公开(公告)号：US20220282294A1

公开(公告)日：2022-09-08

申请号：US17631407

申请日：2020-07-31

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe ,  Kosuke Nakamoto ,  Hiroki Murase ,  Yasuaki Kimura

IPC: C12P19/34 ,  C07H19/10 ,  C07H19/20

Abstract: A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, R1 represents a decomposable protecting group, and R2 represents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.

公开(公告)号：US20220290232A1

公开(公告)日：2022-09-15

申请号：US17631418

申请日：2020-07-31

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe ,  Kosuke Nakamoto ,  Hiroki Murase ,  Yasuaki Kimura

IPC: C12Q1/6876

Abstract: A primer for amplifying a nucleic acid having a structure represented by formula (1): where A1 represents —S—, —S—S—, or —Se—, B represents a base, and R1 represents a decomposable protecting group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; Klenow fragment for making the 3′ ends blunt; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.