公开(公告)号：KR100777973B1

公开(公告)日：2007-11-29

申请号：KR20060065828

申请日：2006-07-13

Applicant: KOREA RES INST OF STANDARDS

Inventor： YUN WAN SOO ,  PARK HYUNG JU ,  CHI YOUNG SHIK

IPC: G01N33/53 ,  G01N33/50

CPC classification number: G01N33/5438 ,  C12Q2565/607

Abstract: A bio-sensor is provided to detect the existence and the concentration of various bio-materials such as genes and proteins to be measured efficiently and electrically. A bio-sensor in which a plurality of interdigitated electrode sensor units, each of which is independently operated, is integrated in a substrate comprises a first and a second electrodes formed to be spaced apart from one another with a multi-line shape and a biomolecule receptor fixed on a sensor which is fixed on the substrate exposed between the first and second electrodes and specifically bound to biomolecules, and is characterized in that the biomolecule is analyzed by the number of the multi-line electrode sensor units electrically conducted by conductive particles directly bound to biomolecules captured by the biomolecule receptor or conductive particles bound to the biomolecules via the biomolecule receptor. A method for analyzing biomolecules comprises the steps of: (a) contacting a sample solution including the biomolecules to be measured with the bio-sensor to capture the biomolecules in the sensor-fixed biomolecule receptor fixed on the substrate exposed between the first and second electrodes of the multi-line electrode sensor units independently operated to one another; (b) contacting the biomolecules bound to the sensor-fixed biomolecules receptor with the particle fixed biomolecule receptor in which the conductive particles are fixed to bind the conductive particles to the biomolecules; (c) measuring the electric conductivity of the bio-sensor; and (d) converting the electric conductivity into the concentration of the biomolecules in the sample solution from the relation between the rate of the sensor showing the change of the electric conductivity before and after the sample solution contact and the concentration.

Abstract translation: 提供生物传感器来检测各种生物材料的存在和浓度，例如有效和电气地测量的基因和蛋白质。 一种生物传感器，其中多个独立操作的叉指电极传感器单元集成在基板中，包括形成为彼此间隔开的多线形状的第一和第二电极以及生物分子 受体固定在传感器上，该传感器固定在暴露在第一和第二电极之间并且特异性结合生物分子的基板上，其特征在于通过直接导电颗粒电导电的多线电极传感器单元的数量来分析生物分子 结合生物分子捕获的生物分子或通过生物分子受体结合生物分子的导电颗粒。 用于分析生物分子的方法包括以下步骤：（a）使包含待测量的生物分子的样品溶液与生物传感器接触，以捕获固定在暴露于第一和第二电极之间的衬底上的传感器固定的生物分子受体中的生物分子 的多线电极传感器单元彼此独立操作; （b）将与传感器固定的生物分子受体结合的生物分子与固定有导电颗粒的颗粒固定生物分子受体接触，以将导电颗粒结合到生物分子上; （c）测量生物传感器的电导率; 和（d）从示出溶液接触之前和之后的电导率的变化与浓度之间的关系，将样品溶液中的电导率转换成样品溶液中的生物分子的浓度。

公开(公告)号：WO2010110610A2

公开(公告)日：2010-09-30

申请号：PCT/KR2010001839

申请日：2010-03-25

Applicant: KOREA RES INST OF STANDARDS ,  YUN WAN SOO ,  PARK HYUNG JU ,  CHI YOUNG SHIK

Inventor： YUN WAN SOO ,  PARK HYUNG JU ,  CHI YOUNG SHIK

IPC: B82B1/00 ,  B82B3/00

CPC classification number: B82Y10/00 ,  B82Y30/00 ,  H01L29/0665 ,  H01L29/127 ,  H01L29/7613

Abstract: Provided is a nanogap device having metallic nanodots. To be specific, the nanogap device having a plurality of metallic nanodots performed by a heat-treatment process of the device having a large separation distance between electrodes formed by lithographic technology is provided for easy fabrication of a biosensor and a memory device having a superior performance. In particular, the nanogap device can be easily applied to the biosensor that has the high sensitivity in the detection of biomolecules and the suitability of statistical measurement method.

Abstract translation: 提供了具有金属纳米点的纳米间隙装置。 具体而言，提供具有多个金属纳米点的纳米间隙装置，所述纳米间隙装置通过光刻技术形成的电极之间具有大的间隔距离的装置的热处理工艺进行，以便于制造具有优异性能的生物传感器和存储装置 。 特别是，纳米间隙装置可以容易地应用于对生物分子检测具有高灵敏度和统计测量方法的适用性的生物传感器。