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    SYSTEM FOR DETECTING BIOMOLECULE WI TH HIGH SENSITIVITY USING MICRO-CANT I LEVER
    1.
    发明申请
    SYSTEM FOR DETECTING BIOMOLECULE WI TH HIGH SENSITIVITY USING MICRO-CANT I LEVER 审中-公开
    使用微型灯检测生物分子的高灵敏度系统I LEVER

    公开(公告)号：WO2010055959A1

    公开(公告)日：2010-05-20

    申请号：PCT/KR2008/006638

    申请日：2008-11-11

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HWANG, Kyo Seon ,  KIM, Tae Song ,  KIM, Sang Kyung ,  LEE, Sang Myung ,  LEE, Yoon Sik

    Inventor： HWANG, Kyo Seon ,  KIM, Tae Song ,  KIM, Sang Kyung ,  LEE, Sang Myung ,  LEE, Yoon Sik

    IPC: G01N33/53

    CPC classification number: G01N33/54373

    Abstract: Provided is a protein detection system using a micro-cantilever and based on immune responses, wherein the micro-cantilever shows significantly improved sensitivity to allow detection of a trace amount of biomolecule. To the micro-cantilever, sandwich immunoassay is applied, and the sandwich immunoassay uses a polyclonal antibody or silica nanoparticles having a monoclonal antibody bound thereto, so that variations in the output signals of the cantilever are amplified and the detection sensitivity is significantly improved. The system enables detection of disease specific antigen at several femtomolar levels, and makes it possible to detect a trace amount of protein related to diseases, particularly to cancers, with ease.

    Abstract translation: 提供了使用微悬臂梁并基于免疫应答的蛋白质检测系统，其中微悬臂梁显示显着改善的灵敏度以允许检测微量的生物分子。 对微悬臂梁进行夹心免疫测定，夹心免疫测定使用结合有单克隆抗体的多克隆抗体或二氧化硅纳米粒子，扩大悬臂输出信号的变化，检测灵敏度明显提高。 该系统能够以几个摩尔体积水平检测疾病特异性抗原，并且可以容易地检测与疾病，特别是癌症相关的微量蛋白质。

    METHOD AND SYSTEM FOR DETECTING BIO-ELEMENT
    2.
    发明申请
    METHOD AND SYSTEM FOR DETECTING BIO-ELEMENT 审中-公开
    用于检测生物元素的方法和系统

    公开(公告)号：WO2006031072A1

    公开(公告)日：2006-03-23

    申请号：PCT/KR2005/003066

    申请日：2005-09-15

    Applicant: Korea Institute of Science and Technology ,  LEE, Jeong Hoon ,  KIM, Tae Song ,  YOON, Dae Sung ,  HWANG, Kyo Seon

    Inventor： LEE, Jeong Hoon ,  KIM, Tae Song ,  YOON, Dae Sung ,  HWANG, Kyo Seon

    IPC: G01N29/12

    CPC classification number: G01N29/022 ,  B82Y30/00 ,  B82Y35/00 ,  G01N29/036 ,  G01N29/326 ,  G01N2291/0215 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0258 ,  G01N2291/0426 ,  G01N2291/0427 ,  Y10T436/143333

    Abstract: Disclosed are system and method for measuring a bio-element capable of accurately detecting whether a bio-element such as protein, gene and the like is present in an atmosphere or vapor phase having controlled temperature and humidity thereof and measuring a content of the bio- element. According to an embodiment of the invention, the method comprises steps of preparing a cantilever sensor having a plurality of cantilevers; measuring a basis resonant frequency for the plurality of cantilevers; reacting the cantilevers with a sample including a bio-element; measuring resonant frequencies of the cantilevers after the reaction, in a closed system that is isolated from an exterior environment and temperature and humidity thereof are controlled to a specific state; and calculating variations of the resonant frequencies of the cantilevers before and after the reaction to carry out a quantitative analysis of the bio-element included in the sample.

    Abstract translation: 本发明公开了一种用于测量生物元素的系统和方法，其能够准确检测在具有受控温度和湿度的气氛或气相中是否存在诸如蛋白质，基因等的生物元件，并测量生物元素的含量， 元件。 根据本发明的实施例，该方法包括制备具有多个悬臂的悬臂传感器的步骤; 测量所述多个悬臂的基本共振频率; 使悬臂与包括生物元件的样品反应; 在与外部环境隔离的封闭系统中测量反应后的悬臂的谐振频率，并将其温度和湿度控制在特定状态; 并计算反应前后悬臂的共振频率的变化，以对样品中包含的生物元素进行定量分析。

    METHOD OF FABRICATING BLOCK-COPOLYMER-BASED 3D POLYMORPHIC ARTIFICIAL BILAYER MEMBRANE STRUCTURE
    4.
    发明公开
    METHOD OF FABRICATING BLOCK-COPOLYMER-BASED 3D POLYMORPHIC ARTIFICIAL BILAYER MEMBRANE STRUCTURE 审中-公开

    公开(公告)号：EP4241953A1

    公开(公告)日：2023-09-13

    申请号：EP22209605.9

    申请日：2022-11-25

    Applicant: Korea Institute of Science and Technology

    Inventor： KIM, Tae Song ,  KANG, Dong Hyun

    IPC: B29C33/42

    Abstract: The present invention relates to a method for manufacturing a three-dimensional structure based on a block copolymer. The method comprises the steps of injecting a block copolymer (BCP) solution into each micro-well formed on the substrate and drying it to form a block copolymer layer, and applying a buffer to the block copolymer layer to hydrate the micro-well in three dimensions Forming the structure, after the three-dimensional structure is formed, injecting and curing a hydrogel solution around the three-dimensional structure may include the step of enhancing stability. In particular, the process of hydration by applying a buffer to the micro-well is performed while an electric field is applied. By controlling the concentration of the block copolymer (BCP) and the amplitude and frequency of the electric field, a three-dimensional artificial cell membrane having a desired size and shape, such as a spherical or ciliary shape and high stability (100% survival for 50 days) is manufactured can do. The present invention can be efficiently applied to various biological fields such as artificial cells, cell-mimicking biosensors, and bioreactors.

    SYSTEM FOR DETECTING BIOMOLECULE WI TH HIGH SENSITIVITY USING MICRO-CANT I LEVER
    6.
    发明公开
    SYSTEM FOR DETECTING BIOMOLECULE WI TH HIGH SENSITIVITY USING MICRO-CANT I LEVER 审中-公开
    SYSTEM FORBIOMOLEKÜLNACHWEIS高灵敏度用微型支持

    公开(公告)号：EP2353002A1

    公开(公告)日：2011-08-10

    申请号：EP08878142.2

    申请日：2008-11-11

    Applicant: KIST Korea Institute of Science and Technology

    Inventor： HWANG, Kyo Seon ,  KIM, Tae Song ,  KIM, Sang Kyung ,  LEE, Sang Myung ,  LEE, Yoon Sik

    IPC: G01N33/53

    CPC classification number: G01N33/54373

    Abstract: Provided is a protein detection system using a micro-cantilever and based on immune responses, wherein the micro-cantilever shows significantly improved sensitivity to allow detection of a trace amount of biomolecule. To the micro-cantilever, sandwich immunoassay is applied, and the sandwich immunoassay uses a polyclonal antibody or silica nanoparticles having a monoclonal antibody bound thereto, so that variations in the output signals of the cantilever are amplified and the detection sensitivity is significantly improved. The system enables detection of disease specific antigen at several femtomolar levels, and makes it possible to detect a trace amount of protein related to diseases, particularly to cancers, with ease.

    METHOD FOR DIAGNOSING DISEASES THROUGH OLIGOMER ANALYSIS OF ABNORMALLY AGGREGATED PROTEINS
    10.
    发明公开
    METHOD FOR DIAGNOSING DISEASES THROUGH OLIGOMER ANALYSIS OF ABNORMALLY AGGREGATED PROTEINS 审中-公开
    通过对异常聚集的蛋白质进行低分析来诊断疾病的方法

    公开(公告)号：EP3264093A1

    公开(公告)日：2018-01-03

    申请号：EP16883939.7

    申请日：2016-05-18

    Applicant: Korea Institute of Science and Technology

    Inventor： KANG, Ji Yoon ,  SHIN, Kyeong-Sik ,  JI, Jae-hoon ,  KIM, Tae Song ,  KIM, Youngsoo ,  KIM, Sangyun

    IPC: G01N33/68 ,  G01N33/543 ,  G01N33/535 ,  G01N21/552 ,  G01N27/02

    CPC classification number: G01N33/6896 ,  G01N27/02 ,  G01N27/026 ,  G01N2333/47 ,  G01N2333/4709 ,  G01N2800/28

    Abstract: The present invention relates to a method for diagnosing a disease using an analysis of oligomer of an abnormal aggregated protein includes: (1) preparing a body fluid sample including at least one of blood, blood plasma, blood serum, saliva, urine, tear, and mucus; (2) making a dilution of the body fluid sample; (3) using a biosensor to measure and detect an aggregated protein in the diluted body fluid sample; (4) analyzing a signal change of the biosensor caused by the dilution of the aggregated protein to determine a slope according to the dilution from the measurements; and (5) analyzing a proportion of the oligomer from the slope according to the dilution to make a diagnosis. The method uses a biosensor to measure the impedance and the protein concentration of blood and detects the slope according to the numerical value of the monomer and the oligomer to diagnose normal or abnormal protein aggregation or the associated diseases with more accuracy.

    Abstract translation: 本发明涉及使用异常凝聚蛋白质的低聚物分析来诊断疾病的方法，其包括：（1）制备包括血液，血浆，血清，唾液，尿液，泪液， 和粘液; （2）对体液样品进行稀释; （3）使用生物传感器测量和检测稀释体液样品中的聚集蛋白质; （4）分析由聚集蛋白质的稀释引起的生物传感器的信号变化，以根据测量结果的稀释度确定斜率; 和（5）根据稀释度从斜率分析低聚物的比例以作出诊断。 该方法使用生物传感器测量血液中的阻抗和蛋白质浓度，并根据单体和寡聚体的数值检测斜率，以更准确地诊断正常或异常蛋白质聚集或相关疾病。

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