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    METHODS AND SYSTEMS FOR CULTURING MICROBIAL AND CELLULAR SEED CULTURES
    1.
    发明申请
    METHODS AND SYSTEMS FOR CULTURING MICROBIAL AND CELLULAR SEED CULTURES 审中-公开
    培养微生物和细胞种子文化的方法和系统

    公开(公告)号：WO2016057216A1

    公开(公告)日：2016-04-14

    申请号：PCT/US2015/051590

    申请日：2015-09-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： BROWN, Jason D.

    IPC: C12M1/00 ,  C12M3/00 ,  C12M3/06

    CPC classification number: A01G22/00 ,  A01G9/02 ,  C12M23/14 ,  C12M23/28 ,  C12M23/46 ,  C12M27/16 ,  C12M27/20

    Abstract: A method for culturing a microbial or cellular seed culture includes positioning a first flexible bag (68) within a chamber (40) of a first retainer (14), the first retainer (14) being secured to a mixer table (12). A culture (82) is produced within a compartment (80) of the flexible bag (68), the culture (82) comprising a growth media and a microbial or cellular starter culture added thereto. The mixer table (12) is activated so that the culture (82) is mixed within the compartment (80) of the first flexible bag (68) while the microbes or cells of the culture grow, the culture (82) having a maximum volume of less than 10 liters.

    Abstract translation: 用于培养微生物或细胞种子培养的方法包括将第一柔性袋（68）定位在第一保持器（14）的室（40）内，第一保持器（14）固定到混合器台（12）。 在柔性袋（68）的隔室（80）内产生培养物（82），所述培养物（82）包含生长培养基和添加到其中的微生物或细胞发酵剂培养物。 搅拌台（12）被激活，使得培养物（82）在培养物的微生物或细胞生长的同时在第一柔性袋（68）的隔室（80）内混合，培养物（82）具有最大体积 小于10升。

    FLUID MIXING SYSTEMS WITH MODULAR IMPELLERS AND RELATED METHODS
    2.
    发明申请
    FLUID MIXING SYSTEMS WITH MODULAR IMPELLERS AND RELATED METHODS 审中-公开

    公开(公告)号：WO2021158555A1

    公开(公告)日：2021-08-12

    申请号：PCT/US2021/016224

    申请日：2021-02-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： JONES, Nephi D. ,  BROWN, Jason D.

    IPC: B01F7/00 ,  B01F15/00 ,  B01F15/06

    Abstract: A mixing system for mixing a liquid includes a first impeller segment (170A) having a first mount (172A) and a first mixing blade (174A) secured to the first mount (172A) and a second impeller segment (170B) having a second mount (172B) and a first mixing blade (174B) secured to the second mount (172B), the second impeller segment (170B) being separate and discrete from the first impeller segment (170A). One or more drive members are secured to the first impeller segment (170A) and the second impeller segment (170B) for concurrently rotating the first impeller segment (170A) and the second impeller segment (170B) about a rotational axis (171). The first impeller segment (170A) and the second impeller segment (170B) are secured to the one or more drive members so that a plane (210) extending normal to the axis (171) of rotation intersects with the first mixing blade (174A) of the first impeller segment (170A) and the first mixing blade (174A) of the second impeller segment (170B).

    GAS FILTER SYSTEMS FOR FLUID PROCESSING SYSTEMS
    4.
    发明公开
    GAS FILTER SYSTEMS FOR FLUID PROCESSING SYSTEMS 审中-公开

    公开(公告)号：EP4269554A2

    公开(公告)日：2023-11-01

    申请号：EP23192479.6

    申请日：2014-12-31

    Applicant: Life Technologies Corporation

    Inventor： BROWN, Jason D. ,  STAHELI, Clinton C. ,  JONES, Nephi D.

    IPC: C12M1/12

    Abstract: A filter assembly includes a casing having of a polymeric film and bounding a compartment, the casing having an inlet opening communicating with the compartment and a first outlet opening communicating with the compartment. A first filter includes a porous filter body through which gas can pass and has a pore size smaller than 1 µm, the first filter being coupled with the casing so that the filter body is at least partially disposed within compartment of the casing and so that gas passing through the compartment of the casing from the inlet opening to the first outlet opening must pass through the filter body.

    CONDENSER SYSTEMS FOR FLUID PROCESSING SYSTEMS
    5.
    发明公开
    CONDENSER SYSTEMS FOR FLUID PROCESSING SYSTEMS 审中-公开

    公开(公告)号：EP4324906A1

    公开(公告)日：2024-02-21

    申请号：EP23205290.2

    申请日：2014-12-31

    Applicant: Life Technologies Corporation

    Inventor： BROWN, Jason D. ,  STAHELI, Clinton C ,  MEEKS, Anthony D.

    IPC: C12M1/00

    Abstract: A condenser bag includes a body bounding a channel that extends between an inlet opening at a first end and an outlet opening and an opposing second end, the body being comprised of a polymeric film. An intake port is secured to the body at the inlet opening and bounds an port opening that extends therethrough and communicates with the channel. A tubular transfer line has a first end coupled with the body so as to communicate with the channel at a first location located between the inlet opening and the outlet opening and an opposing second end coupled with either the intake port so as to communicate with the port opening therein or with the body so as to communicate with the channel at a second location located between the intake port and the first location.

    METHODS AND SYSTEMS FOR CULTURING MICROBIAL AND CELLULAR SEED CULTURES
    7.
    发明公开
    METHODS AND SYSTEMS FOR CULTURING MICROBIAL AND CELLULAR SEED CULTURES 审中-公开
    用于培养微生物和细胞种子培养物的方法和系统

    公开(公告)号：EP3204484A1

    公开(公告)日：2017-08-16

    申请号：EP15771495.7

    申请日：2015-09-23

    Applicant: Life Technologies Corporation

    Inventor： BROWN, Jason D.

    IPC: C12M1/00 ,  C12M3/00 ,  C12M3/06

    CPC classification number: A01G22/00 ,  A01G9/02 ,  C12M23/14 ,  C12M23/28 ,  C12M23/46 ,  C12M27/16 ,  C12M27/20

    Abstract: A method for culturing a microbial or cellular seed culture includes positioning a first flexible bag within a chamber of a first retainer, the first retainer being secured to a mixer table. A culture is produced within a compartment of the flexible bag, the culture comprising a growth media and a microbial or cellular starter culture added thereto. The mixer table is activated so that the culture is mixed within the compartment of the first flexible bag while the microbes or cells of the culture grow, the culture having a maximum volume of less than 10 liters.

    Abstract translation: 用于培养微生物或细胞种子培养物的方法包括将第一柔性袋放置在第一保持器的腔室内，第一保持器被固定到混合台上。 在柔性袋的隔室内产生培养物，所述培养物包含生长培养基和添加到其中的微生物或细胞发酵剂培养物。 激活混合台，使得培养物在第一柔性袋的隔室内混合，同时培养物的微生物或细胞生长，培养物的最大体积小于10升。

    CONDENSER SYSTEMS FOR FLUID PROCESSING SYSTEMS
    10.
    发明公开
    CONDENSER SYSTEMS FOR FLUID PROCESSING SYSTEMS 审中-公开

    公开(公告)号：EP3466509A1

    公开(公告)日：2019-04-10

    申请号：EP18208107.5

    申请日：2014-12-31

    Applicant: Life Technologies Corporation

    Inventor： BROWN, Jason D. ,  STAHELI, Clinton C. ,  MEEKS, Anthony D.

    IPC: B01D5/00 ,  B65D88/16 ,  B01F15/00 ,  B01F3/04

    Abstract: A system for mixing a liquid solution or suspension, comprising a container assembly and a condenser bag (102). The container assembly comprises a collapsible bag (12) bounding a compartment, the collapsible bag being comprised of polymeric film and adapted to hold a fluid; a sparger at a lower end of the collapsible bag for delivering gas into the compartment; an exhaust port secured to an upper end of the collapsible bag and bounding a port opening that communicates with the compartment. The condenser bag comprises: a body (164) bounding a channel (178) that extends between an inlet opening (184) at a first end (179) and an outlet opening (185) at an opposing second end (181), the body being comprised of a polymeric film; and an intake port (166) secured to the body at the inlet opening and bounding a port opening that communicates with the channel. The intake port of the condenser bag is coupled with the exhaust port on the collapsible bag so that at least part of the body is disposed at an elevation above the upper end of the collapsible bag.

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