公开(公告)号：US20160130651A1

公开(公告)日：2016-05-12

申请号：US14997956

申请日：2016-01-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Dennis LEHTO ,  Steven J. BOEGE

IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/005 ,  B01J2219/00533 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01L3/502 ,  B01L3/50851 ,  B01L7/00 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/10 ,  B01L2300/043 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/1805 ,  B01L2300/1811 ,  B01L2300/1894 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N21/0332 ,  G01N21/05 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2201/02 ,  G03B27/42

Abstract: A device for performing biological sample reactions may include a plurality of flow cells configured to be mounted to a common microscope translation stage, wherein each flow cell is configured to receive at least one sample holder containing biological sample. Each flow cell also may be configured to be selectively placed in an open position for positioning the at least one sample holder into the flow cell and a closed position for reacting biological sample contained in the at least one sample holder. The plurality of flow cells may be configured to be selectively placed in the open position and the closed position independently of each other.

Abstract translation: 用于进行生物样品反应的装置可以包括多个流动池，其构造成安装到普通的显微镜平移台上，其中每个流动池被构造成容纳至少一个含有生物样品的样品架。 每个流动池还可以被配置为选择性地放置在打开位置，用于将至少一个样品保持器定位到流动室中，以及用于使包含在至少一个样品保持器中的生物样品反应的关闭位置。 多个流动池可以被配置为被选择性地彼此相对放置在打开位置和关闭位置。

公开(公告)号：US20140315206A1

公开(公告)日：2014-10-23

申请号：US14323203

申请日：2014-07-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. OLDHAM ,  Eric S. NORDMAN ,  Hongye SUN ,  Steven J. BOEGE

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

Abstract translation: 提供了一种扫描检测系统，其中检测到来自流动池中的位置的发射。 在一些实施例中，系统可以包括激发源，光切割源和调制光学器件，其被配置为使得由激发源产生的激发光束照射第一组固定位置并引起由光切割源产生的光致剪切光束 以照射与第一组固定位置分开并分开的第二组固定位置。 还提供了使用这种系统检测测序反应的方法。

公开(公告)号：US20160305881A1

公开(公告)日：2016-10-20

申请号：US15065834

申请日：2016-03-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven J. BOEGE ,  Mark F. OLDHAM ,  Eugene F. YOUNG

IPC: G01N21/64

CPC classification number: G01N21/64 ,  F21K9/00 ,  F21K9/64 ,  F21V29/54 ,  F21V29/677 ,  F21V29/74 ,  F21Y2115/10 ,  G01J3/0286 ,  G01J3/10 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2201/0638 ,  G01N2201/1211 ,  G05D23/1919 ,  G05D23/20

Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with an excitation beam generated by a light source. A collimating lens can be disposed along a beam path between the light source and the reaction regions to form bundles of collimated excitation beams, wherein each bundle corresponds to a respective reaction region. Methods of analysis using the optical instrument are also provided.