公开(公告)号：WO2015153973A1

公开(公告)日：2015-10-08

申请号：PCT/US2015/024249

申请日：2015-04-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WU, Yalei ,  KEYS, David

IPC: C12Q1/68

CPC classification number: G06F19/24 ,  C12Q1/6851 ,  G06F19/12 ,  G06F19/20 ,  G06F19/22 ,  G06F19/26 ,  C12Q2537/16 ,  C12Q2545/114

Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

Abstract translation: 提供了定量核酸的方法。 该方法包括确定用于参考核酸的第一预定输入量的第一参考阈值循环，确定靶核酸的第一预定输入量的第一目标阈值循环，为第二预定输入确定第二参考阈值循环 参考核酸的量，以及由处理器确定靶核酸的第二预定输入量的第二目标阈值循环。 该方法还包括接收采样阈值周期，基于第一和第二参考阈值周期以及第一和第二目标阈值周期来确定采样输入量，以及将样本输入量显示给用户。

公开(公告)号：WO2017053570A1

公开(公告)日：2017-03-30

申请号：PCT/US2016/053114

申请日：2016-09-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LI, Shifeng ,  DONG, Shoulian ,  STRAUB, Theodore ,  KEYS, David

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  C12Q1/6837 ,  C12Q1/6851 ,  G01N27/301 ,  G01N27/4145 ,  C12Q2531/113 ,  C12Q2563/116 ,  C12Q2563/159 ,  C12Q2565/607

Abstract: A system for detecting one or more target molecules includes a reaction device and a temperature controller. The reaction device comprises a plurality of reaction sites and plurality of field effect transistors corresponding to the plurality of reaction sites. At least two of the reaction sites may receive a reaction solution including two target molecules. The temperature controller is configured to control a temperature of at least one of the reaction sites or the reaction solution received by the at least one reaction site. At least some of the reaction sites may receive a reagent supporting an amplification reaction. When the reaction is subjected to an amplification assay, an amplified product is produced in the at least some of the reaction sites. The plurality of field effect transistors are configured to detect at least one of the amplified products.

Abstract translation: 用于检测一个或多个靶分子的系统包括反应装置和温度控制器。 反应装置包括多个反应部位和与多个反应部位对应的多个场效应晶体管。 至少两个反应位点可以接收包含两个靶分子的反应溶液。 温度控制器被配置为控制由至少一个反应位点接收的至少一个反应位点或反应溶液的温度。 至少一些反应位点可以接受支持扩增反应的试剂。 当反应进行扩增测定时，在至少一些反应位点产生扩增产物。 多个场效应晶体管被配置为检测至少一个放大产物。

公开(公告)号：WO2017059049A1

公开(公告)日：2017-04-06

申请号：PCT/US2016/054406

申请日：2016-09-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KEYS, David ,  MAJUMDAR, Nivedita Sumi ,  STRAUB, Theodore

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/024 ,  B01L2300/0829 ,  C12Q1/6851 ,  C12Q2527/107 ,  C12Q2545/114 ,  C12Q2561/113 ,  C12Q2563/159

Abstract: Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.

Abstract translation: 描述了用于定量靶核酸的系统和方法。 将包含靶核酸的样品分离成含有至少一个靶核酸分子的第一多个反应体积和不含靶核酸分子的第二多个反应体积。 对反应体积进行扩增测定，其中扩增测定被配置为扩增靶核酸。 在多个反应体积中的至少一些反应体积中检测或测量扩增指示物。 基于检测或测量量化靶核酸。 在停止扩增测定后，可以加热多个反应体积，并且可以检测或测量两种或更多种反应体积的扩增指标的变化。

公开(公告)号：EP3126518B1

公开(公告)日：2018-12-12

申请号：EP15716382.5

申请日：2015-04-03

Applicant: Life Technologies Corporation

Inventor： WU, Yalei ,  KEYS, David

IPC: C12Q1/6851 ,  G06F19/20

CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/16 ,  C12Q2545/114 ,  G06F19/12 ,  G06F19/20 ,  G06F19/22 ,  G06F19/24 ,  G06F19/26

Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

公开(公告)号：EP2689029A1

公开(公告)日：2014-01-29

申请号：EP12711104.5

申请日：2012-03-22

Applicant: Life Technologies Corporation

Inventor： KEYS, David

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q2527/146 ,  C12Q2537/143 ,  C12Q2549/119 ,  C12Q2565/102

Abstract: This specification generally relates to methods of detecting the linkage between two or more targets in a sample using digital multiplex PCR.

Abstract translation: 本说明书通常涉及使用数字多路复用PCR检测样品中两个或更多个目标之间的连接的方法。 提供了一种鉴定样品中两个或多个核酸靶物之间的物理连接的方法。 该方法包括通过有限稀释稀释样品，并将稀释的样品等分成孔。 该方法还包括在每个室中执行多重PCR，其中第一染料用于第一靶标，第二染料用于第二靶标。 该方法包括鉴定孔中第一和第二染料的存在，其中染料的非随机分布将目标标识为连接的。

公开(公告)号：EP3957398A1

公开(公告)日：2022-02-23

申请号：EP21183905.5

申请日：2016-09-29

Applicant: Life Technologies Corporation

Inventor： KEYS, David ,  MAJUMDAR, Nivedita Sumi ,  STRAUB, Theodore

IPC: B01L3/00 ,  C12Q1/686 ,  C12Q1/6851

Abstract: Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.

公开(公告)号：EP3356555A1

公开(公告)日：2018-08-08

申请号：EP16782341.8

申请日：2016-09-29

Applicant: Life Technologies Corporation

Inventor： KEYS, David ,  MAJUMDAR, Nivedita Sumi ,  STRAUB, Theodore

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/024 ,  B01L2300/0829 ,  C12Q1/6851 ,  C12Q2527/107 ,  C12Q2545/114 ,  C12Q2561/113 ,  C12Q2563/159

Abstract: Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.

公开(公告)号：EP3353323A1

公开(公告)日：2018-08-01

申请号：EP16777855.4

申请日：2016-09-22

Applicant: Life Technologies Corporation

Inventor： LI, Shifeng ,  DONG, Shoulian ,  STRAUB, Theodore ,  KEYS, David

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  C12Q1/6837 ,  C12Q1/6851 ,  G01N27/301 ,  G01N27/4145 ,  C12Q2531/113 ,  C12Q2563/116 ,  C12Q2563/159 ,  C12Q2565/607

Abstract: A system for detecting one or more target molecules includes a reaction device and a temperature controller. The reaction device comprises a plurality of reaction sites and plurality of field effect transistors corresponding to the plurality of reaction sites. At least two of the reaction sites may receive a reaction solution including two target molecules. The temperature controller is configured to control a temperature of at least one of the reaction sites or the reaction solution received by the at least one reaction site. At least some of the reaction sites may receive a reagent supporting an amplification reaction. When the reaction is subjected to an amplification assay, an amplified product is produced in the at least some of the reaction sites. The plurality of field effect transistors are configured to detect at least one of the amplified products.

公开(公告)号：EP3126518A1

公开(公告)日：2017-02-08

申请号：EP15716382.5

申请日：2015-04-03

Applicant: Life Technologies Corporation

Inventor： WU, Yalei ,  KEYS, David

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/16 ,  C12Q2545/114 ,  G06F19/12 ,  G06F19/20 ,  G06F19/22 ,  G06F19/24 ,  G06F19/26

Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

Abstract translation: 提供了定量核酸的方法。 该方法包括确定用于参考核酸的第一预定输入量的第一参考阈值循环，确定靶核酸的第一预定输入量的第一目标阈值循环，确定第二预定输入的第二参考阈值循环 参考核酸的量，以及由处理器确定靶核酸的第二预定输入量的第二目标阈值循环。 该方法还包括接收采样阈值周期，基于第一和第二参考阈值周期以及第一和第二目标阈值周期来确定采样输入量，以及将样本输入量显示给用户。