公开(公告)号：EP1029920A4

公开(公告)日：2002-12-18

申请号：EP98947770

申请日：1998-10-07

Applicant: KAO CORP

Inventor： TAKAIWA MIKIO ,  OKUDA MITSUYOSHI ,  SAEKI KATSUHISA ,  KUBOTA HIROMI ,  HITOMI JUN ,  KAGEYAMA YASUSHI ,  SHIKATA SHITSUW ,  NOMURA MASAFUMI

IPC: C11D3/386 ,  C12N1/21 ,  C12N9/54 ,  C12N15/57

CPC classification number: C12N9/54 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618

Abstract: An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same: (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, an activity 80 % or more as high as the one at the optimum pH value; (ii) when treated at 40 DEG C for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) being free from inhibition by oleic acid on the casein digesting activity. Because of being highly stable to various surfactants, being tolerant to fatty acids and showing a high stability to oxidizing agents, this alkaline protease is useful as an enzyme to be used in cleansers for automatic dish washers and a detergent for clothes, both containing bleaching components.

Abstract translation: 具有以下特性的碱性蛋白酶; 编码该基因的基因; 生产该微生物的微生物; 和含有它们的洗涤组合物：（i）在4至13的广泛pH值范围内起作用，并且在pH6至12下达到与最佳pH值相同的80％或更高的活性; （ii）当在40℃下处理30分钟时，在6至11的pH值范围内是稳定的; （iii）具有约8.9至9.1的等电点; 和（iv）不受油酸抑制酪蛋白消化活性。 由于对各种表面活性剂具有高度稳定性，对脂肪酸具有耐受性并且对氧化剂具有高度的稳定性，所以该碱性蛋白酶可用作用于自动洗碗机的清洁剂和用于衣服的洗涤剂的酶，两者都含有漂白组分 。

公开(公告)号：DE69833031T2

公开(公告)日：2006-08-24

申请号：DE69833031

申请日：1998-10-07

Applicant: KAO CORP

Inventor： TAKAIWA MIKIO ,  OKUDA MITSUYOSHI ,  SAEKI KATSUHISA ,  KUBOTA HIROMI ,  HITOMI JUN ,  KAGEYAMA YASUSHI ,  SHIKATA SHITSUW ,  NOMURA MASAFUMI

IPC: C12N15/57 ,  C11D3/386 ,  C12N1/21 ,  C12N9/54

Abstract: An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same: (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, an activity 80% or more as high as the one at the optimum pH value; (ii) when treated at 40 DEG C for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) being free from inhibition by oleic acid on the casein digesting activity. Because of being highly stable to various surfactants, being tolerant to fatty acids and showing a high stability to oxidizing agents, this alkaline protease is useful as an enzyme to be used in cleansers for automatic dish washers and a detergent for clothes, both containing bleaching components.

公开(公告)号：AU732369B2

公开(公告)日：2001-04-26

申请号：AU9457998

申请日：1998-10-07

Applicant: KAO CORP

Inventor： TAKAIWA MIKIO ,  OKUDA MITSUYOSHI ,  SAEKI KATSUHISA ,  KUBOTA HIROMI ,  KAGEYAMA YASUSHI ,  HITOMI JUN ,  SHIKATA SHITSUW ,  NOMURA MASAFUMI

IPC: C11D3/386 ,  C12N1/21 ,  C12N9/54 ,  C12N15/57

Abstract: An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same: (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, an activity 80% or more as high as the one at the optimum pH value; (ii) when treated at 40 DEG C for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) being free from inhibition by oleic acid on the casein digesting activity. Because of being highly stable to various surfactants, being tolerant to fatty acids and showing a high stability to oxidizing agents, this alkaline protease is useful as an enzyme to be used in cleansers for automatic dish washers and a detergent for clothes, both containing bleaching components.

公开(公告)号：DE10328887B4

公开(公告)日：2016-05-19

申请号：DE10328887

申请日：2003-06-26

Applicant: KAO CORP

Inventor： SATO TSUYOSHI ,  TAKIMURA YASUSHI ,  OKUDA MITSUYOSHI ,  SUMITOMO NOBUYUKI ,  NOMURA MASAFUMI ,  KOBAYASHI TOHRU

IPC: C12N9/54 ,  C07H21/04 ,  C12N15/57

Abstract: Alkalische Protease mit einer Präprosequenz, wobei die Präprosequenz eine mutierte Sequenz der Aminosäuresequenz von SEQ ID Nr. 1 darstellt oder eine Aminosäuresequenz mit einer mindestens 80%-igen, 87%-igen, 90%-igen, 95%-igen, 98%-igen oder höheren Homologie oder Identität mit der Aminosäuresequenz von SEQ ID Nr.: 1, in der zwei oder mehr Aminosäurereste ausgewählt aus (a) Position 52, (b) Position 75 und (c) Position 142 von SEQ ID Nr.: 1 oder zwei oder mehr Aminosäurereste ausgewählt aus diesen Positionen entsprechenden Positionen durch die folgenden Aminosäurereste ersetzt sind: in Position (a): Asparaginsäure oder Arginin, in Position (b): Alanin oder Arginin, und in Position (c): Lysin; wobei die alkalische Protease in reifer Form die Aminosäuresequenz von SEQ ID Nr. 2 aufweist oder eine Aminosäuresequenz mit einer mindestens 80%-igen, 87%-igen, 90%-igen, 95%-igen, 98%-igen oder höheren Homologie oder Identität mit dieser Aminosäuresequenz.

公开(公告)号：DK2284251T3

公开(公告)日：2014-08-25

申请号：DK10006498

申请日：2003-03-21

Applicant: KAO CORP

Inventor： OKUDA MITSUYOSHI ,  SATO TSUYOSHI ,  SAITO KAZUHIRO ,  SUMITOMO NOBUYUKI ,  IZAWA YOSHIFUMI ,  SAEKI KATSUHISA ,  KOBAYASHI TOHRU ,  NOMURA MASAFUMI

IPC: C11D3/386 ,  C12N1/21 ,  C12N5/10 ,  C12N9/16 ,  C12N9/54

公开(公告)号：DE60022111T2

公开(公告)日：2006-06-22

申请号：DE60022111

申请日：2000-03-16

Applicant: KAO CORP

Inventor： SHIKATA SHITSUW ,  NOMURA MASAFUMI ,  OKI TOSHIHIRO ,  TANIMOTO HITOSHI ,  TOKUMOTO TSUTOMU ,  OGURA NOBUYUKI

IPC: C11D3/386 ,  C11D3/02

Abstract: The present invention provides a detergent composition which is excellent in enzyme stability and exhibits excellent detergency particularly to protein-related dirt of socks and others even under laundering conditions at a lower temperature. That is, the present invention provides a detergent composition comprising specific proportions of (a) an anionic surfactant, (b) a chlorine scavenger, (c) a protease whose alpha -keratin-hydrolyzing activity at 10 DEG C is not less than 0.09x10 mu g/mPU.min and (d) a protease whose alpha -keratin-hydrolyzing activity at 10 DEG C is less than 0.09x10 mu g/mPU.min.

公开(公告)号：DK178139B1

公开(公告)日：2015-06-22

申请号：DKPA200300925

申请日：2003-06-20

Applicant: KAO CORP

Inventor： SATO TSUYOSHI ,  TAKIMURA YASUSHI ,  SUMITOMO NOBUYUKI ,  KOBAYASHI TOHRU ,  OKUDA MITSUYOSHI ,  NOMURA MASAFUMI

IPC: C12N15/57 ,  C07H21/04 ,  C11D3/386 ,  C12N9/54

Abstract: Den foreliggende opfindelse angår en alkalisk pratease med en præprosekvens, hvor præpro-sekvensen er en mutantsekvens af aminosyresekvensen i SEQ ID NO: 1, eller en aminosyresekvens med 80 % eller højere homologi med aminosyresekvensen i SEQ ID NO: 1, hvor aminosyrerester i (a) position 52, (b) position 75, og (c) position 142 i SEQ ID NO: 1, eller aminosyrerester i positioner svarende til disse positioner, substitueres med følgende aminosyrerester: i position (a): asparaginsyre eller arginin, i position (b): al an in eller arginin, og i position (c): lysin, og den alkaliske protease, når den er i moden form, har aminosyresekvensen i SEQ ID NO: 2 eller en aminosyresekvens med en homologi på 80 % eller højere med denne aminosyresekvens. Den foreliggende opfindelse angår for eksempel også et gen, der koder for den alkaliske protease. Ifølge den foreliggende opfindelse kan der fremstilles en alkalisk pratease i forøget mængde. Især kan der effektivt fremstilles en alkalisk pratease med aktivitet selv i nærvær af en fedtsyre i høj koncentration og med fremragende renseevne over for komplekse tilsmudsninger indeholdende proteiner og hudfedt.

公开(公告)号：DK1347044T3

公开(公告)日：2014-01-13

申请号：DK03006472

申请日：2003-03-21

Applicant: KAO CORP

Inventor： OKUDA MITSUYOSHI ,  SATO TSUYOSHI ,  SAITO KAZUHIRO ,  SUMITOMO NOBUYUKI ,  IZAWA YOSHIFUMI ,  SAEKI KATSUHISA ,  KOBAYASHI TOHRU ,  NOMURA MASAFUMI

IPC: C12N9/54 ,  C11D3/386 ,  C12N1/21 ,  C12N9/16

公开(公告)号：ES2243163T3

公开(公告)日：2005-12-01

申请号：ES00105328

申请日：2000-03-16

Applicant: KAO CORP

Inventor： SHIKATA SHITSUW ,  NOMURA MASAFUMI ,  OKI TOSHIHIRO ,  TANIMOTO HITOSHI ,  TOKUMOTO TSUTOMU ,  OGURA NOBUYUKI

IPC: C11D3/02 ,  C11D3/386

Abstract: Una composición detergente, que comprende: (a) de 15 a 40% en peso de un tensioactivo aniónico, (b) de 0, 5 a 5% en peso de un neutralizador químico del cloro, (c) una proteasa cuya actividad hidrolizante sobre la á-queratina a 10ºC es no menor que 0, 09 x 10-3 ìg/mPUumin, y (d) una proteasa cuya actividad hidrolizante sobre la á-queratina a 10ºC es menor que 0, 09 x 10-3 ìg/mPUumin, en la que (c)+(d) = de 0, 01 a 0, 5% en peso (como producto enzimático en polvo), (c)/(d) = de 1/5 a 5/1 y [(c)+(d)]/(b) = de 1/100 a 1/2 (relación de pesos como producto enzimático en polvo), y un éter alquílico o alquenílico de polioxialquileno cuyo balance hidrófilo-lipófilo (método de Griffin) es de 11, 5 a 17.

公开(公告)号：DE10328887A1

公开(公告)日：2004-04-29

申请号：DE10328887

申请日：2003-06-26

Applicant: KAO CORP

Inventor： SATO TSUYOSHI ,  TAKIMURA YASUSHI ,  OKUDA MITSUYOSHI ,  SUMITOMO NOBUYUKI ,  NOMURA MASAFUMI ,  KOBAYASHI TOHRU

IPC: C07H21/04 ,  C12N9/54 ,  C12N15/57

Abstract: The present invention relates to an alkaline protease having a prepro sequence, wherein the prepro sequence is a mutant sequence of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 80% or higher homology to the amino acid sequence of SEQ ID NO: 1, in which amino acid residues at (a) position 52, (b) position 75, and (c) position 142 of SEQ ID NO: 1, or amino acid residues at positions corresponding to these positions are substituted by the following amino acid residues: at position (a): aspartic acid or arginine, at position (b): alanine or arginine, and at position (c): lysine; and the alkaline protease, when in a mature form, has the amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having a homology of 80% or higher to this amino acid sequence. The present invention also relates to, for example, a gene encoding the alkaline protease. According to the present invention, an alkaline protease increased in production can be produced. In particular, there can be efficiently produced an alkaline protease having an activity even in the presence of a fatty acid of high concentration and exhibiting excellent detergency against complex soils containing proteins and sebum.