公开(公告)号：WO2018156880A1

公开(公告)日：2018-08-30

申请号：PCT/US2018/019399

申请日：2018-02-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LIM, Gary ,  LIU, David ,  LI, Peng ,  CAO, Bin ,  HIRANO, Kirk ,  DUKHOVNY, Alexander ,  DIXON, John

IPC: G01N27/447

Abstract: A biological analysis device (100) for performing capillary electrophoresis includes a voltage section (124) configured to generate a voltage differential across a cathode (106) connector and an anode (116) connector, an optical detector system (112) configured to detect light emission from a sample, a temperature regulation section (126), and a cartridge holding portion configured to receive at least a portion of a removable cartridge (102) comprising one or more capillaries (104) and a separation medium container (118). The biological analysis device may include one or more actuators (122) configured to actuate components of the removable cartridge when the removable cartridge is received in the cartridge holding portion. Devices and methods relate to biological analysis.

公开(公告)号：WO2015134945A1

公开(公告)日：2015-09-11

申请号：PCT/US2015/019310

申请日：2015-03-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WALTON, Ian ,  COBENE, Robert ,  DUKHOVNY, Alexander ,  SANNICANDRO, Adam ,  DIXON, John ,  KARGER, Achim ,  KLINE, Dan

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  G01N27/44721 ,  G01N27/44743 ,  G01N27/44756 ,  G01N35/10 ,  G01N35/1016

Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.

Abstract translation: 一方面，公开了一种生物测序装置，其包括构造成从仪器移除的盒。 在各种实施例中，筒可以包括适合于毛细管电泳的一个或多个毛细管，储存器和泵。 在各种实施方案中，储存器可以包含分离基质。 在各种实施例中，泵可以加载具有分离基质的毛细管。 在另一方面，生物测序装置可以包括一个或多个毛细管和一个集成的阀组件。 在各种实施例中，集成阀组件可以向一个或多个毛细管提供聚合物。

公开(公告)号：WO2015134943A1

公开(公告)日：2015-09-11

申请号：PCT/US2015/019307

申请日：2015-03-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WALTON, Ian ,  KARGER, Achim ,  KHORLIN, Alexander ,  SIMON, Michael ,  SANNICANDRO, Adam ,  DUKHOVNY, Alexander ,  COBENE, Robert ,  KLINE, Dan ,  DIXON, John

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44756

Abstract: The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. The system can also comprise an injection mechanism configured to deliver sample to the cathode end of the capillary array, and a temperature control zone, wherein the temperature control zone is configured to control the temperature of the interior of the housing.

Abstract translation: 在一些实施方案中，本公开涉及用于进行毛细管电泳测定的装置。 该装置可以包括包括阳极端和阴极端的毛细管阵列，毛细管阵列设置在壳体中，还包括构造成容纳分离介质和阳极缓冲器的储存器。 该系统还可以包括配置成将样品输送到毛细管阵列的阴极端的注射机构和温度控制区，其中温度控制区被配置为控制壳体内部的温度。

公开(公告)号：EP3114470A1

公开(公告)日：2017-01-11

申请号：EP15712219.3

申请日：2015-03-06

Applicant: Life Technologies Corporation

Inventor： WALTON, Ian ,  KARGER, Achim ,  KHORLIN, Alexander ,  SIMON, Michael ,  SANNICANDRO, Adam ,  DUKHOVNY, Alexander ,  COBENE, Robert ,  KLINE, Dan ,  DIXON, John

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44756

Abstract: The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. The system can also comprise an injection mechanism configured to deliver sample to the cathode end of the capillary array, and a temperature control zone, wherein the temperature control zone is configured to control the temperature of the interior of the housing.

Abstract translation: 在一些实施方案中，本公开涉及用于进行毛细管电泳测定的装置。 该装置可以包括包括阳极端和阴极端的毛细管阵列，毛细管阵列设置在壳体中，还包括构造成容纳分离介质和阳极缓冲器的储存器。 该系统还可以包括配置成将样品输送到毛细管阵列的阴极端的注射机构和温度控制区，其中温度控制区被配置为控制壳体内部的温度。

公开(公告)号：EP3586118A1

公开(公告)日：2020-01-01

申请号：EP18710219.9

申请日：2018-02-23

Applicant: Life Technologies Corporation

Inventor： LIM, Gary ,  LIU, David ,  LI, Peng ,  CAO, Bin ,  HIRANO, Kirk ,  DUKHOVNY, Alexander ,  DIXON, John

IPC: G01N27/447

公开(公告)号：EP3114471A1

公开(公告)日：2017-01-11

申请号：EP15712220.1

申请日：2015-03-06

Applicant: Life Technologies Corporation

Inventor： WALTON, Ian ,  COBENE, Robert ,  DUKHOVNY, Alexander ,  SANNICANDRO, Adam ,  DIXON, John ,  KARGER, Achim ,  KLINE, Dan

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  G01N27/44721 ,  G01N27/44743 ,  G01N27/44756 ,  G01N35/10 ,  G01N35/1016

Abstract: In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.

Abstract translation: 在一个方面，公开了一种生物测序装置，其包括被配置为从仪器移除的盒。 在各种实施例中，筒可以包括适合于毛细管电泳的一个或多个毛细管，储存器和泵。 在各种实施方案中，储存器可以包含分离基质。 在各种实施例中，泵可以加载具有分离基质的毛细管。 在另一方面，生物测序装置可以包括一个或多个毛细管和一个集成的阀组件。 在各种实施例中，集成阀组件可以向一个或多个毛细管提供聚合物。