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    METHODS, COMPOSITIONS, AND KITS FOR DETECTING ALLELIC VARIANTS
    2.
    发明申请
    METHODS, COMPOSITIONS, AND KITS FOR DETECTING ALLELIC VARIANTS 审中-公开
    用于检测ALLELIC变体的方法，组合物和试剂盒

    公开(公告)号：WO2010111682A3

    公开(公告)日：2011-03-10

    申请号：PCT/US2010028963

    申请日：2010-03-26

    Applicant: LIFE TECHNOLOGIES CORP ,  CHEN CAIFU ,  TAN RUOYING

    Inventor： CHEN CAIFU ,  TAN RUOYING

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

    Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR ("cast-PCR").

    Abstract translation: 在一些实施方案中，本发明一般涉及用于区分不同等位基因之间的序列变异的组合物，方法和试剂盒。 更具体地说，在一些实施方案中，本发明提供了用于定量稀少（例如，突变体）等位基因变体如SNP或核苷酸（NT）插入或缺失的组合物，方法和试剂盒，其包含丰富的（例如，野生型 ）等位基因变体，具有高特异性和选择性。 特别地，在一些实施方案中，本发明涉及用于突变检测的高选择性方法，称为竞争性等位基因特异性TaqMan PCR（“cast-PCR”）。

    Method of performing digital PCR
    7.
    发明专利
    Method of performing digital PCR 未知

    公开(公告)号：AU2013249123A1

    公开(公告)日：2014-12-11

    申请号：AU2013249123

    申请日：2013-04-19

    Applicant: LIFE TECHNOLOGIES CORP

    Inventor： CHEN CAIFU ,  MCFARLAND CASEY R ,  KEYS JR DAVID N

    IPC: C12Q1/68

    Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

    METHODS, COMPOSITIONS, AND KITS FOR DETECTING RARE CELLS
    8.
    发明申请
    METHODS, COMPOSITIONS, AND KITS FOR DETECTING RARE CELLS 审中-公开
    用于检测稀有细胞的方法，组合物和试剂盒

    公开(公告)号：WO2012097353A1

    公开(公告)日：2012-07-19

    申请号：PCT/US2012021397

    申请日：2012-01-14

    Applicant: LIFE TECHNOLOGIES CORP ,  DENG DAVID XINGFEI ,  CHEN CAIFU

    Inventor： DENG DAVID XINGFEI ,  CHEN CAIFU

    IPC: C12Q1/68 ,  G01N33/50

    CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/156

    Abstract: Disclosed herein are methods for identifying rare cells containing particular markers and/or alleles from biological samples that have not been substantially pre-processed (e.g., unprocessed whole blood). The methods described herein provide a system for digital enrichment of target cells from a biological sample and detection of such target cells, thereby allowing accurate and efficient detection and / or enumeration of such cells in the sample.

    Abstract translation: 本文公开了用于从未经过基本上预处理的生物样品（例如未加工的全血）鉴定含有特定标记物和/或等位基因的稀有细胞的方法。 本文描述的方法提供了用于从生物样品数字富集靶细胞并检测这样的靶细胞的系统，从而允许样品中这些细胞的准确和有效的检测和/或计数。

    METHYLATION-SPECIFIC COMPETITIVE ALLELE-SPECIFIC TAQMAN POLYMERASE CHAIN REACTION (CAST-PCR)
    9.
    发明申请
    METHYLATION-SPECIFIC COMPETITIVE ALLELE-SPECIFIC TAQMAN POLYMERASE CHAIN REACTION (CAST-PCR) 审中-公开
    甲基化特异性竞争性特异性TAQMAN聚合酶链反应（CAST-PCR）

    公开(公告)号：WO2011139920A2

    公开(公告)日：2011-11-10

    申请号：PCT/US2011034675

    申请日：2011-04-29

    Applicant: LIFE TECHNOLOGIES CORP ,  CHEN CAIFU

    Inventor： CHEN CAIFU

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/6851 ,  C12Q2523/125 ,  C12Q2561/113

    Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating between different methylated and/or unmethylated nucleic acid loci. In certain embodiments, the inventions provides for detecting or quantitating undifferentiated embryonic stem cells in a population of differentiated cells. The invention is also useful for discriminating between fetal versus maternal cells, or healthy versus infected cells, or normal versus cancerous cells, or detecting reduction in viral load or measuring therapeutic efficiency in a patient, and more.

    Abstract translation: 在一些实施方案中，本发明一般涉及用于区分不同甲基化和/或未甲基化核酸基因座的组合物，方法和试剂盒。 在某些实施方案中，本发明提供了在分化细胞群中检测或定量未分化的胚胎干细胞。 本发明还可用于区分胎儿与母体细胞或健康对感染细胞或正常与癌细胞，或检测患者病毒载量的降低或测量患者的治疗效果等。

    METHODS, COMPOSITIONS, AND KITS FOR DETECTING ALLELIC VARIANTS
    10.
    发明申请
    METHODS, COMPOSITIONS, AND KITS FOR DETECTING ALLELIC VARIANTS 审中-公开
    用于检测等位变体的方法，组合物和试剂盒

    公开(公告)号：WO2010077324A2

    公开(公告)日：2010-07-08

    申请号：PCT/US2009006652

    申请日：2009-12-17

    Applicant: LIFE TECHNOLOGIES CORP ,  CHEN CAIFU ,  TAN RUOYING

    Inventor： CHEN CAIFU ,  TAN RUOYING

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/6886 ,  C07H21/04 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161

    Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR ("cast-PCR").

    Abstract translation: 在一些实施方案中，本发明一般涉及用于区分不同等位基因之间的序列变异的组合物，方法和试剂盒。 更具体而言，在一些实施方案中，本发明提供了用于定量包含丰富（例如野生型）的样品中的罕见（例如，突变）等位基因变体例如SNP或核苷酸（NT）插入或缺失的组合物，方法和试剂盒 ）具有高特异性和选择性的等位基因变体。 特别地，在一些实施方案中，本发明涉及用于突变检测的高度选择性方法，称为竞争性等位基因特异性TaqMan PCR（“cast-PCR”）。

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