中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

9 records (took 0.041 seconds)

    SYSTEM AND METHOD FOR AUTOMATED REPEAT SEQUENCING
    1.
    发明申请
    SYSTEM AND METHOD FOR AUTOMATED REPEAT SEQUENCING 审中-公开

    公开(公告)号：WO2022104138A1

    公开(公告)日：2022-05-19

    申请号：PCT/US2021/059242

    申请日：2021-11-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： WONG, Chiu Tai Andrew ,  SZETO, Kylan ,  SHANKAR, Shanti ,  BEAUCHEMIN, Mark

    IPC: C12Q1/6869

    Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

    METHOD AND APPARATUS FOR CALIBRATION OF A SENSOR ARRAY
    2.
    发明申请
    METHOD AND APPARATUS FOR CALIBRATION OF A SENSOR ARRAY 审中-公开
    传感器阵列校准的方法和装置

    公开(公告)号：WO2013096897A1

    公开(公告)日：2013-06-27

    申请号：PCT/US2012/071471

    申请日：2012-12-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： REARICK, Todd ,  DAVEY, Melville ,  BEAUCHEMIN, Mark

    IPC: G01N27/414

    CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

    Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. Preferably, the sensor array consists of a plurality of ISFETs which are in fluid communication with a reference electrode. A particular reference voltage is determined, which depends on the maximum value of the overall average gain of the sensors in an array, but also on a maximum number of sensors available for the measurement. The method includes sweeping a voltage of the reference electrode from a first voltage to a second voltage. The output voltage of each of the plurality of sensors is monitored at one or more voltages within the first and second voltages. The gain of each sensor at each of the one or more voltages is determined. An overall average gain of the plurality of sensors is calculated at each of the one or more voltages. Further, an acquisition window for the sensor array is determined. The acquisition window includes a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

    Abstract translation: 提供了一种方法，计算机程序产品和系统来校准具有多个传感器的传感器阵列。 优选地，传感器阵列由与参考电极流体连通的多个ISFET组成。 确定特定参考电压，其取决于阵列中传感器的总体平均增益的最大值，也取决于可用于测量的最大传感器数量。 该方法包括将参考电极的电压从第一电压扫到第二电压。 在第一和第二电压内的一个或多个电压下监视多个传感器中的每一个的输出电压。 确定在一个或多个电压中的每一个处的每个传感器的增益。 在一个或多个电压中的每一个处计算多个传感器的总平均增益。 此外，确定传感器阵列的采集窗口。 采集窗口包括在特定参考电极电压下提供最大总平均增益的传感器的最大分布。

    DATA COMPRESSION OF WAVEFORMS ASSOCIATED WITH A CHEMICAL EVENT OCCURING ON A SENSOR ARRAY
    4.
    发明申请
    DATA COMPRESSION OF WAVEFORMS ASSOCIATED WITH A CHEMICAL EVENT OCCURING ON A SENSOR ARRAY 审中-公开
    与传感器阵列相关的化学事件相关的波形的数据压缩

    公开(公告)号：WO2013096906A1

    公开(公告)日：2013-06-27

    申请号：PCT/US2012/071482

    申请日：2012-12-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： REARICK, Todd ,  BEAUCHEMIN, Mark

    IPC: G01N27/414 ,  G06F19/22 ,  H03M7/30

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/27 ,  G01N27/4148 ,  G06F19/22

    Abstract: A method, a computer program and an apparatus for measuring a waveform associated with a chemical event occurring on an ISFET sensor array. The waveform includes at least one region associated with expected measured values and at least one region associated with unpredictable measured values. First frame averaging is applied to at least one region associated with the expected measured values. Second frame averaging is applied to at least one region associated with the unpredictable measured values. The second number of frames used for averaging is less than the first number of frames. By the variable frame averaging the amount of stored data can be reduced.

    Abstract translation: 一种用于测量与在ISFET传感器阵列上发生的化学事件相关联的波形的方法，计算机程序和装置。 波形包括与预期测量值相关联的至少一个区域和与不可预测的测量值相关联的至少一个区域。 将第一帧平均应用于与预期测量值相关联的至少一个区域。 将第二帧平均应用于与不可预测的测量值相关联的至少一个区域。 用于平均的第二帧数小于第一帧数。 通过可变帧平均化，可以减少存储的数据量。

    AUTOMATED ON-INSTRUMENT pH ADJUSTMENT
    5.
    发明申请
    AUTOMATED ON-INSTRUMENT pH ADJUSTMENT 审中-公开
    自动化仪器pH调节

    公开(公告)号：WO2013012463A1

    公开(公告)日：2013-01-24

    申请号：PCT/US2012/032571

    申请日：2012-04-06

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  MARRAN, David ,  BEAUCHEMIN, Mark

    Inventor： MARRAN, David ,  BEAUCHEMIN, Mark

    IPC: G01N27/414

    CPC classification number: G01N33/84 ,  C12Q1/6874

    Abstract: A method of preparing a sequencing device includes determining a sensitivity of a pH of a solution to a first reagent, determining an amount of the first reagent to add to the solution to approach a target pH, adding the amount of the first reagent to the solution, and diluting a nucleotide solution with the solution.

    Abstract translation: 制备测序装置的方法包括确定溶液的pH对第一试剂的灵敏度，确定添加到溶液中的第一试剂的量以接近目标pH，将第一试剂的量加到溶液中 ，并用溶液稀释核苷酸溶液。

    METHOD AND APPARATUS FOR CALIBRATION OF A SENSOR ARRAY
    8.
    发明授权
    METHOD AND APPARATUS FOR CALIBRATION OF A SENSOR ARRAY 有权
    方法和设备校准传感器装置

    公开(公告)号：EP2800968B1

    公开(公告)日：2017-03-22

    申请号：EP12818971.9

    申请日：2012-12-21

    Applicant: Life Technologies Corporation

    Inventor： REARICK, Todd ,  DAVEY, Melville ,  BEAUCHEMIN, Mark

    IPC: G01N27/414

    CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

    Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

    METHOD AND APPARATUS FOR CALIBRATION OF A SENSOR ARRAY
    9.
    发明公开
    METHOD AND APPARATUS FOR CALIBRATION OF A SENSOR ARRAY 有权
    VERFAHREN UND VORRICHTUNG ZUR KALIBRIERUNG EINER SENSORANORDNUNG

    公开(公告)号：EP2800968A1

    公开(公告)日：2014-11-12

    申请号：EP12818971.9

    申请日：2012-12-21

    Applicant: Life Technologies Corporation

    Inventor： REARICK, Todd ,  DAVEY, Melville ,  BEAUCHEMIN, Mark

    IPC: G01N27/414

    CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

    Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

    Abstract translation: 提供了一种方法，计算机程序产品和系统来校准具有多个传感器的传感器阵列。 该方法可以包括将参考电极的电压从第一电压扫描到第二电压，其中参考电极与传感器阵列流体连通。 可以在第一和第二电压内的一个或多个电压下监视多个传感器中的每一个的输出电压。 可以在一个或多个电压中的每一个处计算多个传感器的总体平均增益。 此外，可以确定传感器阵列的采集窗口。 采集窗口可以包括在特定参考电极电压下提供最大总平均增益的传感器的最大分布。

Patent Agency Ranking