公开(公告)号：WO2012142003A3

公开(公告)日：2013-02-28

申请号：PCT/US2012032802

申请日：2012-04-09

Applicant: LIFE TECHNOLOGIES CORP ,  YING LAI-QIANG ,  BRANCHAUD BRUCE

Inventor： YING LAI-QIANG ,  BRANCHAUD BRUCE

IPC: C12Q1/68 ,  G01N33/48 ,  G01N33/542

CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  G01N33/542 ,  C12Q2523/109 ,  C12Q2525/155

Abstract: Methods comprising chemical ligation of oligonucleotides are provided. In some embodiments, methods of detecting a polymorphisms in nucleic acids are provided. In some embodiments, methods of detecting at least one analyte are provided. In some embodiments, methods of labeling solid support particles are provided. Kits comprising oligonucleotides with chemically ligatable moieties are also provided.

Abstract translation: 提供包括化学连接寡核苷酸的方法。 在一些实施方案中，提供了检测核酸多态性的方法。 在一些实施方案中，提供了检测至少一种分析物的方法。 在一些实施方案中，提供了标记固体支持物颗粒的方法。 还提供了包含具有化学可连接部分的寡核苷酸的试剂盒。

公开(公告)号：EP2635350A4

公开(公告)日：2016-07-27

申请号：EP11838687

申请日：2011-11-01

Applicant: LIFE TECHNOLOGIES CORP

Inventor： KANG HEE CHOL ,  GEE KYLE ,  MANDAVILLI BHASKAR ,  YING LAI-QIANG ,  BRANCHAUD BRUCE

IPC: A61Q5/10 ,  A61K8/41 ,  C09B23/08

CPC classification number: G01N21/6486 ,  C09B23/06 ,  C09B23/08 ,  C09B23/083 ,  C09B57/00

Abstract: The present invention is directed to compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced dyes, including Cy-based hydrocyanine dyes and Cy-based deuterocyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

公开(公告)号：EP2633062A4

公开(公告)日：2014-08-13

申请号：EP11837225

申请日：2011-10-28

Applicant: LIFE TECHNOLOGIES CORP

Inventor： YING LAI-QIANG ,  YUE STEPHEN ,  BRANCHAUD BRUCE ,  ZHANG YU-ZHONG

IPC: G01N33/532 ,  C07D495/04 ,  C08B37/00 ,  G01N33/543 ,  G01N33/58

CPC classification number: C07D495/04 ,  C07B2200/11 ,  C07D403/12 ,  C07K1/13 ,  C07K1/22 ,  C07K16/42 ,  C07K2317/20 ,  C07K2317/92 ,  C08B37/00 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1088 ,  C12N5/0636 ,  C12N5/0637 ,  C12N5/0638 ,  G01N33/532 ,  G01N33/533 ,  G01N33/54353 ,  G01N33/58 ,  G01N33/582 ,  G01N33/6803

Abstract: Biotin derivatives, methods of using the biotin derivatives and kits comprising the biotin derivatives.

Abstract translation: 生物素衍生物，使用生物素衍生物的方法和包含生物素衍生物的试剂盒。