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公开(公告)号：WO2020056039A1
公开(公告)日：2020-03-19
申请号：PCT/US2019/050673
申请日：2019-09-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： HINZ, Wolfgang , DONOHUE, John , BEACHAM, Daniel
IPC: G01N27/414 , G01N33/487
Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells. The frame rate can be increased by selecting a smaller subset of pixels to monitor, i.e. by windowing down the area of a sensor array device over which data is collected.
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公开(公告)号：WO2018080925A1
公开(公告)日：2018-05-03
申请号：PCT/US2017/057641
申请日：2017-10-20
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： SHIN, Soojung , YAN, Yiping , BEACHAM, Daniel
IPC: C12N5/0793 , C12N5/00
CPC classification number: C12N5/0619 , C12N5/0031 , C12N5/0037 , C12N5/0606 , C12N5/0696 , C12N2500/99 , C12N2501/42 , C12N2506/08
Abstract: Embodiments herein provide methods of differentiating neural stem cells to neuronal cells while concomitantly retarding neural stem cell proliferation. Resultant cultures demonstrate reduced clumping of cells, increased purity of neuronal cells and accelerated electrophysiology as compared to control methods.
Abstract translation: 这里的实施方式提供了将神经干细胞分化成神经元细胞同时阻滞神经干细胞增殖的方法。 与对照方法相比，合成的培养物表现出细胞结块减少，神经元细胞纯度增加和电生理学加速。
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3.发明申请
公开(公告)号：WO2018035230A1
公开(公告)日：2018-02-22
申请号：PCT/US2017/047152
申请日：2017-08-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： BEACHAM, Daniel , GEE, Kyle , RUKAVISHNIKOV, Aleksey
IPC: G01N33/68 , C07D493/10
Abstract: Compositions and methods for detecting the activity of an ion channel in a cell are described. The methods include providing a loading buffer solution to the cell, where the loading buffer includes a thallium ion indicator and optionally chloride ions, and providing a stimulus buffer that includes thallium ions to the cell. Providing the stimulus buffer can cause thallium ion influx into or efflux out of the cell through the ion channel. After providing the stimulus buffer, a change in at least one optical property of the thallium ion indicator is detected in response to thallium influx or efflux, thereby detecting the activity of the ion channel.
Abstract translation: 描述了用于检测细胞中离子通道活性的组合物和方法。 该方法包括向细胞提供上样缓冲溶液，其中上样缓冲液包含铊离子指示剂和任选的氯离子，并向细胞提供包含铊离子的刺激缓冲液。 提供刺激缓冲液可引起铊离子通过离子通道流入或流出细胞。 在提供刺激缓冲液后，铊离子指示剂的至少一种光学性质的变化响应于铊流入或流出而被检测到，从而检测到离子通道的活性。
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公开(公告)号：WO2013180811A1
公开(公告)日：2013-12-05
申请号：PCT/US2013/031535
申请日：2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： GEE, Kyle , SINGH, Upinder , RUKAVISHNIKOV, Aleksey , BEACHAM, Daniel , HUANG, Shih-Jung , JANES, Michael , ZHOU, Wenjun , BERLIER, Judith
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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公开(公告)号：WO2013180813A1
公开(公告)日：2013-12-05
申请号：PCT/US2013/031637
申请日：2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： GEE, Kyle , SINGH, Upinder , RUKAVISHNIKOV, Aleksey , BEACHAM, Daniel , HUANG, Shih-Jung , JANES, Michael , ZHOU, Wenjun
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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Patent Agency Ranking
公开(公告)号：EP3500860A1
公开(公告)日：2019-06-26
申请号：EP17758368.9
申请日：2017-08-16
Applicant: Life Technologies Corporation
Inventor： BEACHAM, Daniel , GEE, Kyle , RUKAVISHNIKOV, Aleksey
IPC: G01N33/68 , C07D493/10
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7.发明公开FLUOROGENIC PH-SENSITIVE DYES AND THEIR METHODS OF USE (II) 审中-公开氟维森VERFAHREN ZUR VERWENDUNG（II）荧光感光纤维
公开(公告)号：EP2875075A1
公开(公告)日：2015-05-27
申请号：EP13713030.8
申请日：2013-03-14
Applicant: Life Technologies Corporation
Inventor： GEE, Kyle , SINGH, Upinder , RUKAVISHNIKOV, Aleksey , BEACHAM, Daniel , HUANG, Shih-Jung , JANES, Michael , ZHOU, Wenjun
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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公开(公告)号：EP3850350A1
公开(公告)日：2021-07-21
申请号：EP19773703.4
申请日：2019-09-11
Applicant: Life Technologies Corporation
Inventor： HINZ, Wolfgang , DONOHUE, John , BEACHAM, Daniel
IPC: G01N27/414 , G01N33/487
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公开(公告)号：EP2855596B1
公开(公告)日：2019-02-27
申请号：EP13714770.8
申请日：2013-03-14
Applicant: Life Technologies Corporation
Inventor： GEE, Kyle , SINGH, Upinder , RUKAVISHNIKOV, Aleksey , BEACHAM, Daniel , HUANG, Shih-Jung , JANES, Michael , ZHOU, Wenjun , BERLIER, Judith
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10.发明公开FLUOROGENIC pH-SENSITIVE DYES AND THEIR METHODS OF USE 审中-公开维生素敏感性芳香族维生素VERFAHREN ZU DEREN VERWENDUNG
公开(公告)号：EP2855596A1
公开(公告)日：2015-04-08
申请号：EP13714770.8
申请日：2013-03-14
Applicant: Life Technologies Corporation
Inventor： GEE, Kyle , SINGH, Upinder , RUKAVISHNIKOV, Aleksey , BEACHAM, Daniel , HUANG, Shih-Jung , JANES, Michael , ZHOU, Wenjun , BERLIER, Judith
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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