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    METHODS AND COMPOSITIONS FOR DETECTING NUCLEIC ACIDS USING SCANNING PROBE MICROSCOPY AND NANOCODES
    3.
    发明申请
    METHODS AND COMPOSITIONS FOR DETECTING NUCLEIC ACIDS USING SCANNING PROBE MICROSCOPY AND NANOCODES 审中-公开
    使用扫描探针显微镜和纳米孔探测核酸的方法和组合物

    公开(公告)号：WO2005066368A3

    公开(公告)日：2005-11-24

    申请号：PCT/US2004043632

    申请日：2004-12-28

    Applicant: INTEL CORP ,  BERLIN ANDREW ,  YAMAKAWA MINEO

    Inventor： BERLIN ANDREW ,  YAMAKAWA MINEO

    IPC: C12Q1/68 ,  G01Q60/10 ,  G01Q60/50

    CPC classification number: C12Q1/6874 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q2565/601 ,  C12Q2563/155 ,  C12Q2525/179

    Abstract: A method for determining a nucleotide sequence of a nucleic acid is provided that includes contacting the nucleic acid with a series of labeled oligonucleotides for binding to the nucleic acid, wherein each labeled oligonucleotide includes a known nucleotide sequence and a molecular nanocode. The nanocode of an isolated labeled oligonucleotides that binds to the nucleic acid is then detected using SPM. Nanocodes of the present invention in certain aspects include detectable features beyond the arrangement of tags that encode information about the barcoded object, which assist in detecting the tags that encode information about the barcoded object. The detectable features include structures of a nanocode or associated with a nanocode, referred to herein as detectable feature tags, for error checking/error-correction, encryption, and data reduction/compression.

    Abstract translation: 提供了用于确定核酸的核苷酸序列的方法，其包括使核酸与一系列标记的寡核苷酸接触以结合核酸，其中每个标记的寡核苷酸包括已知的核苷酸序列和分子纳代码。 然后使用SPM检测与核酸结合的分离的标记的寡核苷酸的纳代码。 在某些方面，本发明的纳代码包括除了编码关于条形码化对象的信息的标签布置之外的可检测特征，其有助于检测编码关于条形码化对象的信息的标签。 可检测特征包括用于错误检查/错误校正，加密和数据简化/压缩的纳代码的结构或与纳代码关联的结构，在本文中被称为可检测特征标签。

    A METHOD AND DEVICE FOR DETECTING SMALL NUMBERS OF MOLECULES USING SURFACE-ENHANCED COHERENT ANTI-STOKES RAMAN SPECTROSCOPY
    4.
    发明申请
    A METHOD AND DEVICE FOR DETECTING SMALL NUMBERS OF MOLECULES USING SURFACE-ENHANCED COHERENT ANTI-STOKES RAMAN SPECTROSCOPY 审中-公开
    一种利用表面增强相干抗反射拉曼光谱检测小分子数的方法和装置

    公开(公告)号：WO2005038419A8

    公开(公告)日：2005-06-30

    申请号：PCT/US2004034598

    申请日：2004-10-18

    Applicant: INTEL CORP

    Inventor： KOO TAE-WOONG ,  GERTH CHRISTOPHER ,  YAMAKAWA MINEO

    IPC: G01J3/44 ,  G01N21/65

    CPC classification number: G01N21/658 ,  G01J3/44 ,  G01N2021/653

    Abstract: The device and method disclosed herein concern detecting, identifying, and or quantifying analytes, such as nucleic acids, with high resolution and fast response times using surface enhanced coherent anti-Stokes Raman spectroscopy. In certain embodiments of the invention, a small number molecular sample of the analyte (210) such as a nucleotide, passes through a microfluidic channel, microchannel, or nanochannel (185) and sample cell (175) that contains Raman-active surfaces, and is detected by surface enhanced, coherent anti-Stokes Raman spectroscopy (SECARS). Other embodiments of the invention concern an apparatus for analyte detection.

    Abstract translation: 本文公开的装置和方法涉及使用表面增强的相干反斯托克斯拉曼光谱法检测，鉴定和定量分析物，例如具有高分辨率和快速响应时间的核酸。 在本发明的某些实施方案中，诸如核苷酸的分析物（210）的少量分子样品通过含有拉曼活性表面的微流体通道，微通道或纳米通道（185）和样品池（175），以及 通过表面增强，相干反斯托克斯拉曼光谱（SECARS）检测。 本发明的其它实施方案涉及用于分析物检测的装置。

    A METHOD AND DEVICE FOR DETECTING A SMALL NUMBER OF MOLECULES USING SURFACE-ENHANCED COHERENT ANTI-STOKES RAMAN SPECTROSCOPY
    5.
    发明申请
    A METHOD AND DEVICE FOR DETECTING A SMALL NUMBER OF MOLECULES USING SURFACE-ENHANCED COHERENT ANTI-STOKES RAMAN SPECTROSCOPY 审中-公开
    一种使用表面增强相干抗反射拉曼光谱检测小数量分子的方法和装置

    公开(公告)号：WO2005040742A3

    公开(公告)日：2007-04-26

    申请号：PCT/US2004033054

    申请日：2004-10-06

    Applicant: INTEL CORP

    Inventor： KOO TAE-WOONG ,  YAMAKAWA MINEO ,  GERTH CHRISTOPHER

    IPC: G01J3/44 ,  G01N21/65

    CPC classification number: G01J3/44 ,  G01N21/658 ,  G01N2021/653 ,  G01N2021/656

    Abstract: The device and method disclosed herein concern detecting, identifying, and or quantifying analytes, such as nucleic acids, with high resolution and fast response times using surface enhanced coherent anti-Stokes Raman spectroscopy. In certain embodiments of the invention, a small number molecular sample of the analyte 210 such as a nucleotide, passes through a microfluidic channel, microchannel, or nanochannel 185 and sample cell 175 that contains Raman-active surfaces, and is detected by surface enhanced, coherent anti-Stokes Raman spectroscopy (SECARS). Other embodiments of the invention concern an apparatus for analyte detection.

    Abstract translation: 本文公开的装置和方法涉及使用表面增强的相干反斯托克斯拉曼光谱法检测，鉴定和定量分析物，例如具有高分辨率和快速响应时间的核酸。 在本发明的某些实施方案中，分析物210（例如核苷酸）的少量分子样品通过含有拉曼活性表面的微流体通道，微通道或纳米通道185和样品池175，并且通过表面增强， 相干反斯托克斯拉曼光谱（SECARS）。 本发明的其它实施方案涉及用于分析物检测的装置。

    PROGRAMMABLE MOLECULAR BARCODES
    6.
    发明申请
    PROGRAMMABLE MOLECULAR BARCODES 审中-公开
    可编程分子杆

    公开(公告)号：WO2005030996A2

    公开(公告)日：2005-04-07

    申请号：PCT/US2004031289

    申请日：2004-09-23

    Applicant: INTEL CORP

    Inventor： SU XING ,  KOO TAE-WOONG ,  BERLIN ANDREW ,  SUN LEI ,  SUNDARARAJAN NARAYANAN ,  YAMAKAWA MINEO

    IPC: C12Q1/68 ,  G06K19/06

    CPC classification number: B82Y10/00 ,  B82Y5/00 ,  C12Q1/6816 ,  G06K19/06028 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2565/1025

    Abstract: The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.

    Abstract translation: 本公开涉及用于生产和/或使用分子条形码的方法。 在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。 标签可以附加到骨干和/或分支结构。 条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。 可以通过标签的类型和位置区分不同的条形码。 在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。 标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。 在替代实施例中，条形码可以通过单体单元的聚合来制备。 可以通过各种成像方式，例如表面等离子体共振，荧光或拉曼光谱来检测结合条形码。

    DETECTING MOLECULAR BINDING BY MONITORING FEEDBACK CONTROLLED CANTILEVER DEFLECTIONS
    7.
    发明申请
    DETECTING MOLECULAR BINDING BY MONITORING FEEDBACK CONTROLLED CANTILEVER DEFLECTIONS 审中-公开
    通过监控反馈控制的CANTILEVER偏差检测分子结合

    公开(公告)号：WO2004029625A2

    公开(公告)日：2004-04-08

    申请号：PCT/US0330192

    申请日：2003-09-24

    Applicant: INTEL CORP

    Inventor： SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  YAMAKAWA MINEO ,  BERLIN ANDREW

    IPC: C12Q1/68 ,  G01N33/543 ,  G01B7/34

    CPC classification number: C12Q1/6825 ,  B82Y5/00 ,  G01N33/54373 ,  G01N2800/52 ,  Y10S977/924 ,  C12Q2565/501

    Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

    Abstract translation: 本方法和装置涉及使用探针分子检测和/或鉴定目标分析物。 在本发明的各种实施方案中，探针或分析物附着到一个或多个悬臂。 将探针与分析物结合导致由检测单元检测到的悬臂的偏转。 可以应用平衡力将悬臂恢复到其原始位置。 平衡力可以是磁性的，电的或辐射的。 生成平衡力的检测单元和机构可以可操作地耦合到诸如计算机的信息处理和控制单元。 计算机可以通过平衡偏转力和平衡力来调节将悬臂维持在固定位置的反馈回路。 样品中分析物的浓度可以从将悬臂维持在固定位置所需的平衡力的大小来确定。

    9.
    发明专利
    未知

    公开(公告)号：AT453853T

    公开(公告)日：2010-01-15

    申请号：AT03774634

    申请日：2003-10-07

    Applicant: INTEL CORP

    Inventor： CHAN SELENA ,  BERLIN ANDREW ,  KWON SUNGHOON ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO

    IPC: G01J3/44 ,  B01D67/00 ,  B01D71/02 ,  C12Q1/68 ,  G01N21/65

    Abstract: The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.

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