公开(公告)号：WO2016209753A1

公开(公告)日：2016-12-29

申请号：PCT/US2016/038305

申请日：2016-06-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MELQUIST, Penny ,  GEE, Kyle

IPC: G01N33/50 ,  G01N33/58 ,  C07D311/16 ,  C07D311/54 ,  C07F9/655 ,  C09B57/02

CPC classification number: G01N33/5005 ,  C09B57/02 ,  G01N33/582

Abstract: Described herein are compounds, methods, and kits for short- and long-term tracking of cell proliferation, differentiation, and/or function. The compounds disclosed herein are coumarin- based cell-tracking reagents that fluoresce in the blue portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, good retention within cells and low toxicity toward cells.

Abstract translation: 本文描述了用于短期和长期跟踪细胞增殖，分化和/或功能的化合物，方法和试剂盒。 本文公开的化合物是基于香豆素的细胞跟踪试剂，其在UV / VIS光谱的蓝色部分中发荧光，并提供明亮的荧光强度，均匀的细胞染色，细胞内的良好保留和对细胞的低毒性。

公开(公告)号：WO2013059475A1

公开(公告)日：2013-04-25

申请号：PCT/US2012/060832

申请日：2012-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KORE, Anilkumar ,  MUTHIAN, Shanmugasundaram ,  GEE, Kyle

IPC: C07H21/02 ,  C12N15/67

CPC classification number: C12N15/67 ,  A61K31/7084 ,  A61K31/7088 ,  A61K31/7115 ,  A61K31/712 ,  A61K39/12 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55511 ,  C07C247/04 ,  C07C323/60 ,  C07D213/82 ,  C07H1/00 ,  C07H1/02 ,  C07H1/04 ,  C07H19/207 ,  C07H21/02 ,  C12N7/00 ,  C12N15/11 ,  C12N2770/36134 ,  C12N2770/36171 ,  C12P19/34 ,  C12Q1/68

Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The "click" modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the "click" reaction are useful for producing RNA transcripts and encoded protein.

Abstract translation: 提供炔基衍生的帽类似物，炔基修饰的封端RNA，1,4-二取代的三唑衍生的封端RNA，制备方法，分离方法及其用途。 “点击”修改有助于检测和分离封闭的RNA，通过“点击”反应形成的1,4-二取代的三唑衍生物可用于产生RNA转录物和编码的蛋白质。

公开(公告)号：WO2012121973A1

公开(公告)日：2012-09-13

申请号：PCT/US2012/027285

申请日：2012-03-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  GEE, Kyle ,  SINGH, Upinder ,  GRECIAN, Scott

Inventor： GEE, Kyle ,  SINGH, Upinder ,  GRECIAN, Scott

IPC: C07D213/78

CPC classification number: G01N33/582 ,  C07D213/53 ,  C07D213/68 ,  C07D213/74 ,  C07D213/78 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D235/14 ,  C07D401/04 ,  C07D401/14 ,  C07K14/575 ,  C09B11/24 ,  C09B23/06 ,  C09B23/083 ,  G01N33/533

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

Abstract translation: 提供了低铜点击化学，1.3偶极环加成和用于修饰生物分子的Staudinger连接。 还提供了与低铜点击化学，1.3偶极环加成和Staudinger连接有关的组合物，方法和试剂盒。

公开(公告)号：WO2017040510A1

公开(公告)日：2017-03-09

申请号：PCT/US2016/049467

申请日：2016-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CLARKE, Scott ,  GEE, Kyle ,  FINNESSY, Carmen

IPC: C12Q1/68 ,  A61K47/48

CPC classification number: A61K49/0043 ,  A61K49/0054 ,  C12Q1/6806 ,  C12Q2525/101 ,  C12Q2525/117 ,  C12Q2525/197 ,  C12Q2565/1025

Abstract: The present disclosure provides methods, compositions and kits for measuring cellular nascent nucleic acid synthesis by colorimetric labeling of nucleic acid. The nucleic acid synthesis can be measured as cell proliferation, DNA or RNA synthesis, gene expression or apoptosis. Additionally, these methods may be used to screen compounds for their effect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.

Abstract translation: 本公开提供了通过核酸的比色标记测量细胞新生核酸合成的方法，组合物和试剂盒。 核酸合成可以作为细胞增殖，DNA或RNA合成，基因表达或凋亡来测定。 此外，这些方法可用于通过用竞争性核苷类似物在治疗前或之前用受试化合物处理细胞或生物体来筛选化合物对细胞增殖的影响。

公开(公告)号：WO2015175873A1

公开(公告)日：2015-11-19

申请号：PCT/US2015/030960

申请日：2015-05-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： GEE, Kyle ,  KANG, Hee Chol ,  ZHOU, Wenjun ,  DUBBELS, Bradley ,  OLSZOWY, Michael ,  O'GRADY, Michael ,  HUANG, Shih-jung ,  MELQUIST, Penny

IPC: G01N33/50 ,  G01N33/58 ,  C09B44/04 ,  C09B11/22 ,  C09B11/28

CPC classification number: C09B11/24 ,  A61K49/0041 ,  G01N33/5005 ,  G01N33/582

Abstract: Described herein are compounds, methods, and kits for short- and long-term tracking of cell proliferation, differentiation, and/or function. The compounds disclosed herein are rhodamine-based cell-tracking reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.

Abstract translation: 本文描述了用于短期和长期跟踪细胞增殖，分化和/或功能的化合物，方法和试剂盒。 本文公开的化合物是基于罗丹明的细胞跟踪试剂，其在UV / VIS光谱的红色部分发荧光，并提供明亮的荧光强度，均匀的细胞染色和在活细胞内的良好保留以及对细胞的低毒性。

公开(公告)号：WO2015175870A1

公开(公告)日：2015-11-19

申请号：PCT/US2015/030956

申请日：2015-05-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： GEE, Kyle ,  KANG, Hee Chol ,  ZHOU, Wenjun ,  DUBBELS, Bradley ,  OLSZOWY, Michael ,  O'GRADY, Michael

IPC: C07D471/04 ,  C07C251/24

CPC classification number: G01N33/5005 ,  C07C309/61 ,  C07C2603/24 ,  C07D471/04 ,  C07K16/2812 ,  C09B11/28 ,  G01N33/5014 ,  G01N33/5044

Abstract: Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.

Abstract translation: 本文描述的是荧光化合物和方法并且包含这些化合物。 本文公开的化合物是在紫外/可见光谱的红色部分中发荧光的卡吡泼因试剂，并提供明亮的荧光强度，均匀的细胞染色和活细胞内的良好保留以及对细胞的低毒性。

公开(公告)号：WO2014066733A2

公开(公告)日：2014-05-01

申请号：PCT/US2013/066765

申请日：2013-10-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： AGNEW, Brian ,  AGGELER, Robert ,  KANG, Hee Chol ,  CHEN, Aimei ,  GEE, Kyle

IPC: G01N33/50

CPC classification number: A61K51/10 ,  C07K14/435 ,  C07K16/18 ,  C12P21/005 ,  C12P21/06 ,  G01N33/534 ,  G01N33/582 ,  G01N33/6854

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

Abstract translation: 本文提供了用于糖蛋白的位点特异性标记的方法，组合物和试剂盒，其包含酶介导的包含化学处理和环加成化学的修饰的糖与标记分子的组合，所述标记分子包含 活性基团，金属离子螯合剂和/或荧光团。

公开(公告)号：WO2018085449A3

公开(公告)日：2018-05-11

申请号：PCT/US2017/059594

申请日：2017-11-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： GEE, Kyle ,  YANG, Jongtae

IPC: C07D417/12 ,  C09B23/04 ,  C12Q1/6818

Abstract: The present disclosure is directed to unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a quinolinium ring that contains a heteroatom. Compounds of formula (I) are provided herein. The compounds can be useful for fluorescent detection or quantification of nucleic acids. Related methods, uses, and kits are disclosed.

公开(公告)号：WO2018044688A1

公开(公告)日：2018-03-08

申请号：PCT/US2017/048467

申请日：2017-08-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： YORK, Adam ,  WELCH, Eric ,  GEE, Kyle ,  WANG, Xin ,  AGGELER, Robert ,  HU, Yi-Zhen

IPC: C09K11/02 ,  C09K11/06 ,  C08G61/10 ,  C08G61/12 ,  G01N33/533

Abstract: Water-soluble, fluorescent particles and compositions, kits, and methods of making and using such particles are disclosed. Processes for preparing fluorescent particles and for controlling the size, polydispersity and optical properties of such particles also are provided.

Abstract translation: 公开了水溶性荧光颗粒和组合物，试剂盒以及制造和使用这种颗粒的方法。 还提供了制备荧光颗粒和控制这些颗粒的尺寸，多分散性和光学性质的方法。

公开(公告)号：WO2018035230A1

公开(公告)日：2018-02-22

申请号：PCT/US2017/047152

申请日：2017-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BEACHAM, Daniel ,  GEE, Kyle ,  RUKAVISHNIKOV, Aleksey

IPC: G01N33/68 ,  C07D493/10

Abstract: Compositions and methods for detecting the activity of an ion channel in a cell are described. The methods include providing a loading buffer solution to the cell, where the loading buffer includes a thallium ion indicator and optionally chloride ions, and providing a stimulus buffer that includes thallium ions to the cell. Providing the stimulus buffer can cause thallium ion influx into or efflux out of the cell through the ion channel. After providing the stimulus buffer, a change in at least one optical property of the thallium ion indicator is detected in response to thallium influx or efflux, thereby detecting the activity of the ion channel.

Abstract translation: 描述了用于检测细胞中离子通道活性的组合物和方法。 该方法包括向细胞提供上样缓冲溶液，其中上样缓冲液包含铊离子指示剂和任选的氯离子，并向细胞提供包含铊离子的刺激缓冲液。 提供刺激缓冲液可引起铊离子通过离子通道流入或流出细胞。 在提供刺激缓冲液后，铊离子指示剂的至少一种光学性质的变化响应于铊流入或流出而被检测到，从而检测到离子通道的活性。