公开(公告)号：AU2020225632A1

公开(公告)日：2021-09-02

申请号：AU2020225632

申请日：2020-02-21

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YU XIN ,  DE MOLLERAT DU JEU XAVIER ,  LIU CHAO YAN ,  LIU JIAN ,  ZMUDA JONATHAN

IPC: C07K14/005 ,  C12N15/86

Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2x10

公开(公告)号：CA3130108A1

公开(公告)日：2020-08-27

申请号：CA3130108

申请日：2020-02-21

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YU XIN ,  DE MOLLERAT DU JEU XAVIER ,  LIU CHAO YAN ,  LIU JIAN ,  ZMUDA JONATHAN

IPC: C07K14/005 ,  C12N15/86

Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2x1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.