公开(公告)号：AU2013249123A1

公开(公告)日：2014-12-11

申请号：AU2013249123

申请日：2013-04-19

Applicant: LIFE TECHNOLOGIES CORP

Inventor： CHEN CAIFU ,  MCFARLAND CASEY R ,  KEYS JR DAVID N

IPC: C12Q1/68

Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

公开(公告)号：IN9784DEN2014A

公开(公告)日：2015-07-31

申请号：IN9784DEN2014

申请日：2014-11-19

Applicant: LIFE TECHNOLOGIES CORP

Inventor： CHEN CAIFU ,  MCFARLAND CASEY R ,  KEYS JR DAVID N

IPC: C12Q1/68

Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present , counting the number of fractions with an amplified target nucleic acid , and determining the quantity of target nucleic acid in the sample.