公开(公告)号：US20220282294A1

公开(公告)日：2022-09-08

申请号：US17631407

申请日：2020-07-31

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe ,  Kosuke Nakamoto ,  Hiroki Murase ,  Yasuaki Kimura

IPC: C12P19/34 ,  C07H19/10 ,  C07H19/20

Abstract: A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, R1 represents a decomposable protecting group, and R2 represents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.

公开(公告)号：US20150118713A1

公开(公告)日：2015-04-30

申请号：US14381681

申请日：2013-03-01

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroshi Abe ,  Yoshihiro Ito ,  Hideto Maruyama

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N15/1068 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2330/30

Abstract: A method for constructing a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands, wherein 2 or more fragments having at corresponding ends a functional group pair that can mutually couple through a chemical reaction are introduced into a cell, and a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands is formed by ligating mutually the fragments through a reaction between the functional groups in the cell.

Abstract translation: 构建包含1或2个核酸链的功能性核酸分子的方法，其中在相应末端具有可通过化学反应相互耦合的官能团对的2个或更多个片段被引入细胞中，并且功能性核酸分子 通过在细胞中的官能团之间的反应相互连接片段而形成包含1或2个核酸链。

公开(公告)号：US11597745B2

公开(公告)日：2023-03-07

申请号：US16977525

申请日：2019-03-08

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Yasuaki Kimura

IPC: C07H19/20 ,  C07H19/16

Abstract: A β-modified phosphoric acid compound precursor that inhibits the progress of a phosphorylation reaction having a partial structure represented by where A1 represents —SR1, —S—S—R1, —SeR1, or —X, where X is a halogen selected from fluoro, chloro, bromo, and iodo; R1 represents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; L1 represents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; L2 represents an alkyl group having 1 to 20 carbon atoms, or the like; L1 and L2 may be linked to each other to form a 4 to 6-membered ring structure; L1 and L2 may each have a substituent; and the symbol * represents a bond to be bonded to a phosphate group by phosphorylation. Further, provided are a reaction inhibitor and a medicine, each of which includes the β-modified phosphoric acid compound precursor.

公开(公告)号：US20250163098A1

公开(公告)日：2025-05-22

申请号：US18696694

申请日：2022-09-27

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Masahito Inagaki ,  Fumitaka Hashiya ,  Haruka Hiraoka ,  Naoko Abe

IPC: C07H21/04

Abstract: There is provided a nucleic acid strand cleaving method including a nucleic acid preparation step of preparing a nucleic acid to be cleaved having a structure represented by the following Formula (1), and a cleaving step of reacting the nucleic acid to be cleaved with a cleaving agent to cleave the nucleic acid to be cleaved at the part X of the Formula (1) to generate a nucleic acid having a structure represented by the following Formula (2). The cleaving agent is a metal nanoparticle containing an atom selected from the group consisting of silver, mercury, and cadmium. [Chemical Formula 1] Here, B represents a base, and X represents sulfur or selenium. NucA is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 5′ end side with reference to the X. NucB is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 3′ end side with reference to the X.

公开(公告)号：US20170283787A1

公开(公告)日：2017-10-05

申请号：US15505656

申请日：2015-08-26

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroshi Abe ,  Hideto MARUYAMA

IPC: C12N15/09 ,  C07H21/00 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12N15/09 ,  C07H1/00 ,  C07H21/00 ,  C12N2310/10 ,  C12N2310/141 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6811

Abstract: Provided, as a technique for binding a nucleic acid chain and a nucleic acid chain by a natural structure or an analogous structure thereto, is a non-enzymatic nucleic acid chain binding method, which is a method for binding a nucleic acid chain and a nucleic acid chain not via an enzymatic reaction, including a step of reacting a nucleic acid chain having a phosphorothioate group and a nucleic acid chain having a hydroxyl group or amino group in the presence of an electrophile.

公开(公告)号：US09598713B2

公开(公告)日：2017-03-21

申请号：US14381681

申请日：2013-03-01

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroshi Abe ,  Yoshihiro Ito ,  Hideto Maruyama

IPC: C12P19/34 ,  C12N15/10 ,  C12N15/113 ,  C12N15/11

CPC classification number: C12P19/34 ,  C12N15/1068 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2330/30

Abstract: A method for constructing a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands, wherein 2 or more fragments having at corresponding ends a functional group pair that can mutually couple through a chemical reaction are introduced into a cell, and a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands is formed by ligating mutually the fragments through a reaction between the functional groups in the cell.

公开(公告)号：US20230193294A1

公开(公告)日：2023-06-22

申请号：US17768942

申请日：2020-10-05

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe

IPC: C12N15/67 ,  C12P19/34 ,  C12P21/02

CPC classification number: C12N15/67 ,  C12P19/34 ,  C12P21/02

Abstract: A mRNA for the synthesis of a protein, including a translation region containing a start codon and a stop codon and an untranslated region positioned on the 5′-end side of the start codon, in which some phosphate groups within the range of at least from the 5′end of the untranslated region to 15 nt on the 3′-end side of the start codon are substituted with phosphorothioate groups, is provided. A method for producing an mRNA, including: a step of preparing a DNA template; a step of preparing an unmodified NTP containing ATP, GTP, CTP, and UTP, and a modified NTP in which at least one kind of phosphate group of the unmodified NTP is substituted with a phosphorothioate group; and a step of performing a transcription reaction with RNA polymerase using the DNA template as a template and the unmodified NTP and the modified NTP as substrates, is also provided.

公开(公告)号：US20210284679A1

公开(公告)日：2021-09-16

申请号：US16977525

申请日：2019-03-08

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Yasuaki Kimura

IPC: C07H19/20 ,  C07H19/16

Abstract: A β-modified phosphoric acid compound precursor having a partial structure represented by where A1 represents —SR1, —S—S—R1, —SeR1, or —X, where X is a halogen selected from fluorine, chlorine, bromine, and iodine; R1 represents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; L1 represents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; L2 represents an alkyl group having 1 to 20 carbon atoms, or the like; L1 and L2 may be linked to each other to form a 4 to 6-membered ring structure; L1 and L2 may each have a substituent; and the symbol * represents a bond to be bonded to a phosphate group by phosphorylation. Further, provided are a reaction inhibitor and a medicine, each of which includes the β-modified phosphoric acid compound precursor.

公开(公告)号：US10570385B2

公开(公告)日：2020-02-25

申请号：US15505656

申请日：2015-08-26

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Hiroshi Abe ,  Hideto Maruyama

IPC: C12N15/09 ,  C07H21/00 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6811 ,  C07H1/00 ,  C12Q1/68

Abstract: A non-enzymatic method is provided for binding a first nucleic acid chain to a second nucleic acid chain without introducing a sulfur atom into the combined nucleic acid chain, the method comprising reacting a first nucleic acid chain having a phosphorothioate group at the 3′ or 5′ terminus with a second nucleic acid chain having a hydroxyl group or an amino group at the 3′ or 5′ terminus in the presence of an electrophile that has a leaving group and is configured to leave the leaving group and bind to a sulfur atom of the phosphorothioate group of the first nucleic acid chain at the site to which the leaving group had been bound, and remove the sulfur atom from the phosphorothioate group of the first nucleic acid chain and a hydrogen atom from the hydroxyl group or from the amino group of the second nucleic acid chain via a nucleophilic substitution with an oxygen atom of the hydroxyl group or a nitrogen atom of the amino group of the second nucleic acid chain, and thereby form a bond between a phosphorus atom of the phosphate group of the first nucleic acid chain and the oxygen atom or the nitrogen atom of the second nucleic acid chain. Examples of structures produced by the binding method are shown below.

公开(公告)号：US20240327444A1

公开(公告)日：2024-10-03

申请号：US18576587

申请日：2022-07-05

Applicant: Japan Science and Technology Agency ,  National University Corporatjion Tokai National Higher Education and Research System

Inventor： Hiroshi Abe ,  Naoko Abe ,  Masahito Inagaki ,  Yasuaki Kimura ,  Fumitaka Hashiya ,  Zhenmin Li ,  Yuko Nakashima

IPC: C07H1/06 ,  A61K31/7105 ,  C07F9/24 ,  C07H17/02 ,  C07H21/00

CPC classification number: C07H1/06 ,  A61K31/7105 ,  C07F9/2458 ,  C07H17/02 ,  C07H21/00

Abstract: A method for purifying a nucleotide-based substance; including a protecting group introduction step of introducing a hydrophobic protecting group represented by the following formula (P1) or (P2) into a nucleotide-based substance to produce a hydrophobic nucleotide-based substance; an isolation and purification step of isolating and purifying the hydrophobic nucleotide-based substance under a hydrophobic environment; and a deprotection step of deprotecting the hydrophobic protecting group from the hydrophobic nucleotide-based substance to produce the nucleotide-based substance,






wherein R1 represents a linear or branched alkyl group having 1 to 30 carbon atoms, R4 represents hydrogen or a linear or branched alkyl group having 1 to 10 carbon atoms, R2, R3, R5 and R6 represent hydrogen, a linear or branched alkyl group having 1 to 10 carbon atoms, or the like, and may be the same or different; and * means a bond with a nucleotide-based substance.