公开(公告)号：EP3264093B1

公开(公告)日：2019-09-04

申请号：EP16883939.7

申请日：2016-05-18

Applicant: Korea Institute of Science and Technology

Inventor： KANG, Ji Yoon ,  SHIN, Kyeong-Sik ,  JI, Jae-hoon ,  KIM, Tae Song ,  KIM, Youngsoo ,  KIM, Sangyun

IPC: G01N33/68 ,  G01N27/02

公开(公告)号：EP3264093A1

公开(公告)日：2018-01-03

申请号：EP16883939.7

申请日：2016-05-18

Applicant: Korea Institute of Science and Technology

Inventor： KANG, Ji Yoon ,  SHIN, Kyeong-Sik ,  JI, Jae-hoon ,  KIM, Tae Song ,  KIM, Youngsoo ,  KIM, Sangyun

IPC: G01N33/68 ,  G01N33/543 ,  G01N33/535 ,  G01N21/552 ,  G01N27/02

CPC classification number: G01N33/6896 ,  G01N27/02 ,  G01N27/026 ,  G01N2333/47 ,  G01N2333/4709 ,  G01N2800/28

Abstract: The present invention relates to a method for diagnosing a disease using an analysis of oligomer of an abnormal aggregated protein includes: (1) preparing a body fluid sample including at least one of blood, blood plasma, blood serum, saliva, urine, tear, and mucus; (2) making a dilution of the body fluid sample; (3) using a biosensor to measure and detect an aggregated protein in the diluted body fluid sample; (4) analyzing a signal change of the biosensor caused by the dilution of the aggregated protein to determine a slope according to the dilution from the measurements; and (5) analyzing a proportion of the oligomer from the slope according to the dilution to make a diagnosis. The method uses a biosensor to measure the impedance and the protein concentration of blood and detects the slope according to the numerical value of the monomer and the oligomer to diagnose normal or abnormal protein aggregation or the associated diseases with more accuracy.

Abstract translation: 本发明涉及使用异常凝聚蛋白质的低聚物分析来诊断疾病的方法，其包括：（1）制备包括血液，血浆，血清，唾液，尿液，泪液， 和粘液; （2）对体液样品进行稀释; （3）使用生物传感器测量和检测稀释体液样品中的聚集蛋白质; （4）分析由聚集蛋白质的稀释引起的生物传感器的信号变化，以根据测量结果的稀释度确定斜率; 和（5）根据稀释度从斜率分析低聚物的比例以作出诊断。 该方法使用生物传感器测量血液中的阻抗和蛋白质浓度，并根据单体和寡聚体的数值检测斜率，以更准确地诊断正常或异常蛋白质聚集或相关疾病。