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公开(公告)号：KR20180030613A
公开(公告)日：2018-03-23
申请号：KR20187003996
申请日：2016-07-13
Applicant: LIFE TECHNOLOGIES CORP
Inventor： ZMUDA JONATHAN , LIU CHAO YAN
IPC: C12P21/02 , C07K14/505 , C07K16/00 , C12N5/00
CPC classification number: C12P21/02 , C07K14/505 , C07K16/00 , C07K2317/14 , C07K2317/21 , C07K2317/92 , C12N5/0043 , C12N2510/02 , C12N2511/00
Abstract: 본발명은일반적으로현탁 CHO 세포중의재조합단백질의높은수준의발현에적합한시스템및 방법에관한것이다. 특히, 본발명은본 발명의도입이상기과정동안에성장배지를대체하거나재충전하거나보출할필요를제거하게끔한다. 본발명은또한현탁 CHO 세포를형질전환/형질감염시키는데유용한조성물및 키트에관한것이다.
Abstract translation: 本发明一般涉及适用于在悬浮的CHO细胞中高水平表达重组蛋白的系统和方法。 具体而言，本发明消除了在本发明的引入阶段期间替换，再充注或分配生长培养基的需要。 本发明还涉及用于转化/转染悬浮CHO细胞的组合物和试剂盒。
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公开(公告)号：CA3051089A1
公开(公告)日：2013-11-07
申请号：CA3051089
申请日：2013-05-02
Applicant: LIFE TECHNOLOGIES CORP
Inventor： VASU SANJAY K , CHIOU HENRY C , ROGERS JEFFREY , CISNEROS MARIA , LI JINGQIU , LIU CHAO YAN , JONES MEREDITH
Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.
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公开(公告)号：CA2872476C
公开(公告)日：2020-07-07
申请号：CA2872476
申请日：2013-05-02
Applicant: LIFE TECHNOLOGIES CORP
Inventor： VASU SANJAY K , CHIOU HENRY C , ROGERS JEFFREY , CISNEROS MARIA , LI JINGQIU , LIU CHAO YAN , JONES MEREDITH
Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.
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Patent Agency Ranking
公开(公告)号：CA3130108A1
公开(公告)日：2020-08-27
申请号：CA3130108
申请日：2020-02-21
Applicant: LIFE TECHNOLOGIES CORP
Inventor： YU XIN , DE MOLLERAT DU JEU XAVIER , LIU CHAO YAN , LIU JIAN , ZMUDA JONATHAN
IPC: C07K14/005 , C12N15/86
Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2x1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.
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公开(公告)号：CA2992096A1
公开(公告)日：2017-01-19
申请号：CA2992096
申请日：2016-07-13
Applicant: LIFE TECHNOLOGIES CORP
Inventor： ZMUDA JONATHAN , LIU CHAO YAN
Abstract: The present invention is directed generally to systems and methods suitable for high level expression of recombinant proteins in suspension CHO cells. In particular, the invention allows introduction of the invention obviates the need to replace, replenish or supplement the growth medium during the procedure. The invention also relates to compositions and kits useful for culturing and transforming/transfecting suspension CHO cells.
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公开(公告)号：AU2020225632A1
公开(公告)日：2021-09-02
申请号：AU2020225632
申请日：2020-02-21
Applicant: LIFE TECHNOLOGIES CORP
Inventor： YU XIN , DE MOLLERAT DU JEU XAVIER , LIU CHAO YAN , LIU JIAN , ZMUDA JONATHAN
IPC: C07K14/005 , C12N15/86
Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2x10
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公开(公告)号：CA2872476A1
公开(公告)日：2013-11-07
申请号：CA2872476
申请日：2013-05-02
Applicant: LIFE TECHNOLOGIES CORP
Inventor： VASU SANJAY K , CHIOU HENRY C , ROGERS JEFFREY , CISNEROS MARIA , LI JINGQIU , LIU CHAO YAN , JONES MEREDITH
IPC: C12N5/00
Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.
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公开(公告)号：CA3051089C
公开(公告)日：2022-04-12
申请号：CA3051089
申请日：2013-05-02
Applicant: LIFE TECHNOLOGIES CORP
Inventor： VASU SANJAY K , CHIOU HENRY C , ROGERS JEFFREY , CISNEROS MARIA , LI JINGQIU , LIU CHAO YAN , JONES MEREDITH
Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.
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