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    SYSTEMS AND METHOD FOR FLUORESCENCE IMAGING
    1.
    发明申请
    SYSTEMS AND METHOD FOR FLUORESCENCE IMAGING 审中-公开
    用于荧光成像的系统和方法

    公开(公告)号：WO2014039119A1

    公开(公告)日：2014-03-13

    申请号：PCT/US2013/040424

    申请日：2013-05-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： GARGIR, Ari ,  DALLWIG, Jason ,  BEAUDET, Matthew

    IPC: G01N21/64

    CPC classification number: G01N21/6456 ,  G01J3/0208 ,  G01J3/0256 ,  G01J3/4406 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/062 ,  G01N2201/068

    Abstract: An apparatus for producing an image of blotting membranes includes an enclosure, light source, optical system, photodetector, and beamsplitter. The enclosure supports a blotting substrate comprising a first probe characterized by an excitation wavelength and an emission wavelength and a second probe characterized by an excitation wavelength and an emission wavelength. The light source directs diverging light to illuminate an entirety of the active area. The optical system forms an image of the entire active area and comprises an optical filter, the optical filter having an optical characteristic highly transmissive of light at the emission wavelengths and highly reflective of light at the excitation wavelengths. The beamsplitter may comprise an optical characteristic that is highly transmissive of light at the first and second emission wavelengths and that is highly reflective of light at the first and second excitation wavelengths.

    Abstract translation: 用于产生印迹膜的图像的设备包括外壳，光源，光学系统，光电检测器和分束器。 外壳支持吸墨衬底，其包括以激发波长和发射波长为特征的第一探针，以及以激发波长和发射波长为特征的第二探针。 光源引导发散的光照亮整个活动区域。 光学系统形成整个有源区域的图像并且包括滤光器，该滤光器具有在发射波长处高度透射光的光学特性，并且在激发波长处对光进行高度反射。 分束器可以包括对第一和第二发射波长的光高度透射并且高度反射第一和第二激发波长的光的光学特性。

    DETECTION AND QUANTIFICATION OF NUCLEIC ACID MOLECULES ASSOCIATED WITH A SURFACE
    2.
    发明申请
    DETECTION AND QUANTIFICATION OF NUCLEIC ACID MOLECULES ASSOCIATED WITH A SURFACE 审中-公开
    与表面相关的核酸分子的检测和定量

    公开(公告)号：WO2017100283A1

    公开(公告)日：2017-06-15

    申请号：PCT/US2016/065325

    申请日：2016-12-07

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

    Inventor： HEIL, Korbinian ,  NOTKA, Frank ,  GEE, Kyle ,  DALLWIG, Jason ,  KUHN, Phillip ,  CORRY, Schuyler

    IPC: C12Q1/68

    CPC classification number: C12Q1/6804 ,  C12Q1/6834 ,  C12Q2563/107 ,  C12Q2563/149 ,  C12Q2565/537

    Abstract: This disclosure relates to methods, compositions, and related apparatuses for detection and quantification of nucleic acid molecules associated with a solid surface. Methods can include detecting or quantifying at least one nucleic acid molecule associated with a surface by contacting the at least one nucleic acid molecule with a fluorophore, wherein the fluorophore emits increased fluorescence at a wavelength when in contact with nucleic acid molecule, and detecting or measuring fluorescence from the fluorophore at the wavelength. Compositions can comprise a fluorophore and a nucleic acid molecule, wherein the nucleic acid molecule is associated with a surface, and the fluorophore has the property of emitting increased fluorescence at a wavelength when in contact with a nucleic acid molecule.

    Abstract translation: 本公开涉及用于检测和定量与固体表面相关的核酸分子的方法，组合物和相关装置。 方法可以包括通过使至少一种核酸分子与荧光团接触来检测或定量至少一种与表面相关的核酸分子，其中荧光团在与核酸分子接触时在波长处发射增加的荧光，并且检测或测量 荧光团在波长处的荧光。 组合物可以包含荧光团和核酸分子，其中核酸分子与表面结合，并且荧光团在与核酸分子接触时具有在波长处发射增加的荧光的性质。

    SYSTEMS AND METHOD FOR FLUORESCENCE IMAGING
    7.
    发明公开
    SYSTEMS AND METHOD FOR FLUORESCENCE IMAGING 审中-公开
    系统和方法的荧光图像

    公开(公告)号：EP2893323A1

    公开(公告)日：2015-07-15

    申请号：EP13725007.2

    申请日：2013-05-09

    Applicant: Life Technologies Corporation

    Inventor： GARGIR, Ari ,  DALLWIG, Jason ,  BEAUDET, Matthew

    IPC: G01N21/64

    CPC classification number: G01N21/6456 ,  G01J3/0208 ,  G01J3/0256 ,  G01J3/4406 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/062 ,  G01N2201/068

    Abstract: An apparatus for producing an image of blotting membranes includes an enclosure, light source, optical system, photodetector, and beamsplitter. The enclosure supports a blotting substrate comprising a first probe characterized by an excitation wavelength and an emission wavelength and a second probe characterized by an excitation wavelength and an emission wavelength. The light source directs diverging light to illuminate an entirety of the active area. The optical system forms an image of the entire active area and comprises an optical filter, the optical filter having an optical characteristic highly transmissive of light at the emission wavelengths and highly reflective of light at the excitation wavelengths. The beamsplitter may comprise an optical characteristic that is highly transmissive of light at the first and second emission wavelengths and that is highly reflective of light at the first and second excitation wavelengths.

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