公开(公告)号：EP2745108A4

公开(公告)日：2015-10-28

申请号：EP12823719

申请日：2012-08-17

Applicant: LIFE TECHNOLOGIES CORP

Inventor： SIKORA MARCIN ,  DAVEY MELVILLE ,  KOLLER CHRISTIAN ,  CAWLEY SIMON ,  WILLIAMS ALAN ,  KULP DAVID

IPC: G06F19/22

CPC classification number: G06F19/22 ,  C12Q1/6869 ,  C12Q2535/122 ,  C12Q2565/607

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.