公开(公告)号：WO2014066404A3

公开(公告)日：2014-08-07

申请号：PCT/US2013066204

申请日：2013-10-22

Applicant: LIFE TECHNOLOGIES CORP

Inventor： ROGERS JEFFREY ,  FLETCHER JULIA ,  LOWITZ KEVIN

IPC: C12N15/10 ,  C12N15/62

CPC classification number: C12N15/67 ,  C07K2/00 ,  C07K2319/40 ,  C07K2319/60 ,  C12N9/2471 ,  C12N15/1086 ,  C12N15/63 ,  C12N15/70 ,  C12P21/00

Abstract: The present disclosure describes a novel recombinant gene expression system and a method of its use. The recombinant gene expression system is adapted to enable a user to monitor the successful expression of a protein in real time by allowing a small portion (e.g., less than 10%) of the target protein to be expressed as a fusion with a readily detectable reporter. The recombinant gene expression system further allows a user to select a clone that exhibits high, medium or low expression by providing a random ribosomal binding site having the nucleotide sequence N4R6NX, where N is A, T, G, or C, where R is A or G, and where x is an integer from 6 to 11 upstream of the cloned gene. By selecting a clone exhibiting a desired relative level of reporter gene expression, a user can tailor the expression level of the desired protein.

Abstract translation: 本公开描述了新的重组基因表达系统及其使用方法。 重组基因表达系统适合于使用户能够通过允许一小部分（例如，少于10％）的靶蛋白被表达为与易于检测的报道分子的融合物来实时监测蛋白质的成功表达 。 通过提供具有核苷酸序列N4R6NX的随机核糖体结合位点，其中N是A，T，G或C，其中R是A，所述重组基因表达系统还允许用户选择表现高，中或低表达的克隆 或G，并且其中x是克隆基因上游6至11的整数。 通过选择展现出期望的报道基因表达相对水平的克隆，用户可以调整所需蛋白质的表达水平。

公开(公告)号：WO2015112767A3

公开(公告)日：2015-10-22

申请号：PCT/US2015012534

申请日：2015-01-22

Applicant: LIFE TECHNOLOGIES CORP

Inventor： ROGERS JEFFREY ,  POTTER JASON

IPC: C12N9/22 ,  C12N9/12

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12P19/34 ,  C12Y207/07049

Abstract: The invention provides novel reverse transcriptases (RTs) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.

Abstract translation: 本发明提供了具有期望性质例如增加的热稳定性，增加的热反应性和/或增加的对抑制剂的抗性的新型逆转录酶（RT）。 在某些实施方案中，本发明提供了使用包含这种新型逆转录酶的试剂盒，组合物和/或反应混合物产生，扩增和/或测序核酸分子（特别是cDNA分子）的方法。

公开(公告)号：CA2872476A1

公开(公告)日：2013-11-07

申请号：CA2872476

申请日：2013-05-02

Applicant: LIFE TECHNOLOGIES CORP

Inventor： VASU SANJAY K ,  CHIOU HENRY C ,  ROGERS JEFFREY ,  CISNEROS MARIA ,  LI JINGQIU ,  LIU CHAO YAN ,  JONES MEREDITH

IPC: C12N5/00

Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

公开(公告)号：CA3051089A1

公开(公告)日：2013-11-07

申请号：CA3051089

申请日：2013-05-02

Applicant: LIFE TECHNOLOGIES CORP

Inventor： VASU SANJAY K ,  CHIOU HENRY C ,  ROGERS JEFFREY ,  CISNEROS MARIA ,  LI JINGQIU ,  LIU CHAO YAN ,  JONES MEREDITH

IPC: C12P21/02 ,  C12N5/02 ,  C12N5/071 ,  C12N5/10 ,  C12N15/67 ,  C12N15/87 ,  C12P1/00

Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

公开(公告)号：CA3051089C

公开(公告)日：2022-04-12

申请号：CA3051089

申请日：2013-05-02

Applicant: LIFE TECHNOLOGIES CORP

Inventor： VASU SANJAY K ,  CHIOU HENRY C ,  ROGERS JEFFREY ,  CISNEROS MARIA ,  LI JINGQIU ,  LIU CHAO YAN ,  JONES MEREDITH

IPC: C12P21/02 ,  C12N5/02 ,  C12N5/071 ,  C12N5/10 ,  C12N15/67 ,  C12N15/87 ,  C12P1/00

Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

公开(公告)号：CA2872476C

公开(公告)日：2020-07-07

申请号：CA2872476

申请日：2013-05-02

Applicant: LIFE TECHNOLOGIES CORP

Inventor： VASU SANJAY K ,  CHIOU HENRY C ,  ROGERS JEFFREY ,  CISNEROS MARIA ,  LI JINGQIU ,  LIU CHAO YAN ,  JONES MEREDITH

IPC: C12N5/00 ,  C12N5/071 ,  C12N5/10 ,  C12N15/67 ,  C12N15/85 ,  C12N15/87 ,  C12P1/00 ,  C12P21/02

Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.