公开(公告)号：US20220282294A1

公开(公告)日：2022-09-08

申请号：US17631407

申请日：2020-07-31

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe ,  Kosuke Nakamoto ,  Hiroki Murase ,  Yasuaki Kimura

IPC: C12P19/34 ,  C07H19/10 ,  C07H19/20

Abstract: A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, R1 represents a decomposable protecting group, and R2 represents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.

公开(公告)号：US20230193294A1

公开(公告)日：2023-06-22

申请号：US17768942

申请日：2020-10-05

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe

IPC: C12N15/67 ,  C12P19/34 ,  C12P21/02

CPC classification number: C12N15/67 ,  C12P19/34 ,  C12P21/02

Abstract: A mRNA for the synthesis of a protein, including a translation region containing a start codon and a stop codon and an untranslated region positioned on the 5′-end side of the start codon, in which some phosphate groups within the range of at least from the 5′end of the untranslated region to 15 nt on the 3′-end side of the start codon are substituted with phosphorothioate groups, is provided. A method for producing an mRNA, including: a step of preparing a DNA template; a step of preparing an unmodified NTP containing ATP, GTP, CTP, and UTP, and a modified NTP in which at least one kind of phosphate group of the unmodified NTP is substituted with a phosphorothioate group; and a step of performing a transcription reaction with RNA polymerase using the DNA template as a template and the unmodified NTP and the modified NTP as substrates, is also provided.

公开(公告)号：US20250163098A1

公开(公告)日：2025-05-22

申请号：US18696694

申请日：2022-09-27

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Masahito Inagaki ,  Fumitaka Hashiya ,  Haruka Hiraoka ,  Naoko Abe

IPC: C07H21/04

Abstract: There is provided a nucleic acid strand cleaving method including a nucleic acid preparation step of preparing a nucleic acid to be cleaved having a structure represented by the following Formula (1), and a cleaving step of reacting the nucleic acid to be cleaved with a cleaving agent to cleave the nucleic acid to be cleaved at the part X of the Formula (1) to generate a nucleic acid having a structure represented by the following Formula (2). The cleaving agent is a metal nanoparticle containing an atom selected from the group consisting of silver, mercury, and cadmium. [Chemical Formula 1] Here, B represents a base, and X represents sulfur or selenium. NucA is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 5′ end side with reference to the X. NucB is composed of at least one nucleotide and is a part of the nucleic acid to be cleaved, and represents a part on the 3′ end side with reference to the X.

公开(公告)号：US20240327444A1

公开(公告)日：2024-10-03

申请号：US18576587

申请日：2022-07-05

Applicant: Japan Science and Technology Agency ,  National University Corporatjion Tokai National Higher Education and Research System

Inventor： Hiroshi Abe ,  Naoko Abe ,  Masahito Inagaki ,  Yasuaki Kimura ,  Fumitaka Hashiya ,  Zhenmin Li ,  Yuko Nakashima

IPC: C07H1/06 ,  A61K31/7105 ,  C07F9/24 ,  C07H17/02 ,  C07H21/00

CPC classification number: C07H1/06 ,  A61K31/7105 ,  C07F9/2458 ,  C07H17/02 ,  C07H21/00

Abstract: A method for purifying a nucleotide-based substance; including a protecting group introduction step of introducing a hydrophobic protecting group represented by the following formula (P1) or (P2) into a nucleotide-based substance to produce a hydrophobic nucleotide-based substance; an isolation and purification step of isolating and purifying the hydrophobic nucleotide-based substance under a hydrophobic environment; and a deprotection step of deprotecting the hydrophobic protecting group from the hydrophobic nucleotide-based substance to produce the nucleotide-based substance,






wherein R1 represents a linear or branched alkyl group having 1 to 30 carbon atoms, R4 represents hydrogen or a linear or branched alkyl group having 1 to 10 carbon atoms, R2, R3, R5 and R6 represent hydrogen, a linear or branched alkyl group having 1 to 10 carbon atoms, or the like, and may be the same or different; and * means a bond with a nucleotide-based substance.

公开(公告)号：US20230097172A1

公开(公告)日：2023-03-30

申请号：US17802680

申请日：2021-02-19

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Yasuaki Kimura ,  Naoko Abe

IPC: C07H21/02 ,  C12P19/34

Abstract: A method for producing a capped RNA which is an RNA having the 5′-end modified with a cap, the method including: reacting an activated capping compound represented by with a monophosphate RNA having the 5′-end monophosphorylated, where, L represents a leaving group. The activated capping compound is preferably a compound represented by

公开(公告)号：US20220290232A1

公开(公告)日：2022-09-15

申请号：US17631418

申请日：2020-07-31

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe ,  Kosuke Nakamoto ,  Hiroki Murase ,  Yasuaki Kimura

IPC: C12Q1/6876

Abstract: A primer for amplifying a nucleic acid having a structure represented by formula (1): where A1 represents —S—, —S—S—, or —Se—, B represents a base, and R1 represents a decomposable protecting group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; Klenow fragment for making the 3′ ends blunt; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.