12Q2531/125 ,  C12Q2563/179

Abstract: The present methods, compositions and systems are concerned with biomolecule 130 detection, identification and/or quantification by rolling circle amplification (RCA) and Raman detection. In particular embodiments of the invention, the RCA is exponential RCA or linear RCA. In some embodiments of the invention, the Raman detection is SERS or SERRS. The circular DNA template 150, 210, 310 to be amplified may comprise one or more polythymidine 320 residues, resulting in amplification products 170, 230, 250, 330, 410 containing multiple polyadenylate 340, 420 residues. The polyadenylates 340, 420 may be directly detected by Raman detection. Alternatively, one or more Raman labels may be incorporated into the amplification products 170, 230, 250, 330, 410 to facilitate Raman detection. Because of the amplification produced by LRCA or ERCA and the enhanced Raman signal produced by multiple polyadenylates 340, 420 and/or Raman labels, detection of single copy biomolecules 130 is feasible using the disclosed methods, compositions and/or systems.

Abstract translation: 本发明的方法，组合物和系统涉及生物分子130的检测，通过滚环扩增（RCA）和拉曼检测进行鉴定和/或定量。 在本发明的具体实施例中，RCA是指数RCA或线性RCA。 在本发明的一些实施例中，拉曼检测是SERS或SERRS。 待扩增的环状DNA模板150,210,310可包含一个或多个聚胸苷320残基，产生含有多个聚腺苷酸340,420残基的扩增产物170,230,250,330,410。 聚腺苷酸340,420可以通过拉曼检测直接检测。 或者，可以将一个或多个拉曼标记结合到扩增产物170,230,250,330,410中以促进拉曼检测。 由于LRCA或ERCA产生的扩增和多聚腺苷酸340,420和/或拉曼标记产生的增强拉曼信号，使用所公开的方法，组合物和/或系统检测单拷贝生物分子130是可行的。

公开(公告)号：WO2008085538A3

公开(公告)日：2008-11-20

申请号：PCT/US2007072298

申请日：2007-06-27

Applicant: INTEL CORP ,  ZHANG JINGWU ,  LI HANDONG ,  SUNDARARAJAN NARAYAN ,  SU XING ,  SUN LEI

Inventor： ZHANG JINGWU ,  LI HANDONG ,  SUNDARARAJAN NARAYAN ,  SU XING ,  SUN LEI

IPC: G01N21/66 ,  G01J3/44 ,  G01N21/956

CPC classification number: G01N33/585 ,  C12Q1/6816 ,  G01N21/658 ,  G01N33/54373 ,  C12Q2563/155 ,  C12Q2565/632

Abstract: The embodiments of the invention are directed to a SERS cluster comprising a capture particle that is at least partially surrounded by analyte molecules, wherein both the capture particle and the analyte molecules surrounding the capture particle are at least partially surrounded by enhancer particles, wherein a majority of the analyte molecules are either sandwiched between capture and enhancer particles or located between junctions of the enhancer particles. The embodiments of the invention also relate to methods of manufacturing and detecting the SERS cluster. The embodiments of the invention also relate to a SERS active particle comprising a tag molecule comprising a Raman active compound and a probe or a linker having a specific biochemical binding capability and to a method for detecting of a target molecule using a SERS active particle having a tag molecule comprising a Raman active compound and a probe or a linker.

Abstract translation: 本发明的实施方案涉及包含至少部分被分析物分子包围的捕获颗粒的SERS簇，其中捕获颗粒和围绕捕获颗粒的分析物分子至少部分被增强剂颗粒包围，其中大部分 的分析物分子夹在捕获和增强剂颗粒之间或位于增强剂颗粒的连接点之间。 本发明的实施例还涉及制造和检测SERS群集的方法。 本发明的实施方案还涉及包含标签分子的SERS活性颗粒，所述标签分子包含拉曼活性化合物和具有特定生物化学结合能力的探针或接头，并涉及使用SERS活性颗粒检测靶分子的方法，所述SERS活性颗粒具有 标签分子，其包含拉曼活性化合物和探针或接头。

公开(公告)号：WO2005066367A3

公开(公告)日：2007-03-01

申请号：PCT/US2004043364

申请日：2004-12-24

Applicant: INTEL CORP ,  SU XING ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO ,  BERLIN ANDREW ,  SUN LEI ,  ZHANG YUEGANG

Inventor： SU XING ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO ,  BERLIN ANDREW ,  SUN LEI ,  ZHANG YUEGANG

IPC: C01B31/02 ,  C12Q1/68 ,  D01F9/127 ,  H01L51/00 ,  H01L51/30

CPC classification number: B82Y40/00 ,  B82Y10/00 ,  B82Y30/00 ,  C01B32/162 ,  C01B2202/08 ,  C01B2202/36 ,  D01F9/127 ,  H01L51/0048 ,  H01L51/0052

Abstract: The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles (140, 230) to polymer (120, 210) molecules, attaching the polymer (120, 210) molecules to a substrate, removing the polymer (120, 210) molecules and producing carbon nanotubes on the catalyst nanoparticles (140, 230). The polymer (120, 210) molecules alignment techniques. The nanotube arrays can be attached to selected areas (110, 310) of the substrate. Within the selected areas (110, 310), the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.

Abstract translation: 本文公开的方法，装置和系统涉及碳纳米管的有序阵列。 在本发明的具体实施方案中，纳米管阵列通过包括将催化剂纳米颗粒（140,230）附着到聚合物（120,210）分子上的方法形成，将聚合物（120,210）分子附着到基底上，去除聚合物 120,210）分子并在催化剂纳米颗粒（140,230）上产生碳纳米管。 聚合物（120,210）分子对齐技术。 纳米管阵列可以附着到基板的选定区域（110,310）。 在所选择的区域（110,310）内，纳米管是非随机分布的。 本文公开的其它实施方案涉及包括连接到衬底的纳米管的有序阵列和包括通过所要求保护的方法产生的连接到衬底的碳纳米管的有序阵列的系统的装置。 在某些实施方案中，本文提供了通过将分子线连接到双链DNA分子来对齐分子线的方法。

公开(公告)号：WO2004085988A2

公开(公告)日：2004-10-07

申请号：PCT/US2004002989

申请日：2004-02-04

Applicant: INTEL CORP

Inventor： SU XING ,  SUN LEI ,  KOO TAE-WOONG ,  CHAN SELANA

IPC: G01J3/44 ,  G06F11/00 ,  G06F11/07 ,  G01N

CPC classification number: G01J3/44 ,  B82Y15/00 ,  C12Q1/6825 ,  G01N21/658

Abstract: The intensity of the signals from surface enhanced Raman spectroscopy is increased by using lithium chloride (122) as an enhancer to activate a metallic structure (212) used for surface enhanced Raman spectroscopy (116). The increased signal intensity allows surface enhanced Raman spectroscopy to be utilized to detect individual analytes (120) such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.

Abstract translation: 通过使用氯化锂（122）作为增强剂来激活用于表面增强拉曼光谱的金属结构（212），增强了来自表面增强拉曼光谱的信号的强度（116）。 增加的信号强度允许表面增强拉曼光谱法用于检测单个分析物（120），例如核苷酸，例如在DNA测序中，而不需要染料或放射性标记。

公开(公告)号：WO2008005899A2

公开(公告)日：2008-01-10

申请号：PCT/US2007072595

申请日：2007-06-29

Applicant: INTEL CORP ,  SUN LEI ,  KOO TAE WOONG ,  WANG LIMING

Inventor： SUN LEI ,  KOO TAE WOONG ,  WANG LIMING

CPC classification number: G01N21/658 ,  G01J3/36 ,  G01J3/44

Abstract: A device (and methods of using and manufacturing the device) that utilize a plurality of photomultipliers (PMT)s or a photodiodes coupled with a set of filters to collect Raman signal from samples. Also a method of detecting Raman signals includes receiving Raman signals from a sample utilizing a plurality of photomultiplier tubes (PMT)s or photodiodes, wherein at least one PMT or photodiode provides a different Raman signal than at least one other PMT or photodiode.

Abstract translation: 使用多个光电倍增管（PMT）或与一组滤波器耦合的光电二极管以从样品收集拉曼信号的装置（以及使用和制造该装置的方法）。 检测拉曼信号的方法还包括利用多个光电倍增管（PMT）或光电二极管从样品接收拉曼信号，其中至少一个PMT或光电二极管提供与至少一个其它PMT或光电二极管不同的拉曼信号。

公开(公告)号：WO2006023458A9

公开(公告)日：2006-04-06

申请号：PCT/US2005029019

申请日：2005-08-13

Applicant: INTEL CORP ,  SUNDARARAJAN NARAYAN ,  SUN LEI ,  SU XING ,  YAMAKAWA MINEO ,  JINGWU ZHANG ,  CHAN SELENA ,  BERLIN ANDREW ,  KOO TAE-WOONG ,  ROTH MARK ,  GAFEN PHIL

Inventor： SUNDARARAJAN NARAYAN ,  SUN LEI ,  SU XING ,  YAMAKAWA MINEO ,  JINGWU ZHANG ,  CHAN SELENA ,  BERLIN ANDREW ,  KOO TAE-WOONG ,  ROTH MARK ,  GAFEN PHIL

IPC: G01N33/68 ,  G01N21/65

CPC classification number: G01N33/6851 ,  G01N21/658 ,  G01N33/54373 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6848 ,  Y02A90/26

Abstract: Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modification states of proteins and peptides using Surface Enhanced Raman (SERS) and Raman spectroscopy. Applications of embodiments of the present invention include, for example, proteome wide modification profiling and analyses with applications in disease diognosis, prognosis and drug efficacy studies, emzymatic activity profiling and assays.

Abstract translation: 本发明的实施方案提供了使用表面增强拉曼（SERS）和拉曼光谱法检测，鉴定，区分和定量蛋白质和肽的修饰状态的装置和方法。 本发明的实施方案的应用包括例如蛋白质组广泛的修饰分析和分析，其应用于疾病预后，预后和药物功效研究，酶活性分析和测定。

公开(公告)号：WO2005066369A3

公开(公告)日：2006-02-02

申请号：PCT/US2004043633

申请日：2004-12-28

Applicant: INTEL CORP ,  BERLIN ANDREW ,  SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  SUN LEI ,  SUNDARARAJAN NARAYAN

Inventor： BERLIN ANDREW ,  SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  SUN LEI ,  SUNDARARAJAN NARAYAN

IPC: C12Q1/68 ,  G01N21/65

CPC classification number: G01N21/658 ,  C12Q1/6869 ,  C12Q2565/632 ,  C12Q2533/101

Abstract: The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.

Abstract translation: 本文公开的方法和装置可用于检测核苷酸，核苷和碱基并用于核酸序列测定。 该方法涉及使用表面增强拉曼光谱（SERS）或表面增强相干抗斯托克斯拉曼光谱（SECARS）检测核苷酸，核苷或碱基。 检测可以是在核酸聚合反应（例如核酸测序反应）期间检测脱氧核苷酸三磷酸的摄取的核酸测序反应的一部分。 合成的新生链的核酸序列和模板链的互补序列可以通过跟踪聚合反应期间核苷酸掺入的顺序来确定。 提供了通过从糖部分切割碱来增强核苷酸或核苷的SERS信号的方法。 此外，提供了用于检测单碱基重复的方法。