公开(公告)号：DE3588239T3

公开(公告)日：2007-03-08

申请号：DE3588239

申请日：1985-06-17

Applicant: KAUFFMAN STUART A

Inventor： BALLIVET MARC ,  KAUFFMAN STUART A

IPC: C12N15/11 ,  G01N33/50 ,  A61K38/02 ,  A61K39/00 ,  A61K39/29 ,  C07H21/02 ,  C07H21/04 ,  C07K2/00 ,  C07K14/02 ,  C07K14/485 ,  C12N1/21 ,  C12N9/38 ,  C12N15/00 ,  C12N15/09 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66 ,  C12N15/68 ,  C12N15/70 ,  C12P21/00 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/15 ,  G01N33/48 ,  G01N33/53 ,  G01N33/566 ,  G01N33/577

Abstract: Microbiologicale prodn. of peptides or polypeptides (I) comprises first producing, simultaneously in the same medium, many genes (A) composed at least partly of stochastic, synthetic polynucleotides, and introducing these genes into host cells. The (A)-contg. strains are cultured so as to clone (A) and induce expression of proteins coded by (A). The modified strains are screened/selected to identify those which produce the required (I), according to a particular property. These strains are then isolated and cultured to produce (I). In modification, the (A)-contg. transformants are selected for presence of a particular stochastic DNA or RNA sequence, and such sequences recovered from cultures of the appropriate strains.

公开(公告)号：DE3588239T2

公开(公告)日：2003-01-02

申请号：DE3588239

申请日：1985-06-17

Applicant: KAUFFMAN STUART A

Inventor： BALLIVET MARC ,  KAUFFMAN STUART A

IPC: G01N33/50 ,  A61K39/00 ,  A61K39/29 ,  C07H21/02 ,  C07H21/04 ,  C07K14/02 ,  C07K14/485 ,  C12N1/21 ,  C12N9/38 ,  C12N15/00 ,  C12N15/09 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66 ,  C12N15/68 ,  C12N15/70 ,  C12P21/00 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/15 ,  G01N33/48 ,  G01N33/53 ,  G01N33/566 ,  G01N33/577 ,  C12N15/11 ,  C07K2/00 ,  A61K38/02

Abstract: Microbiologicale prodn. of peptides or polypeptides (I) comprises first producing, simultaneously in the same medium, many genes (A) composed at least partly of stochastic, synthetic polynucleotides, and introducing these genes into host cells. The (A)-contg. strains are cultured so as to clone (A) and induce expression of proteins coded by (A). The modified strains are screened/selected to identify those which produce the required (I), according to a particular property. These strains are then isolated and cultured to produce (I). In modification, the (A)-contg. transformants are selected for presence of a particular stochastic DNA or RNA sequence, and such sequences recovered from cultures of the appropriate strains.

公开(公告)号：WO0004196A2

公开(公告)日：2000-01-27

申请号：PCT/US9915860

申请日：1999-07-13

Applicant: CISTEM MOLECULAR CORP ,  KAUFFMAN STUART A ,  BALLIVET MARC

Inventor： KAUFFMAN STUART A ,  BALLIVET MARC

IPC: C12N15/09 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C12Q1/6811 ,  C12N15/1034 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q2525/179 ,  C12Q2522/101 ,  C12Q2521/507

Abstract: The invention provides a method of identifying nucleic acid molecules that contain cis acting nucleic acid elements. Also provided is a method of isolating nucleic acid binding factors. The invention also provides methods of identifying compounds that are cis acting nucleic acid element analogs, compounds that are nucleic acid binding factor analogs, compounds that selectively bind cis acting nucleic acid elements and compounds that selectively displace binding between a nucleic acid binding factor and a cis acting nucleic acid element or between nucleic acid binding factors. Also provided is a method of determining a binding state of a nucleic acid. Pluralities of isolated nucleic acid molecules containing cis acting nucleic acid elements, of isolated cis acting nucleic acid elements and of isolated nucleic acid binding factors are also provided. The invention further provides methods of treating pathological conditions using molecules of the invention to alter genetic activites of nucleic acids involved in pathological conditions.

Abstract translation: 本发明提供了鉴定含有顺式作用核酸元件的核酸分子的方法。 还提供了分离核酸结合因子的方法。 本发明还提供鉴定作为顺式作用核酸元件类似物的化合物，作为核酸结合因子类似物的化合物，选择性结合顺式作用核酸元件的化合物和选择性取代核酸结合因子与顺式 作用核酸元件或核酸结合因子之间。 还提供了确定核酸的结合状态的方法。 还提供了含有顺式作用核酸元件，分离的顺式作用核酸元件和分离的核酸结合因子的多种分离的核酸分子。 本发明还提供使用本发明分子治疗病理状况以改变参与病理状况的核酸的遗传活性的方法。