公开(公告)号：WO2011127429A3

公开(公告)日：2012-02-23

申请号：PCT/US2011031824

申请日：2011-04-08

Applicant: LIFE TECHNOLOGIES CORP ,  SIKORA MARCIN

Inventor： SIKORA MARCIN

IPC: G06F19/10

CPC classification number: G06F19/18 ,  G06F17/30386 ,  G06F19/24

Abstract: Methods and systems for the analysis of genotyping data are presented. According to various embodiments of methods and systems, an angle configuration search may be performed. In various embodiments, an exhaustive search over the entirety of an angle configuration space may be performed to provide a fit to a plurality of angles determined for a plurality of points in a data set generated from a plurality of biological samples. For various embodiments, the angle configuration space may be defined to ensure that a global fit may be determined. According to various methods and systems, a data base of possible angle configurations may be searched, in which each angle configuration may include three angles. According to various methods and systems, a data base of possible angle configurations may include for each angle configuration a probability that the angle configuration may occur.

Abstract translation: 提出了分析基因分型数据的方法和系统。 根据方法和系统的各种实施例，可以执行角度配置搜索。 在各种实施例中，可以执行整个角度配置空间的详尽搜索，以提供对从多个生物样本产生的数据集中的多个点确定的多个角度的拟合。 对于各种实施例，可以定义角度配置空间以确保可以确定全局拟合。 根据各种方法和系统，可以搜索可能的角度配置的数据库，其中每个角度配置可以包括三个角度。 根据各种方法和系统，可能的角度配置的数据库可以包括对于每个角度配置可能发生角度配置的概率。

公开(公告)号：SG11201502797PA

公开(公告)日：2015-05-28

申请号：SG11201502797P

申请日：2013-09-13

Applicant: LIFE TECHNOLOGIES CORP

Inventor： MAJUMDAR NIVEDITA SUMI ,  WESSEL THOMAS ,  WOO DAVID C ,  SIKORA MARCIN ,  JANAWAY GORDON ,  RAIZADA SHWETA ,  LANKESTER JOANNA ,  MARKS JEFFREY ,  WESSEL DANIEL

IPC: G06F19/24

Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

公开(公告)号：SG184483A1

公开(公告)日：2012-11-29

申请号：SG2012074456

申请日：2011-04-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： SIKORA MARCIN

Abstract: Methods and systems for the analysis of genotyping data are presented. According to various embodiments of methods and systems, an angle configuration search may be performed. In various embodiments, an exhaustive search over the entirety of an angle configuration space may be performed to provide a fit to a plurality of angles determined for a plurality of points in a data set generated from a plurality of biological samples. For various embodiments, the angle configuration space may be defined to ensure that a global fit may be determined. According to various methods and systems, a data base of possible angle configurations may be searched, in which each angle configuration may include three angles. According to various methods and systems, a data base of possible angle configurations may include for each angle configuration a probability that the angle configuration may occur.

公开(公告)号：AU2013315167A1

公开(公告)日：2015-04-30

申请号：AU2013315167

申请日：2013-09-13

Applicant: LIFE TECHNOLOGIES CORP

Inventor： MAJUMDAR NIVEDITA SUMI ,  WESSEL THOMAS ,  WOO DAVID C ,  SIKORA MARCIN ,  JANAWAY GORDON ,  RAIZADA SHWETA ,  LANKESTER JOANNA ,  MARKS JEFFREY ,  WESSEL DANIEL

IPC: G06F19/24

Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

公开(公告)号：EP2556459A4

公开(公告)日：2016-11-02

申请号：EP11766837

申请日：2011-04-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： SIKORA MARCIN

IPC: G06F19/18 ,  G06F19/24

CPC classification number: G06F19/18 ,  G06F17/30386 ,  G06F19/24

Abstract: Methods and systems for the analysis of genotyping data are presented. According to various embodiments of methods and systems, an angle configuration search may be performed. In various embodiments, an exhaustive search over the entirety of an angle configuration space may be performed to provide a fit to a plurality of angles determined for a plurality of points in a data set generated from a plurality of biological samples. For various embodiments, the angle configuration space may be defined to ensure that a global fit may be determined. According to various methods and systems, a data base of possible angle configurations may be searched, in which each angle configuration may include three angles. According to various methods and systems, a data base of possible angle configurations may include for each angle configuration a probability that the angle configuration may occur.

公开(公告)号：EP2745108A4

公开(公告)日：2015-10-28

申请号：EP12823719

申请日：2012-08-17

Applicant: LIFE TECHNOLOGIES CORP

Inventor： SIKORA MARCIN ,  DAVEY MELVILLE ,  KOLLER CHRISTIAN ,  CAWLEY SIMON ,  WILLIAMS ALAN ,  KULP DAVID

IPC: G06F19/22

CPC classification number: G06F19/22 ,  C12Q1/6869 ,  C12Q2535/122 ,  C12Q2565/607

Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.