公开(公告)号：WO2016115001A3

公开(公告)日：2016-09-09

申请号：PCT/US2016012785

申请日：2016-01-11

Applicant: LIFE TECHNOLOGIES CORP

Inventor： BEROSIK STEPHAN ,  GAO JIANBO ,  CHEN SHIAW-MIN ,  WENZ H MICHAEL

IPC: C12N15/09

CPC classification number: C12N15/1093 ,  C12Q2535/122 ,  C12Q2537/113 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/173

Abstract: Methods, systems, kits and compositions are described for quality control and quantitation of nucleic acid libraries of double stranded nucleic acid libraries prior to massively parallel sequencing. Electrophoretic separation within a channel using a detectably labeled single stranded sizing ladder may be used to define the molecular weight range and amount of the double stranded nucleic acids.

Abstract translation: 描述了在大规模平行测序之前对双链核酸文库的核酸文库进行质量控制和定量的方法，系统，试剂盒和组合物。 使用可检测标记的单链上浆梯在通道内进行电泳分离可用于确定双链核酸的分子量范围和量。