中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

20 records (took 0.044 seconds)

    SYSTEMS AND METHODS FOR PERFORMING DIGITAL PCR
    2.
    发明申请
    SYSTEMS AND METHODS FOR PERFORMING DIGITAL PCR 审中-公开
    用于执行数字PCR的系统和方法

    公开(公告)号：WO2017059049A1

    公开(公告)日：2017-04-06

    申请号：PCT/US2016/054406

    申请日：2016-09-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： KEYS, David ,  MAJUMDAR, Nivedita Sumi ,  STRAUB, Theodore

    IPC: C12Q1/68 ,  B01L3/00

    CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/024 ,  B01L2300/0829 ,  C12Q1/6851 ,  C12Q2527/107 ,  C12Q2545/114 ,  C12Q2561/113 ,  C12Q2563/159

    Abstract: Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.

    Abstract translation: 描述了用于定量靶核酸的系统和方法。 将包含靶核酸的样品分离成含有至少一个靶核酸分子的第一多个反应体积和不含靶核酸分子的第二多个反应体积。 对反应体积进行扩增测定，其中扩增测定被配置为扩增靶核酸。 在多个反应体积中的至少一些反应体积中检测或测量扩增指示物。 基于检测或测量量化靶核酸。 在停止扩增测定后，可以加热多个反应体积，并且可以检测或测量两种或更多种反应体积的扩增指标的变化。

    VISUALIZATION TOOLS FOR DIGITAL PCR DATA
    3.
    发明申请
    VISUALIZATION TOOLS FOR DIGITAL PCR DATA 审中-公开
    数字PCR数据可视化工具

    公开(公告)号：WO2014074735A2

    公开(公告)日：2014-05-15

    申请号：PCT/US2013/068984

    申请日：2013-11-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LEONG, Harrison ,  MAJUMDAR, Nivedita Sumi ,  MARKS, Jeffrey ,  STRAUB, Theodore ,  TALBOT, Ryan ,  BODNER, Kevin ,  HOARD, David

    IPC: G06F19/26

    CPC classification number: G06T11/206 ,  G06F19/26 ,  G06T11/203

    Abstract: A method for generating a data visualization is provided. The method includes displaying a representation of a portion of detected data from a substrate to a user. The method further includes generating a data quality value for the portion of detected data and displaying, along with the representation of the portion of detected data, an indication of data quality value for the portion of detected data. The method further includes selecting, by the user, a quality value threshold, and displaying an adjusted indication of data quality value for the portion of detected data meeting the quality value threshold.

    Abstract translation: 提供了一种用于生成数据可视化的方法。 该方法包括将检测到的数据的一部分从衬底显示给用户。 该方法还包括为检测到的数据的部分生成数据质量值，以及与检测到的数据的部分的表示一起显示检测到的数据的部分的数据质量值的指示。 该方法还包括由用户选择质量值阈值，并且显示对于满足质量值阈值的检测数据的部分的数据质量值的调整指示。

    SYSTEMS AND METHODS FOR ANALYSIS OF NUCLEIC ACIDS
    4.
    发明申请
    SYSTEMS AND METHODS FOR ANALYSIS OF NUCLEIC ACIDS 审中-公开
    核酸分析系统与方法

    公开(公告)号：WO2017053570A1

    公开(公告)日：2017-03-30

    申请号：PCT/US2016/053114

    申请日：2016-09-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LI, Shifeng ,  DONG, Shoulian ,  STRAUB, Theodore ,  KEYS, David

    IPC: C12Q1/68

    CPC classification number: C12Q1/6825 ,  C12Q1/6837 ,  C12Q1/6851 ,  G01N27/301 ,  G01N27/4145 ,  C12Q2531/113 ,  C12Q2563/116 ,  C12Q2563/159 ,  C12Q2565/607

    Abstract: A system for detecting one or more target molecules includes a reaction device and a temperature controller. The reaction device comprises a plurality of reaction sites and plurality of field effect transistors corresponding to the plurality of reaction sites. At least two of the reaction sites may receive a reaction solution including two target molecules. The temperature controller is configured to control a temperature of at least one of the reaction sites or the reaction solution received by the at least one reaction site. At least some of the reaction sites may receive a reagent supporting an amplification reaction. When the reaction is subjected to an amplification assay, an amplified product is produced in the at least some of the reaction sites. The plurality of field effect transistors are configured to detect at least one of the amplified products.

    Abstract translation: 用于检测一个或多个靶分子的系统包括反应装置和温度控制器。 反应装置包括多个反应部位和与多个反应部位对应的多个场效应晶体管。 至少两个反应位点可以接收包含两个靶分子的反应溶液。 温度控制器被配置为控制由至少一个反应位点接收的至少一个反应位点或反应溶液的温度。 至少一些反应位点可以接受支持扩增反应的试剂。 当反应进行扩增测定时，在至少一些反应位点产生扩增产物。 多个场效应晶体管被配置为检测至少一个放大产物。

    SYSTEMS AND METHODS FOR LOADING LIQUID SAMPLES
    5.
    发明申请
    SYSTEMS AND METHODS FOR LOADING LIQUID SAMPLES 审中-公开
    用于装载液体样品的系统和方法

    公开(公告)号：WO2015074076A1

    公开(公告)日：2015-05-21

    申请号：PCT/US2014/066225

    申请日：2014-11-18

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LIM, Gary ,  STRAUB, Theodore

    IPC: B01L3/02

    CPC classification number: B01L3/0293 ,  B01L3/50857 ,  B01L2200/026 ,  B01L2200/0642 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/12 ,  B01L2400/0406 ,  C12Q1/686

    Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85 +/- 15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action. The first and second blade may dispense the liquid by laterally moving over the plurality of reaction sites, where a motor laterally moves the first and second blade.

    Abstract translation: 提供了一种用于将液体样品加载到基底内的多个反应位点的样品装载器。 样品装载器包括第一叶片和联接到第一叶片的第二叶片。 样品装载器还包括在第一叶片和第二叶片之间的流动路径，其构造成将液体样品分配到包括多个反应位点的基底。 此外，在各种实施例中，液体样品与第一和第二叶片具有85 +/- 15度的前进接触角。 此外，从流动路径分配到多个反应位点的液体样品的装载可以基于毛细作用。 第一和第二叶片可以通过横向移动多个反应部位来分配液体，其中马达横向移动第一和第二叶片。

    CASE AND CASE HOLDER FOR BIOLOGICAL SAMPLES AND CORRESPONDING METHOD OF USE
    6.
    发明申请
    CASE AND CASE HOLDER FOR BIOLOGICAL SAMPLES AND CORRESPONDING METHOD OF USE 审中-公开
    用于生物样本的案例和案例持有人和相应的使用方法

    公开(公告)号：WO2014190357A1

    公开(公告)日：2014-11-27

    申请号：PCT/US2014/039623

    申请日：2014-05-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： PALLAS, Michael ,  FOSTER, Evan ,  FONSECA, Jorge ,  NURSE, James ,  STRAUB, Theodore

    IPC: B01L3/00

    CPC classification number: B01L3/50851 ,  B01L3/50857 ,  B01L7/52 ,  B01L9/523 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/141 ,  B01L2300/0609 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/18 ,  C12Q1/686 ,  G01N21/6452

    Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The support is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.

    Abstract translation: 用于处理多个生物样品的系统包含支架和温度控制器。 支撑件被构造成保持壳体，该壳体包括位于内室内的内室和基板，所述基板包含多个包含一个或多个生物样品的分离的反应位点。 温度控制器被配置为在一个或多个生物样品的测定或反应过程中维持或控制至少一种载体，壳体或一种或多种生物样品的温度。 支撑体还被配置为在测定或反应期间相对于水平面保持基板的至少一个表面为正角度。

    SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    7.
    发明申请
    SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS 审中-公开
    生物分析系统与方法

    公开(公告)号：WO2013138767A1

    公开(公告)日：2013-09-19

    申请号：PCT/US2013/032420

    申请日：2013-03-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： PALLAS, Michael, C. ,  NURSE, James ,  LIM, Gary ,  STRAUB, Theodore ,  GHENCIU, Eliodor ,  FOSTER, Evan ,  FONSECA, Jorge ,  MAHER, Kevin

    IPC: B01L7/00 ,  C12Q1/68 ,  B01L3/00

    CPC classification number: B01L3/50857 ,  B01L3/50 ,  B01L3/50851 ,  B01L2200/141 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  C12Q1/6806 ,  C12Q1/686 ,  G03F7/2002

    Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

    Abstract translation: 提供了用于进行生物反应的系统。 该系统包括包括基底和多个反应位点的芯片。 多个反应位点各自被配置为包括至多一个纳升级的液体样品。 此外，该系统包括被配置为启动液体样品内的生物反应的控制系统。 该系统还包括被配置为检测芯片上的生物反应的检测系统。 根据各种实施方案，该芯片包括至少20000个反应位点。 在其它实施方案中，芯片包括至少30000个反应位点。

    SYSTEMS AND METHODS FOR PERFORMING DIGITAL PCR
    9.
    发明公开
    SYSTEMS AND METHODS FOR PERFORMING DIGITAL PCR 审中-公开

    公开(公告)号：EP3957398A1

    公开(公告)日：2022-02-23

    申请号：EP21183905.5

    申请日：2016-09-29

    Applicant: Life Technologies Corporation

    Inventor： KEYS, David ,  MAJUMDAR, Nivedita Sumi ,  STRAUB, Theodore

    IPC: B01L3/00 ,  C12Q1/686 ,  C12Q1/6851

    Abstract: Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.

Patent Agency Ranking