公开(公告)号：AU2017213725A1

公开(公告)日：2018-08-09

申请号：AU2017213725

申请日：2017-01-30

Applicant: LI COR INC

Inventor： FURTAW MICHAEL D ,  LAMB DONALD T

IPC: H01J49/04 ,  B01D57/02 ,  G01N27/26 ,  G01N27/447 ,  H01J49/16

Abstract: Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.

公开(公告)号：AU2017311109A1

公开(公告)日：2019-02-21

申请号：AU2017311109

申请日：2017-08-07

Applicant: LI COR INC

Inventor： FURTAW MICHAEL D ,  LAMB DONALD T

IPC: G01N27/447

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

公开(公告)号：CA3031226A1

公开(公告)日：2018-02-15

申请号：CA3031226

申请日：2017-08-07

Applicant: LI COR INC

Inventor： FURTAW MICHAEL D ,  LAMB DONALD T ,  MIDDENDORF LYLE R

IPC: G01N27/447 ,  B01L3/00 ,  G01N30/72

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.

公开(公告)号：CA3031212A1

公开(公告)日：2018-02-15

申请号：CA3031212

申请日：2017-08-07

Applicant: LI COR INC

Inventor： FURTAW MICHAEL D ,  LAMB DONALD T

IPC: G01N27/447

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

公开(公告)号：AU2017311109B2

公开(公告)日：2021-01-28

申请号：AU2017311109

申请日：2017-08-07

Applicant: LI COR INC

Inventor： FURTAW MICHAEL D ,  LAMB DONALD T

IPC: G01N27/447

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

公开(公告)号：AU2017311105A1

公开(公告)日：2019-02-21

申请号：AU2017311105

申请日：2017-08-07

Applicant: LI COR INC

Inventor： FURTAW MICHAEL D ,  LAMB DONALD T ,  MIDDENDORF LYLE R

IPC: G01N27/447 ,  B01L3/00 ,  G01N30/72

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.

公开(公告)号：EP2297510A4

公开(公告)日：2014-07-09

申请号：EP09751205

申请日：2009-05-13

Applicant: LI COR INC

Inventor： BOUZID AHMED ,  LAMB DONALD T ,  RAGATZ ANDREW G ,  MIDDENDORF LYLE R

IPC: F21V5/00 ,  G01J3/10 ,  G01J3/44

CPC classification number: G01J3/10 ,  A61B5/0071 ,  G01J3/0208 ,  G01J3/12 ,  G01J3/4406 ,  G01J2003/104 ,  G01J2003/1213 ,  G01J2003/1226 ,  G01N21/6456

公开(公告)号：AU2017213725B2

公开(公告)日：2021-12-23

申请号：AU2017213725

申请日：2017-01-30

Applicant: LI COR INC

Inventor： FURTAW MICHAEL D ,  LAMB DONALD T

IPC: H01J49/04 ,  B01D57/02 ,  G01N27/26 ,  G01N27/447 ,  H01J49/16

Abstract: Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.

公开(公告)号：CA3011620A1

公开(公告)日：2017-08-10

申请号：CA3011620

申请日：2017-01-30

Applicant: LI COR INC

Inventor： FURTAW MICHAEL D ,  LAMB DONALD T

IPC: H01J49/04 ,  B01D57/02 ,  G01N27/26 ,  G01N27/447 ,  H01J49/16

Abstract: Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.

公开(公告)号：CA2724574A1

公开(公告)日：2009-11-26

申请号：CA2724574

申请日：2009-05-13

Applicant: LI COR INC

Inventor： BOUZID AHMED ,  LAMB DONALD T ,  RAGATZ ANDREW G ,  MIDDENDORF LYLE R

IPC: F21V5/00 ,  F21S8/00 ,  G01J1/58

Abstract: An optical system is disclosed that can be used for fluorescence filtering for molecular imaging. In one preferred embodiment, a source subsystem is disclosed comprising a light source and a first set of filters designed to pass wavelengths of light in an absorption band of a fluorescent material. A detector subsystem is also disclosed comprising a light detector, imaging optics, a second set of filters designed to pass wavelengths of light in an emission band of the fluorescent material, and an aperture located at a front focal plane of the imaging optics. A telecentric space is created between the light detector and the imaging optics, such that axial rays from a plurality of field points emerge from the imaging optics parallel to each other and perpendicular to the second set of filters