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    PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF
    1.
    发明申请
    PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF 审中-公开
    用于细胞培养的球体及其制备方法

    公开(公告)号：WO2017106783A1

    公开(公告)日：2017-06-22

    申请号：PCT/US2016/067374

    申请日：2016-12-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： PHELPS, Mwita ,  GULDE, Paul ,  FIKE, Richard ,  REYNOLDS, Mary ,  HASSETT, Richard ,  CAMPBELL, Andrew

    IPC: C12N5/00 ,  C12N1/22

    CPC classification number: C12N5/0018 ,  B01J8/1845 ,  C12N1/22 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/60

    Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.

    Abstract translation: 本发明总体上涉及干细胞培养基或颗粒形式的饲料，其可以重构成液体培养基用于体外培养细胞。 每种丸粒组合物可以包含相同或不同的组合物; 例如不同的维生素，氨基酸，缓冲剂，微量盐，pH，铁螯合剂等。本发明还涉及通过改变不同丸粒组合物的比例来制备干细胞培养基的方法，或制备模块化干细胞培养物 培养基或使用颗粒定制培养细胞类型的培养基配方。 根据本发明，介质丸粒在重构之前，运输和处理过程中可能更容易处理; 和/或在重建期间。 培养基颗粒可用于任何容器中，如袋子，包括用于制备培养基制剂的无菌一次性袋子。 本发明还涉及使用培养基颗粒的试剂盒和培养系统。

    LAYERED CELL CULTURE PARTICLES AND METHODS OF MAKING THEREOF
    3.
    发明申请
    LAYERED CELL CULTURE PARTICLES AND METHODS OF MAKING THEREOF 审中-公开
    层状细胞培养颗粒及其制备方法

    公开(公告)号：WO2017117559A1

    公开(公告)日：2017-07-06

    申请号：PCT/US2016/069564

    申请日：2016-12-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： PHELPS, Mwita ,  GULDE, Paul ,  FIKE, Richard ,  REYNOLDS, Mary ,  HASSETT, Richard

    IPC: C12N1/22 ,  C12N5/00

    CPC classification number: C12N5/0018 ,  B01J2/006 ,  B01J2/16

    Abstract: The present invention is directed to dry cell culture media or feeds comprising layered particles. The less stable or sensitive components are separated spatially from reactive components in media, feeds, supplements, or concentrates due to layering. The invention relates to processes for preparing such layered compositions and methods of making cell culture compositions that are stable, thermally, photo-chemically and/or to gamma irradiation. The invention also relates to kits and culture systems using media layered particles.

    Abstract translation: 本发明涉及包含分层颗粒的干细胞培养基或饲料。 由于分层，不太稳定或敏感的组分在空间上与介质，进料，补充剂或浓缩物中的反应组分分离。 本发明涉及制备这种分层组合物的方法以及制备稳定的，热的，光化学的和/或γ射线照射的细胞培养组合物的方法。 本发明还涉及使用介质分层颗粒的试剂盒和培养系统。

    PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF
    4.
    发明公开
    PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF 审中-公开

    公开(公告)号：EP4036220A1

    公开(公告)日：2022-08-03

    申请号：EP21212458.0

    申请日：2016-12-16

    Applicant: Life Technologies Corporation

    Inventor： PHELPS, Mwita ,  GULDE, Paul ,  FIKE, Richard ,  REYNOLDS, Mary ,  HASSETT, Richard ,  CAMPBELL, Andrew

    IPC: C12N5/00 ,  C12N1/22 ,  C12M1/12

    Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for ex-ample, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.

    PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF
    7.
    发明公开
    PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF 审中-公开
    用于细胞培养的球体及其制备方法

    公开(公告)号：EP3271452A1

    公开(公告)日：2018-01-24

    申请号：EP16822591.0

    申请日：2016-12-16

    Applicant: Life Technologies Corporation

    Inventor： PHELPS, Mwita ,  GULDE, Paul ,  FIKE, Richard ,  REYNOLDS, Mary ,  HASSETT, Richard ,  CAMPBELL, Andrew

    IPC: C12N5/00 ,  C12N1/22

    CPC classification number: C12N5/0018 ,  B01J8/1845 ,  C12N1/22 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/60

    Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.

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