公开(公告)号：US20130331276A1

公开(公告)日：2013-12-12

申请号：US13969195

申请日：2013-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. OLDHAM ,  George A. FRY ,  Christina E. INMAN ,  John BRIDGHAM ,  Timothy HUNKAPILLER ,  Charles S. VANN

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.

公开(公告)号：US20160305881A1

公开(公告)日：2016-10-20

申请号：US15065834

申请日：2016-03-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven J. BOEGE ,  Mark F. OLDHAM ,  Eugene F. YOUNG

IPC: G01N21/64

CPC classification number: G01N21/64 ,  F21K9/00 ,  F21K9/64 ,  F21V29/54 ,  F21V29/677 ,  F21V29/74 ,  F21Y2115/10 ,  G01J3/0286 ,  G01J3/10 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2201/0638 ,  G01N2201/1211 ,  G05D23/1919 ,  G05D23/20

Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with an excitation beam generated by a light source. A collimating lens can be disposed along a beam path between the light source and the reaction regions to form bundles of collimated excitation beams, wherein each bundle corresponds to a respective reaction region. Methods of analysis using the optical instrument are also provided.

公开(公告)号：US20140315206A1

公开(公告)日：2014-10-23

申请号：US14323203

申请日：2014-07-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. OLDHAM ,  Eric S. NORDMAN ,  Hongye SUN ,  Steven J. BOEGE

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

Abstract translation: 提供了一种扫描检测系统，其中检测到来自流动池中的位置的发射。 在一些实施例中，系统可以包括激发源，光切割源和调制光学器件，其被配置为使得由激发源产生的激发光束照射第一组固定位置并引起由光切割源产生的光致剪切光束 以照射与第一组固定位置分开并分开的第二组固定位置。 还提供了使用这种系统检测测序反应的方法。

公开(公告)号：US20190086334A1

公开(公告)日：2019-03-21

申请号：US16155831

申请日：2018-10-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. OLDHAM ,  Eric S. NORDMAN ,  Hongye SUN ,  Steven BOEGE

IPC: G01N21/64

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.