公开(公告)号：WO2022155588A2

公开(公告)日：2022-07-21

申请号：PCT/US2022/012755

申请日：2022-01-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： SHANNON, Mark ,  ELDER, Noah ,  RAMAMOORTHI, Kalpith ,  LA, Jason ,  BLANCHARD, Daniel

IPC: C12Q1/6806

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the detection of target nucleic acids, including those from microbes and/or from infectious agents such as SARS-CoV-2 and other viruses. Embodiments described herein are designed to enable processing and analysis of the sample to detect target nucleic acids within the sample without requiring extraction and/or isolation of nucleic acid from the sample prior to subsequent processing steps. Samples analyzed can thus be "crude" biological samples that do not require pre-processing prior to placement in the workflow.

公开(公告)号：WO2023039525A1

公开(公告)日：2023-03-16

申请号：PCT/US2022/076204

申请日：2022-09-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： RAMAMOORTHI, Kalpith ,  ELDER, Noah

IPC: C12Q1/6806 ,  C12Q1/6832 ,  C12Q1/686

Abstract: Disclosed are master mix compositions, kits, and related methods for use in amplifying a target nucleic acid. A master mix composition may be formulated to enable effective reaction mixture loading onto a dPCR plate, provide high proportion of valid/readable partitions, enable identification and distinguishing between amplified and unamplified partitions, enable detection of low abundance targets amidst high background levels of non-target nucleic acids and/or amidst inhibiting agents, and/or enable effective detection of low frequency mutant alleles.

公开(公告)号：WO2022203748A1

公开(公告)日：2022-09-29

申请号：PCT/US2022/012805

申请日：2022-01-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WANG, Chunling ,  SHANG, Yulei ,  PABBATI, Namrata ,  LI, Kelly ,  PAGANI, Ioanna ,  BRZOSKA, Pius ,  TANNER, Michael ,  SHANNON, Mark ,  ELDER, Noah ,  RAMAMOORTHI, Kalpith ,  LA, Jason ,  BLANCHARD, Daniel ,  LI, Jisheng

IPC: C12Q1/6888 ,  C12Q2537/143

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：EP4314350A1

公开(公告)日：2024-02-07

申请号：EP22703496.4

申请日：2022-01-18

Applicant: Life Technologies Corporation

Inventor： WANG, Chunling ,  SHANG, Yulei ,  PABBATI, Namrata ,  LI, Kelly ,  PAGANI, Ioanna ,  BRZOSKA, Pius ,  TANNER, Michael ,  SHANNON, Mark ,  ELDER, Noah ,  RAMAMOORTHI, Kalpith ,  LA, Jason ,  BLANCHARD, Daniel ,  LI, Jisheng

IPC: C12Q1/6888

公开(公告)号：EP4278002A2

公开(公告)日：2023-11-22

申请号：EP22703196.0

申请日：2022-01-18

Applicant: Life Technologies Corporation

Inventor： SHANNON, Mark ,  ELDER, Noah ,  RAMAMOORTHI, Kalpith ,  LA, Jason ,  BLANCHARD, Daniel

IPC: C12Q1/6806